[Objective] The research aimed to reveal the functions of NAC and UBA domains in Peatl＇s thermal stability. [Method] Fusion expression vectors of Pearl protein and the 3 deletion mutants were constructed. The recombi...[Objective] The research aimed to reveal the functions of NAC and UBA domains in Peatl＇s thermal stability. [Method] Fusion expression vectors of Pearl protein and the 3 deletion mutants were constructed. The recombinant plasmids were induced by IPTG and the target proteins （Peatl, Peatl-△CD99,Peatl-△ND49 and Pearl-△ND108 ）were expressed obtained by AKTA and its thermal stability was analyzed. [Result] The research found that 3 deletion mutants have good thermal stability like Pearl. [Conclusion] The research demonstrated that the coexistence of NAC or UBA domains is not necessary to thermal stability of Pearl protein , and they may give the protein particular stability structure seperately.展开更多
[Objective] The aim was to analyse the variation characteristics of temperature in Anqing City and urban heat island effect.[Method] Based on the observation data of temperature from Anqing Station,other surrounding m...[Objective] The aim was to analyse the variation characteristics of temperature in Anqing City and urban heat island effect.[Method] Based on the observation data of temperature from Anqing Station,other surrounding meteorological stations and local automatic meteorological stations in suburbs,the annual variation of temperature and regional consistency was analysed,then the abrupt change of annual average temperature was tested by Mann-Kendall test,finally the influences of urban heat island effect on temperature variation in Anqing Station were studied.[Result] Affected by station migration and urban construction,the annual average temperature increased significantly in Anqing Station from 1977 to 2009,and the rising was more prominent after the middle of the 1990s.Mann-Kendall test showed that the change of temperature in Anqing Station was obviously abrupt around 1993;because of the development of urbanization,average temperature in Anqing Station was 0.8 ℃ higher than that in suburbs,and the minimum temperature rose more remarkably.In addition,urban heat island effect was the strongest in spring,followed by summer and autumn,while it was the weakest in winter.[Conclusion] The effects of urbanization development on the temperature in Anqing City were understood through this research.展开更多
In order to investigate the regulation mechanism of the phycocyanin gene,a series of functional analyses of the upstream sequence of cpcB gene from Arthrospira platensis were conducted in E. coli with green fluorescen...In order to investigate the regulation mechanism of the phycocyanin gene,a series of functional analyses of the upstream sequence of cpcB gene from Arthrospira platensis were conducted in E. coli with green fluorescent protein encoding gene(gfp) as the reporter. Results showed that the gfp gene could express at a high level under the promotion of the upstream sequence,suggesting the existence of some strong promoter elements in it. The expression of GFP was influenced by temperature. Higher temperature led to higher expression level. The bioinformatics analyses followed by mutation analyses on the secondary structure of translation ini-tiation region(TIR) revealed that RNA thermosensor might account for the temperature regulation.展开更多
After infection and integration steps, HIV-1 transcriptions increase sharply and singly-spliced mRNAs are produced. These encode Env （gpl20 and gp41） and auxiliary proteins Vif, Vpr and VpU. The same localization wi...After infection and integration steps, HIV-1 transcriptions increase sharply and singly-spliced mRNAs are produced. These encode Env （gpl20 and gp41） and auxiliary proteins Vif, Vpr and VpU. The same localization within the unique structure of the mRNAs suggests that the VpU sequence prior to the Env could affect the Env polyprotein expression.The HIV-I infection process begins when the gpl20 subunit of the envelope glycoprotein complex interacts with its receptor（s） on the target cell. The V3 domain of gpl20 is the major determinant of cellular co-receptor binding. According to phenotypic information of HIVol isolates, sequences from the VpU to V3 regions （119 in R5- and 120 X4-tropic viruses; one per patient） were analysed. The binomial correlation phi coefficient was used to assess covariation among VpU and gpl20v3 signatures. Subsequently, average linkage hierarchical agglomerative clustering was performed. Beyond the classical V3 signatures （R5-viruses： SI1, E25D; X4-viruses： SllKR, E25KRQ）, other specific V3 and novel VpU signatures were found to be statistically associated with co-receptor usage. Several statistically significant associations between V3 and VpU mutations were also observed. The dendrogram showed two distinct large clusters： one associated with R5-tropic sequences （bootstrap=0.94）, involving： （a） H13NPv3, E25Dv3, Sllv3, T22Av3 and Q61Hvpu, （b） E25Av3 and L12Fvpu, （c） D44Evpu, R18Qv3 and D80Nvpu; and another associated with X4-tropic sequences （bootstrap=0.97）, involving： （i） E25Iv3 and V10Avpu, （ii） 0-1insVvpc, H13Rv3, I46Lvpc, I30Mv3 and 60-62delvpu, （iii） SllKRv3 and E25KRQv3. Some of these pairs of mutations were encoded always by one specific codon. These data indicate the possible VpU mutational patterns contributing to regulation of HIV-I tropism.展开更多
Multiple equilibria and their stability in tropical atmosphere are investigated through β-plane barotropic models with consideration of heating and dissipation. We have derived the solutions of the model equations co...Multiple equilibria and their stability in tropical atmosphere are investigated through β-plane barotropic models with consideration of heating and dissipation. We have derived the solutions of the model equations corresponding to the multiple equilibria or the steady flows first, and then establish the criteria for the stability of steady flow by use of the Liapunov direct Method. When these criteria are applied to the solutions of equilibria obtained, stable flows, which are closely related to the different patterns of quasi-stationary circulation in the tropical region, are lbund. The configurations of these stable flows and the shift between two of them as season changes provide quite reasonable explanations to many fundarnental problems of tropical circulation features such as the catastrophe mechanism of the onset and the break-active cycle of the Asian summer monsoon. It fbllows that the onset or the abrupt transition of the Asian summer monsoon could be attributed to the multiple equilibrium property of the tropical circulation resulted from the advcetive nonlinearity, which provide another explanation among others.展开更多
There is a rainfall variability biennial relationship between Central America （CA） and equatorial South America （ESA） over the tropical western hemisphere, which is known to have arisen due to the combined effects...There is a rainfall variability biennial relationship between Central America （CA） and equatorial South America （ESA） over the tropical western hemisphere, which is known to have arisen due to the combined effects of ENSO and tropical North Atlantic （TNA） SST. Here, the authors report that this biennial rainfall relationship between CA and ESA has weakened remarkably since 2000, with weakening in both in-phase and out-of-phase rainfall transitions. The observed decadal changes in the biennial relationship between CA and ESA rainfall can be attributed to changes in the effects of ENSO and TNA SST since 2000, which may be associated with more frequent occurrences of the central Pacific or＇Modoki＇ type El Ni^o. The weakening of the association with ENSO for CA rainfall since 2000 might have given rise to the weakening of the in-phase rain transition from CA rainfall to the following ESA rainfall. The weakened linkage between boreal-winter ESA rainfall and the subsequent boreal-summer TNA SST since 2000 may have resulted in the weakening of the out- of-phase rainfall transition from boreal-winter ESA rainfall to the subsequent boreal-summer CA rainfall.展开更多
To confirm the existence of point mutations in the SSU rDNA variable regions of 5 Leishmania donovani (L d ) isolates from different epidemic foci in China Methods Specific SSU rDNA fragments from nuclear DNA of 7...To confirm the existence of point mutations in the SSU rDNA variable regions of 5 Leishmania donovani (L d ) isolates from different epidemic foci in China Methods Specific SSU rDNA fragments from nuclear DNA of 7 Leishmania species/isolates were amplified by PCR and then cloned into pGEM R T Easy Vectors After that, the specific fragments were sequenced by an automated DNA sequencer Results Sequence analysis showed that the amplified DNA fragments of 7 Leishmania species/isolates were all 392 bp in length All 5 point mutations were located in two unique sequence blocks (UQ Ⅰ and UQ Ⅱ), and no insertions or deletions were found The identities of comparison of Leishmania in GeneBank were more than 98% Conclusion Five point mutations exist in the SSU rDNA variable region of 5 L d isolates from different epidemic foci of visceral leishmaniasis (VL) in China Sequence differences of the SSU rDNA variable region exist among L d isolates from different foci展开更多
基金Supported by the“973”Program(2003CB114204)the Science and Technology Plan(D0706005040431)~~
文摘[Objective] The research aimed to reveal the functions of NAC and UBA domains in Peatl＇s thermal stability. [Method] Fusion expression vectors of Pearl protein and the 3 deletion mutants were constructed. The recombinant plasmids were induced by IPTG and the target proteins （Peatl, Peatl-△CD99,Peatl-△ND49 and Pearl-△ND108 ）were expressed obtained by AKTA and its thermal stability was analyzed. [Result] The research found that 3 deletion mutants have good thermal stability like Pearl. [Conclusion] The research demonstrated that the coexistence of NAC or UBA domains is not necessary to thermal stability of Pearl protein , and they may give the protein particular stability structure seperately.
文摘[Objective] The aim was to analyse the variation characteristics of temperature in Anqing City and urban heat island effect.[Method] Based on the observation data of temperature from Anqing Station,other surrounding meteorological stations and local automatic meteorological stations in suburbs,the annual variation of temperature and regional consistency was analysed,then the abrupt change of annual average temperature was tested by Mann-Kendall test,finally the influences of urban heat island effect on temperature variation in Anqing Station were studied.[Result] Affected by station migration and urban construction,the annual average temperature increased significantly in Anqing Station from 1977 to 2009,and the rising was more prominent after the middle of the 1990s.Mann-Kendall test showed that the change of temperature in Anqing Station was obviously abrupt around 1993;because of the development of urbanization,average temperature in Anqing Station was 0.8 ℃ higher than that in suburbs,and the minimum temperature rose more remarkably.In addition,urban heat island effect was the strongest in spring,followed by summer and autumn,while it was the weakest in winter.[Conclusion] The effects of urbanization development on the temperature in Anqing City were understood through this research.
基金This work was supported by the National Natural Science Foundation of China （No. 30471317）.
文摘In order to investigate the regulation mechanism of the phycocyanin gene,a series of functional analyses of the upstream sequence of cpcB gene from Arthrospira platensis were conducted in E. coli with green fluorescent protein encoding gene(gfp) as the reporter. Results showed that the gfp gene could express at a high level under the promotion of the upstream sequence,suggesting the existence of some strong promoter elements in it. The expression of GFP was influenced by temperature. Higher temperature led to higher expression level. The bioinformatics analyses followed by mutation analyses on the secondary structure of translation ini-tiation region(TIR) revealed that RNA thermosensor might account for the temperature regulation.
文摘After infection and integration steps, HIV-1 transcriptions increase sharply and singly-spliced mRNAs are produced. These encode Env （gpl20 and gp41） and auxiliary proteins Vif, Vpr and VpU. The same localization within the unique structure of the mRNAs suggests that the VpU sequence prior to the Env could affect the Env polyprotein expression.The HIV-I infection process begins when the gpl20 subunit of the envelope glycoprotein complex interacts with its receptor（s） on the target cell. The V3 domain of gpl20 is the major determinant of cellular co-receptor binding. According to phenotypic information of HIVol isolates, sequences from the VpU to V3 regions （119 in R5- and 120 X4-tropic viruses; one per patient） were analysed. The binomial correlation phi coefficient was used to assess covariation among VpU and gpl20v3 signatures. Subsequently, average linkage hierarchical agglomerative clustering was performed. Beyond the classical V3 signatures （R5-viruses： SI1, E25D; X4-viruses： SllKR, E25KRQ）, other specific V3 and novel VpU signatures were found to be statistically associated with co-receptor usage. Several statistically significant associations between V3 and VpU mutations were also observed. The dendrogram showed two distinct large clusters： one associated with R5-tropic sequences （bootstrap=0.94）, involving： （a） H13NPv3, E25Dv3, Sllv3, T22Av3 and Q61Hvpu, （b） E25Av3 and L12Fvpu, （c） D44Evpu, R18Qv3 and D80Nvpu; and another associated with X4-tropic sequences （bootstrap=0.97）, involving： （i） E25Iv3 and V10Avpu, （ii） 0-1insVvpc, H13Rv3, I46Lvpc, I30Mv3 and 60-62delvpu, （iii） SllKRv3 and E25KRQv3. Some of these pairs of mutations were encoded always by one specific codon. These data indicate the possible VpU mutational patterns contributing to regulation of HIV-I tropism.
基金National Natural Science Foundation of China (40275011 40545019) South China Sea Monsoon Experimentproject
文摘Multiple equilibria and their stability in tropical atmosphere are investigated through β-plane barotropic models with consideration of heating and dissipation. We have derived the solutions of the model equations corresponding to the multiple equilibria or the steady flows first, and then establish the criteria for the stability of steady flow by use of the Liapunov direct Method. When these criteria are applied to the solutions of equilibria obtained, stable flows, which are closely related to the different patterns of quasi-stationary circulation in the tropical region, are lbund. The configurations of these stable flows and the shift between two of them as season changes provide quite reasonable explanations to many fundarnental problems of tropical circulation features such as the catastrophe mechanism of the onset and the break-active cycle of the Asian summer monsoon. It fbllows that the onset or the abrupt transition of the Asian summer monsoon could be attributed to the multiple equilibrium property of the tropical circulation resulted from the advcetive nonlinearity, which provide another explanation among others.
基金funded by the National Natural Science Foundation of China[grant number 41776031]the Guangdong Natural Science Foundation[grant number 2015A030313796]+3 种基金the National Program on Global Change and Air-Sea Interaction[grant number GASI-IPOVAI-04]the Strategic Priority Research Program of the Chinese Academy of Sciences[grant number XDA11010104]the program for scientific research start-upfunds of Guangdong Ocean Universitythe Foundation for Returned Scholars of the Ministry of Education of China
文摘There is a rainfall variability biennial relationship between Central America （CA） and equatorial South America （ESA） over the tropical western hemisphere, which is known to have arisen due to the combined effects of ENSO and tropical North Atlantic （TNA） SST. Here, the authors report that this biennial rainfall relationship between CA and ESA has weakened remarkably since 2000, with weakening in both in-phase and out-of-phase rainfall transitions. The observed decadal changes in the biennial relationship between CA and ESA rainfall can be attributed to changes in the effects of ENSO and TNA SST since 2000, which may be associated with more frequent occurrences of the central Pacific or＇Modoki＇ type El Ni^o. The weakening of the association with ENSO for CA rainfall since 2000 might have given rise to the weakening of the in-phase rain transition from CA rainfall to the following ESA rainfall. The weakened linkage between boreal-winter ESA rainfall and the subsequent boreal-summer TNA SST since 2000 may have resulted in the weakening of the out- of-phase rainfall transition from boreal-winter ESA rainfall to the subsequent boreal-summer CA rainfall.
基金ThisprojectwassupportedbyagrantfromtheNationalNaturalScienceFoundationofChina (No 39970 667)
文摘To confirm the existence of point mutations in the SSU rDNA variable regions of 5 Leishmania donovani (L d ) isolates from different epidemic foci in China Methods Specific SSU rDNA fragments from nuclear DNA of 7 Leishmania species/isolates were amplified by PCR and then cloned into pGEM R T Easy Vectors After that, the specific fragments were sequenced by an automated DNA sequencer Results Sequence analysis showed that the amplified DNA fragments of 7 Leishmania species/isolates were all 392 bp in length All 5 point mutations were located in two unique sequence blocks (UQ Ⅰ and UQ Ⅱ), and no insertions or deletions were found The identities of comparison of Leishmania in GeneBank were more than 98% Conclusion Five point mutations exist in the SSU rDNA variable region of 5 L d isolates from different epidemic foci of visceral leishmaniasis (VL) in China Sequence differences of the SSU rDNA variable region exist among L d isolates from different foci
