热胁迫下月季叶片蛋白双向电泳分析

采用双向聚丙烯酰胺凝胶电泳和肽质量指纹谱,对耐热性不同的月季品种热激处理后进行蛋白质组分析与鉴定。双向电泳后,差异蛋白质点主要集中在分子量10-30kDa、等电点5-6范围内, 对耐热品种在高温下特异表达的蛋白质点中选取3个进行肽质量指纹谱的分析,并检索不同的数据库进行蛋白质鉴定与功能预测,初步认为这些蛋白质点分别是eIF-5A、LEA蛋白和Hsp17．5。同时结合已报道其他植物中这3种蛋白的功能,对月季耐高温生理机制进行初步探讨。

非稳态纳米流体在加磁场平行板间流动的传热分析

本研究采用微分变换法和Akbari-Ganji法,研究了施加均匀磁场对纳米流体在两个无限平行板间流动的自然对流传热的影响。在得到控制方程并在特定边界条件下求解问题后,研究了Prandtl数、挤压次数、Schmidt数、Hartmann数、Eckert数、布朗运动参数和热电泳参数等主要参数的影响。将相似变换用于求解常微分方程组,并与Rung-Kutta四阶数值法进行对比。研究结果表明,增加挤压次数会导致速度减慢,增加Hartman数也有类似的影响。此外,温度随着Hartman数、Eckert数和热电泳参数的增大而升高,且与Prandtl数成正比。我们对比研究了Akbari-Ganji法和微分变换法求解非线性微分方程,结果表明,前者需要的计算步骤更少和计算时间更短,是一种更有效的方法。使用建议的方法获得的解与文献中的解一致。这些结果有助于研究人员更快、更容易地进行分析,并为纳米流体在电磁场存在下流动的复杂行为提供重要见解。

The Nucleosides Contents and Their Variation in Natural Cordyceps sinensis and Cultured Cordyceps Mycelia

Aim: To compare the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia, and to study the effect of humidity and heat on the content of nucleosides. Methods: The contents of nucleosides were determined by using high performance capillary electrophoresis (HPCE). Beckman P/ACE System 5010 apparatus equipped with a UV detector and a Beckman untreated fused-silica capillary (57 cm 75 mm, 50 cm effective length) was used. Before sample injection, the capillary was rinsed with 1 molL-1 sodium hydroxide solution and running buffer for 5 min, respectively. A voltage of 20 kV was applied for the separation. Pressure injection was 586 kPa for 6 seconds, and the wavelength of detector was 254 nm. The running time was 20 min at 20 oC. The effect of humidity and heat on the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia was observed for 1, 3, 5 and 10 days at temperature 40 oC, and relative humidity 75%. Results: The content of nucleosides from natural Cordyceps sinensis was higher than that from cultured Cordyceps mycelia. But the contents of nucleosides from freshly collected natural Cordyceps sinensis were very low, even below the limit of quantitation. The contents of nucleosides from natural Cordyceps sinensis were significantly increased by humidity and heat, but this phenomenon was not observed in cultured Cordyceps mycelia. Conclusion: There are differences between the nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia. The nucleosides in natural Cordyceps sinensis may be derived from the degradation of nucleic acids. This implies that adenosine being used for the quality control of natural Cordyceps sinensis may have to be reconsidered.

Heat Shock Proteins Inducement in Wheat Seedling

High temperature is an important limiting factor to the improvement of wheat yield. When suffered hot stress,wheat will produce a series of heat shock proteins to adapt this adversity. In different upgrowth phases,or different heat-resistant crop varieties,the heat shock protein exist differences. Therefore,study on differences of heat shock protein has significant theoretical and practical meaning for researching heat resistance of wheat. By using SDS-PAGE method,the inducing conditions and manifestation of heat shock protein were studied,which provided theoretical basis for yield increasing of wheat.

Isolation and Characterization of a Thermophilic Oil-Degrading Bacterial Consortium

In this study, a thermophilic oil-degrading bacterial consortium KO8-2 growing within the temperature range of 45--65℃ （with 55℃ being the optimum temperature） was isolated from oil-contaminated soil of Karamay in Xinjiang, China. Denaturing gradient gel electrophoresis （DGGE） showed that there were nine strains included in KO8-2, which originated from the genera of Bacillus, Geobacillus and Clostridium. They all belonged to thermophilic bacteria, and had been previously proved as degraders of at least one petroleum fraction. The crude oil degraded by KO8-2 was analyzed by infrared spectrophotometry, hydrocarbon group type analysis and gas chromatography. The results indicated that the bacterial consortium KO8-2 was able to utilize 64.33% of saturates, 27.06% of aromatics, 13.24% of resins and the oil removal efficiency reached up to 58.73% at 55 ~C when the oil concentration was 10 g/L. Detailed analysis showed that KO8-2 was able to utilize the hydrocarbon components before C19, and the n-alkanes ranging from C20--C33 were signifi- cantly degraded. The ratios of nC17/Pr and nC18/Ph were 3.12 and 3.87, respectively, before degradation, whereas after degradation the ratios reduced to 0.21 and 0.38, respectively. Compared with the control sample, the oil removal efficiency in KO8-2 composting reactor reached 50.12% after a degradation duration of 60 days.

非能动安全壳冷却机制安全壳大气净化分析

采用一体化严重事故分析工具,建立包括主冷却剂系统、专设安全设施、非能动安全壳冷却系统的事故分析模型,该模型耦合了核电厂热工水力、安全壳响应及裂变产物行为分析。研究了小破口失水事故(SB-LOCA)、大破口失水事故(LB-LOCA)以及主给水丧失事故(LOFW)始发严重事故序列下非能动安全壳冷却机制对安全壳大气的净化作用,对非能动安全壳空冷和水冷工况下对安全壳内气溶胶的去除行为进行了分析。通过无冷却、空冷有效、空冷和水冷同时有效的三种工况下气溶胶行为的比对分析,研究了冷却机制对扩散电泳和热电泳引起的气溶胶沉积。分析结果表明,非能动安全壳冷却机制可以增强气溶胶扩散电泳和热电泳的去除效应,相关分析可为严重事故管理导则的制定提供技术支持。