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尖峰岭抗逆性根瘤菌的筛选及其16S rDNA序列的测定 被引量:3
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作者 彭玉红 焦如珍 牟新涛 《林业科学研究》 CSCD 北大核心 2010年第4期530-536,共7页
为了探讨木本豆科植物根瘤菌对不良环境的抵抗能力,对分离自海南尖峰岭国家自然保护区的9株根瘤菌进行了初步研究。结果发现:9株根瘤菌最适生长pH值均为7,最适NaCl含量均为10.0 g.kg-1;菌株CAF224的最适生长温度为37℃,菌株CAF416、CAF... 为了探讨木本豆科植物根瘤菌对不良环境的抵抗能力,对分离自海南尖峰岭国家自然保护区的9株根瘤菌进行了初步研究。结果发现:9株根瘤菌最适生长pH值均为7,最适NaCl含量均为10.0 g.kg-1;菌株CAF224的最适生长温度为37℃,菌株CAF416、CAF438和CAF279的最适温度为20-28℃,其余5株为28℃。9株根瘤菌均可在pH值4-11、NaCl 20.0 g.kg-1、37℃条件下生长,其中CAF224和CAF276菌株耐受pH值3;CAF226可以在40.0 g.kg-1NaCl培养基中生长;CAF276可耐受20 min 50℃、10 min 60℃高温。不同宿主、不同生态条件下的根瘤菌株具有不同的抗逆性,通过综合分析,筛选出4株高抗菌株(CAF226、CAF276、CAF224、CAF414)。16S rDNA序列系统发育分析表明:9菌株在系统进化树上与GenBank中序列号为EF054889的Rhizobium tropiciClone H12(热带根瘤菌)聚为一族,相似度为99.4%,初步确定9株根瘤菌为热带根瘤菌。 展开更多
关键词 尖峰岭 耐盐性试验 pH范围 抗逆性 16S RDNA序列 热带根瘤菌
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Contribution of Promiscuous Soybean (Glycine max L.) to Upland Rice-based Cropping Systems in West Africa 被引量:2
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作者 S. Oikeh P. Houngnandan +3 位作者 A. Robert A. Niang A. Toure B. Kone 《Journal of Agricultural Science and Technology》 2010年第1期54-61,共8页
A two-year on-farm study was carried out at Eglime in the moist savanna (MS) and Ouake in the dry savanna (DS) of Benin to evaluate the contribution of inoculation of dual-purpose soybean varieties to grain yield ... A two-year on-farm study was carried out at Eglime in the moist savanna (MS) and Ouake in the dry savanna (DS) of Benin to evaluate the contribution of inoculation of dual-purpose soybean varieties to grain yield of upland NERICA rice fertilized with low N level. In 2005, four dual-purpose, promiscuous soybean varieties (cv. TGx1440-IE; TG×1448-2E; TG×1019-2EB; and TG×1844-18E), and a popular improved variety (cv. Jupiter) were sown in 12 farmer fields with and without Bradyrhizobium japonicum inoculation. There was also land which was left fallow that acted as the control. In 2006, upland interspecific rice (NERICA 1) was sown in all the plots and supplied with 15 kg N haL. Dry matter yield, N accumulation, and net N-balance were significantly enhanced by over 40% with inoculation of cv. TG× 1844-18E than non-inoculation in the DS in comparison to other cultivars. There were no significant effects of inoculation of previous soybean cultivars on soybean grain yield and on the succeeding NERICA rice yield. Averaged over inoculation, previous cv. TG× 1019-2EB plots supplied with only 15 kg N hal gave the highest grain yield, more than twice the yield of control plots in the DS, possibly because of significant production of higher tillers, panicles and harvest index than the other cultivars; and it could be recommended for upland rice-based system for NERICA production. 展开更多
关键词 BRADYRHIZOBIUM crop rotation degraded savanna promiscuous soybean NERICA rice.
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