Agriculture is still the biggest contributor of non-point source (NPS) pollution to water bodies andrunoff discharges of nutrients and other chemicals are one of the most important pathways. This studywas conducted du...Agriculture is still the biggest contributor of non-point source (NPS) pollution to water bodies andrunoff discharges of nutrients and other chemicals are one of the most important pathways. This studywas conducted during 1998~1999 in a typical watershed with complex agriculture and forestry systems aswell as dotted farmer villages, in a transitional region between the mid- and northern subtropical zones ofChina. Continuous sampling of stream water was performed regularly at a weekly frequency, with additionalsampling after all major rainfall events. The discharges of N and P nutrients and suspended materials weremeasured and the total and area-averaged annual discharges of all components were calculated. The resultsshowed an uneven seasonal distribution of nutrient discharges with summer storms contributing most tothe total fluxes. This study demonstrated a high dependence of runoff volume on rainfall but the overallrunoff coefficients were dependent on land use type and watershed size. The area-averaged annual dischargesvaried greatly among the sub-watersheds with different sizes and land use structures. This is the first studyestimating the area-averaged annual discharges of N and P in the hilly areas of subtropical China, which were1.5 g m-2 a-1 and 0.1 g m-2 a-1, respectively, providing important reference values for the assessment ofregional agricultural non-point source pollution.展开更多
To improve current understanding of the water cycle,energy partitioning and CO2 exchange over hilly zone vegetative land surfaces in the subtropical monsoon environment of southern China,a long-term field experiment o...To improve current understanding of the water cycle,energy partitioning and CO2 exchange over hilly zone vegetative land surfaces in the subtropical monsoon environment of southern China,a long-term field experiment observatory was set up at Ningxiang,eastern Hunan Province.This paper presents a preliminary analysis of the field observations at the observatory collected from August to November 2012.Results show that significant diurnal variations in soil temperature occur only in shallow soil layers(0.05,0.10,and 0.20 m),and that heavy rainfall affects soil moisture in the deep layers(≥ 0.40 m).During the experimental period,significant diurnal variations in albedo,radiation components,energy components,and CO2 flux were observed,but little seasonal variation.Strong photosynthesis in the vegetation canopy enhanced the CO2 absorption and the latent heat released in daylight hours;Latent heat of evaporation was the main consumer of available energy in late summer.Because the field experiment data are demonstrably reliable,the observatory will provide reliable long-term measurements for future investigations of the land-atmosphere interaction over hilly land surfaces in the subtropical monsoon region of southern China.展开更多
Collaboration of interannual variabilities and the climate mean state determines the type of E1 Nifio. Recent studies highlight the impact of a La Nifia-like mean state change, which acts to suppress the convection an...Collaboration of interannual variabilities and the climate mean state determines the type of E1 Nifio. Recent studies highlight the impact of a La Nifia-like mean state change, which acts to suppress the convection and low-level convergence over the central Pacific, on the predominance of central Pacific (CP) E1 Nifio in the most recent decade. However, how interannual variabilities affect the climate mean state has been less thoroughly investigated. Using a linear shallow-water model, the ef- fect of decadal changes of air-sea interaction on the two types of El Nifio and the climate mean state over the tropical Pacific is examined. It is demonstrated that the predominance of the eastem Pacific (EP) and CP E1 Nino is dominated mainly by relationships between anomalous wind stresses and sea surface temperature (SST). Furthermore, changes between air-sea interactions from 1980-98 to 1999-2011 prompted the generation of the La Ninalike pattern, which is similar to the background change in the most recent decade.展开更多
Guava leaf tea has been used as a folk medicine for treating hyperglycemic conditions in Asia and Africa. The hypoglycemic efficacy of guava leaf has been documented by many scientists in these regions, but the hypogl...Guava leaf tea has been used as a folk medicine for treating hyperglycemic conditions in Asia and Africa. The hypoglycemic efficacy of guava leaf has been documented by many scientists in these regions, but the hypoglycemic mechanism is poorly understood. Guava leaves were extracted with methanol and the crude extract was partitioned against hexane, ethyl acetate, and butanol in sequence. The leftover in water is defined as the aqueous partition. A second smaller batch was extracted with hot water directly. Oral glucose tolerance test was carried out on healthy mice instead of diabetic mice that lack endogenous insulin. Glucose uptake was examined with 3T3-L1 adipocytes. Oxidative effect on PTP1B (protein tyrosine phosphatase 1b) was carried out with real-time PTP1B enzymatic assay. The aqueous partition of guava leaf extract possesses a potent inhibitory effect on PTP1B enzymatic activity and this PTP1B inhibition is through a slow oxidative but reversible inactivation on the enzyme. The reversible inactivation would suggest guava leaf extract may augment PTP1B inhibition alongside the endogenous H2O2 which itself is induced by insulin. In addition, our study confirmed the hypoglycemic efficacy being associated with guava leaf and found the most effective molecules reside in the aqueous partition which is also less cytotoxic to Chinese hamster ovary cells when compared to other less polar partitions. The guava leaf extract can modulate insulin activity through a redox regulation on PP1B enzymatic activity. It is speculated that a compound similar to gallocatechin in the aqueous partition can reduce an oxygen molecule to hydrogen peroxide which in turn oxidizes the catalytic residue Cys in PTP1B. Therefore, the guava leaf tea can serve as a functional hypoglycemic drink that is suitable for either healthy or diabetic subjects.展开更多
The laminar heat transfer in the thermal entrance region in round tubes, which has a variable surface heat flux boundary condition, is analytically studied. The results show that the heat transfer coefficient is close...The laminar heat transfer in the thermal entrance region in round tubes, which has a variable surface heat flux boundary condition, is analytically studied. The results show that the heat transfer coefficient is closely related to the wall temperature gradient along the tube axis. The greater the gradient, the higher the heat transfer rate. Furthermore, the coordination of the velocity and the temperature gradient fields is also analysed under different surface heat fluxes. The validity of the field coordination principle is verified by checking the correlation of heat transfer coefficient and the coordination degree. The results also demonstrate that optimizing the thermal boundary condition is a way to enhance heat transfer.展开更多
Soil microbiological and biochemical properties under various field crop rotations such as grains, pastures and vegetables have been studied intensively under short-term period. However, there is limited information a...Soil microbiological and biochemical properties under various field crop rotations such as grains, pastures and vegetables have been studied intensively under short-term period. However, there is limited information about the influence of banana-based rotations on soil organic C, total N(TN), microbial biomasses and enzyme activities under long-term crop rotations. A field experiment arranged in a randomized complete block design with three replicates was carried out at the Wanzhong Farm in Ledong(18?37′–18?38′N, 108?46′–108?48′E), Hainan Province, China, to compare the responses of these soil parameters to long-term(10-year) banana(Musa paradisiaca)-pineapple(Ananas) rotation(AB), banana-papaya(Carica) rotation(BB) and banana monoculture(CK) in a conventional tillage system in the Hainan Island. Soil p H, total organic C(TOC), dissolved organic C(DOC), TN, total P(TP) and available P(AP) were found to be significantly higher(P < 0.01) in AB and BB than CK at 0–30 cm soil depth. Microbial biomass C(MBC) and N(MBN) were observed 18.0%–35.2% higher in AB and 8.6%–40.5% higher in BB than CK at 0–30 cm. The activities of urease(UA), invertase(IA), dehydrogenase(DA) and acid phosphatase(APA) showed a mean of 21.5%–59.6% increase in AB and 26.7%–66.1% increase in BB compared with CK at 0–30 cm. Higher p H, TOC and DOC at 0–10 and 10–20 cm than at 20–30 cm were obtained despite of the rotations. Soil MBC and MBN and activities of UA, IA and DA decreased markedly(P < 0.01) with increasing soil depth in the different rotation soils as well as the monoculture soil. In general, soil microbial biomass and enzymatic activities were more sensitive to changes in banana-based rotations than soil chemical properties, and consequently they were well-established as early indicators of changes due to crop rotations in the tropics.展开更多
A field experiment was carried out from 2003 to 2013 in the Wanzhong Farm of the Hainan Island, China, to determine the effects of two long-term banana rotations on the abundance and trophic groups of soil nematode co...A field experiment was carried out from 2003 to 2013 in the Wanzhong Farm of the Hainan Island, China, to determine the effects of two long-term banana rotations on the abundance and trophic groups of soil nematode communities in the island. The experiment was set out as a randomized complete block design with three replications of three treatments: banana-pineapple rotation (AB), banana-papaya rotation (BB) and banana monoculture (CK) in a conventional tillage system. Soil samples were taken at depths of 0-10, 10-20 and 20-30 cm, and nematodes were extracted by a modified cotton-wool filter method and identified to the genus level. Nematode ecological indices of Shannon-Weaver diversity (Ht), dominance index (A), maturity index (MI), plant parasite index (PPI), structure index (SI), enrichment index (EI), and channel index (CI) were calculated. A total of 28 nematode genera with relative abundance over 0.1% were identified, among which Tylenchus and Paratylenchus in the AB, Thonus in the BB, Tylenchus and Helicotylenchus in the CK were the dominant genera. The rotation soils favored bacterivores, fungivores and omnivores-predators with high colonizer-persister (c-p) values. Soil food web in the rotation systems was highly structured, mature and enriched as indicated by SI, MI and EI values, respectively. Higher abundance of bacterivores and lower values of CI suggested that the soil food web was dominated by a bacterial decomposition pathway in rotation soils. Nematode diversity was much higher after a decade of rotation. Soil depth had significant effects on the abundance of soil nematodes, but only on two nematode ecological indices (λ and MI).展开更多
Thiazolidinediones(TZDs),such as rosiglitazone(RSG),which activates peroxisome proliferator activated receptor-y(PPARy),are a potent class of oral antidiabetic agents with good durability.However,the clinical use of T...Thiazolidinediones(TZDs),such as rosiglitazone(RSG),which activates peroxisome proliferator activated receptor-y(PPARy),are a potent class of oral antidiabetic agents with good durability.However,the clinical use of TZDs is challenging because of their side effects,including weight gain and hepatotoxicity.Here,we found that bavachinin(BVC),a lead natural product,additively activates PPARγ with lowdose RSG to preserve the maximum antidiabetic effects while reducing weight gain and hepatotoxicity in db/db mice caused by RSG monotherapy.Structural and biochemical assays demonstrated that an unexplored hotspot around Met329 and Ser332 in helix 5 is triggered by BVC cobinding to RSG-bound PPARy,thereby allosterically stabilizing the active state of the activation-function 2 motif responsible for additive activation with RSG.Based on this hotspot,we discovered a series of new classes of allosteric agonists inducing the activity of TZDs in the same manner as BVC.Together,our data illustrate that the hotspot of PPARγ is druggable for the discovery of new allosteric synergists,and the combination thera py of allosteric synergists and TZD drugs may provide a potential alternative approach to the treatment of type 2 diabetes mellitus.展开更多
基金Project supported by the International Foundation of Science(No.C/2661-1)the National Key Basic Research Support Foundation of China(No.G1999011809).
文摘Agriculture is still the biggest contributor of non-point source (NPS) pollution to water bodies andrunoff discharges of nutrients and other chemicals are one of the most important pathways. This studywas conducted during 1998~1999 in a typical watershed with complex agriculture and forestry systems aswell as dotted farmer villages, in a transitional region between the mid- and northern subtropical zones ofChina. Continuous sampling of stream water was performed regularly at a weekly frequency, with additionalsampling after all major rainfall events. The discharges of N and P nutrients and suspended materials weremeasured and the total and area-averaged annual discharges of all components were calculated. The resultsshowed an uneven seasonal distribution of nutrient discharges with summer storms contributing most tothe total fluxes. This study demonstrated a high dependence of runoff volume on rainfall but the overallrunoff coefficients were dependent on land use type and watershed size. The area-averaged annual dischargesvaried greatly among the sub-watersheds with different sizes and land use structures. This is the first studyestimating the area-averaged annual discharges of N and P in the hilly areas of subtropical China, which were1.5 g m-2 a-1 and 0.1 g m-2 a-1, respectively, providing important reference values for the assessment ofregional agricultural non-point source pollution.
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDA05110102)the National Natural Science Foundation of China (Grant No.41075062)the National Basic Research Program of China (Grant No. 2010CB951001)
文摘To improve current understanding of the water cycle,energy partitioning and CO2 exchange over hilly zone vegetative land surfaces in the subtropical monsoon environment of southern China,a long-term field experiment observatory was set up at Ningxiang,eastern Hunan Province.This paper presents a preliminary analysis of the field observations at the observatory collected from August to November 2012.Results show that significant diurnal variations in soil temperature occur only in shallow soil layers(0.05,0.10,and 0.20 m),and that heavy rainfall affects soil moisture in the deep layers(≥ 0.40 m).During the experimental period,significant diurnal variations in albedo,radiation components,energy components,and CO2 flux were observed,but little seasonal variation.Strong photosynthesis in the vegetation canopy enhanced the CO2 absorption and the latent heat released in daylight hours;Latent heat of evaporation was the main consumer of available energy in late summer.Because the field experiment data are demonstrably reliable,the observatory will provide reliable long-term measurements for future investigations of the land-atmosphere interaction over hilly land surfaces in the subtropical monsoon region of southern China.
基金supported by the National Program for Support of Top-notch Young Professionals,the National Basic Research Program of China (Grant Nos. 2012CB955202 and 2012CB417404)"Western Pacific Ocean System: Structure, Dynamics, and Consequences" of the Chinese Academy Sciences (WPOS+1 种基金 Grant No. XDA10010405)the National Natural Science Foundation of China (Grant No. 41176014)
文摘Collaboration of interannual variabilities and the climate mean state determines the type of E1 Nifio. Recent studies highlight the impact of a La Nifia-like mean state change, which acts to suppress the convection and low-level convergence over the central Pacific, on the predominance of central Pacific (CP) E1 Nifio in the most recent decade. However, how interannual variabilities affect the climate mean state has been less thoroughly investigated. Using a linear shallow-water model, the ef- fect of decadal changes of air-sea interaction on the two types of El Nifio and the climate mean state over the tropical Pacific is examined. It is demonstrated that the predominance of the eastem Pacific (EP) and CP E1 Nino is dominated mainly by relationships between anomalous wind stresses and sea surface temperature (SST). Furthermore, changes between air-sea interactions from 1980-98 to 1999-2011 prompted the generation of the La Ninalike pattern, which is similar to the background change in the most recent decade.
文摘Guava leaf tea has been used as a folk medicine for treating hyperglycemic conditions in Asia and Africa. The hypoglycemic efficacy of guava leaf has been documented by many scientists in these regions, but the hypoglycemic mechanism is poorly understood. Guava leaves were extracted with methanol and the crude extract was partitioned against hexane, ethyl acetate, and butanol in sequence. The leftover in water is defined as the aqueous partition. A second smaller batch was extracted with hot water directly. Oral glucose tolerance test was carried out on healthy mice instead of diabetic mice that lack endogenous insulin. Glucose uptake was examined with 3T3-L1 adipocytes. Oxidative effect on PTP1B (protein tyrosine phosphatase 1b) was carried out with real-time PTP1B enzymatic assay. The aqueous partition of guava leaf extract possesses a potent inhibitory effect on PTP1B enzymatic activity and this PTP1B inhibition is through a slow oxidative but reversible inactivation on the enzyme. The reversible inactivation would suggest guava leaf extract may augment PTP1B inhibition alongside the endogenous H2O2 which itself is induced by insulin. In addition, our study confirmed the hypoglycemic efficacy being associated with guava leaf and found the most effective molecules reside in the aqueous partition which is also less cytotoxic to Chinese hamster ovary cells when compared to other less polar partitions. The guava leaf extract can modulate insulin activity through a redox regulation on PP1B enzymatic activity. It is speculated that a compound similar to gallocatechin in the aqueous partition can reduce an oxygen molecule to hydrogen peroxide which in turn oxidizes the catalytic residue Cys in PTP1B. Therefore, the guava leaf tea can serve as a functional hypoglycemic drink that is suitable for either healthy or diabetic subjects.
文摘The laminar heat transfer in the thermal entrance region in round tubes, which has a variable surface heat flux boundary condition, is analytically studied. The results show that the heat transfer coefficient is closely related to the wall temperature gradient along the tube axis. The greater the gradient, the higher the heat transfer rate. Furthermore, the coordination of the velocity and the temperature gradient fields is also analysed under different surface heat fluxes. The validity of the field coordination principle is verified by checking the correlation of heat transfer coefficient and the coordination degree. The results also demonstrate that optimizing the thermal boundary condition is a way to enhance heat transfer.
基金supported by the National Natural Science Foundation of China (No. 41301277)the Natural Science Foundation of Hainan Province, China (No. 310073)
文摘Soil microbiological and biochemical properties under various field crop rotations such as grains, pastures and vegetables have been studied intensively under short-term period. However, there is limited information about the influence of banana-based rotations on soil organic C, total N(TN), microbial biomasses and enzyme activities under long-term crop rotations. A field experiment arranged in a randomized complete block design with three replicates was carried out at the Wanzhong Farm in Ledong(18?37′–18?38′N, 108?46′–108?48′E), Hainan Province, China, to compare the responses of these soil parameters to long-term(10-year) banana(Musa paradisiaca)-pineapple(Ananas) rotation(AB), banana-papaya(Carica) rotation(BB) and banana monoculture(CK) in a conventional tillage system in the Hainan Island. Soil p H, total organic C(TOC), dissolved organic C(DOC), TN, total P(TP) and available P(AP) were found to be significantly higher(P < 0.01) in AB and BB than CK at 0–30 cm soil depth. Microbial biomass C(MBC) and N(MBN) were observed 18.0%–35.2% higher in AB and 8.6%–40.5% higher in BB than CK at 0–30 cm. The activities of urease(UA), invertase(IA), dehydrogenase(DA) and acid phosphatase(APA) showed a mean of 21.5%–59.6% increase in AB and 26.7%–66.1% increase in BB compared with CK at 0–30 cm. Higher p H, TOC and DOC at 0–10 and 10–20 cm than at 20–30 cm were obtained despite of the rotations. Soil MBC and MBN and activities of UA, IA and DA decreased markedly(P < 0.01) with increasing soil depth in the different rotation soils as well as the monoculture soil. In general, soil microbial biomass and enzymatic activities were more sensitive to changes in banana-based rotations than soil chemical properties, and consequently they were well-established as early indicators of changes due to crop rotations in the tropics.
基金supported by the National Natural Science Foundation of China (No. 41301277)the Natural Science Foundation of Hainan Province, China (No. 310073)
文摘A field experiment was carried out from 2003 to 2013 in the Wanzhong Farm of the Hainan Island, China, to determine the effects of two long-term banana rotations on the abundance and trophic groups of soil nematode communities in the island. The experiment was set out as a randomized complete block design with three replications of three treatments: banana-pineapple rotation (AB), banana-papaya rotation (BB) and banana monoculture (CK) in a conventional tillage system. Soil samples were taken at depths of 0-10, 10-20 and 20-30 cm, and nematodes were extracted by a modified cotton-wool filter method and identified to the genus level. Nematode ecological indices of Shannon-Weaver diversity (Ht), dominance index (A), maturity index (MI), plant parasite index (PPI), structure index (SI), enrichment index (EI), and channel index (CI) were calculated. A total of 28 nematode genera with relative abundance over 0.1% were identified, among which Tylenchus and Paratylenchus in the AB, Thonus in the BB, Tylenchus and Helicotylenchus in the CK were the dominant genera. The rotation soils favored bacterivores, fungivores and omnivores-predators with high colonizer-persister (c-p) values. Soil food web in the rotation systems was highly structured, mature and enriched as indicated by SI, MI and EI values, respectively. Higher abundance of bacterivores and lower values of CI suggested that the soil food web was dominated by a bacterial decomposition pathway in rotation soils. Nematode diversity was much higher after a decade of rotation. Soil depth had significant effects on the abundance of soil nematodes, but only on two nematode ecological indices (λ and MI).
基金the National Natural Science Foundation of China(21708025,81925034,91753117,and 81773793)the Open Fund of State Key Laboratory of Oncogenes and Related Genes,Shanghai Jiao Tong University School of Medicine+3 种基金the Innovation Program of Shanghai Municipal Education Commission(2019-01-07-00-01-E00036)the Shanghai Science and Technology Innovation Foundation(19431901600)the China Postdoctoral Science Foundation(2016M601618 and 2017T100303)the National Science and Technology Major Project of China(2018ZX09711001-005-022)。
文摘Thiazolidinediones(TZDs),such as rosiglitazone(RSG),which activates peroxisome proliferator activated receptor-y(PPARy),are a potent class of oral antidiabetic agents with good durability.However,the clinical use of TZDs is challenging because of their side effects,including weight gain and hepatotoxicity.Here,we found that bavachinin(BVC),a lead natural product,additively activates PPARγ with lowdose RSG to preserve the maximum antidiabetic effects while reducing weight gain and hepatotoxicity in db/db mice caused by RSG monotherapy.Structural and biochemical assays demonstrated that an unexplored hotspot around Met329 and Ser332 in helix 5 is triggered by BVC cobinding to RSG-bound PPARy,thereby allosterically stabilizing the active state of the activation-function 2 motif responsible for additive activation with RSG.Based on this hotspot,we discovered a series of new classes of allosteric agonists inducing the activity of TZDs in the same manner as BVC.Together,our data illustrate that the hotspot of PPARγ is druggable for the discovery of new allosteric synergists,and the combination thera py of allosteric synergists and TZD drugs may provide a potential alternative approach to the treatment of type 2 diabetes mellitus.