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热超声处理对腐败希瓦氏菌可活不可培养状态的影响
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作者 唐泽群 姜紫薇 +3 位作者 邱亮 王露 周婷 廖涛 《广东海洋大学学报》 CAS CSCD 北大核心 2024年第5期163-170,共8页
【目的】研究水产品中常见的腐败希瓦氏菌(Shewanella putrefaciens)在热超声处理下进入可活不可培养(VBNC)状态的生理特性以及复苏状况。【方法】以腐败希瓦氏菌为研究对象,首先采用平板计数法测定腐败希瓦氏菌在不同温度(35、40、45... 【目的】研究水产品中常见的腐败希瓦氏菌(Shewanella putrefaciens)在热超声处理下进入可活不可培养(VBNC)状态的生理特性以及复苏状况。【方法】以腐败希瓦氏菌为研究对象,首先采用平板计数法测定腐败希瓦氏菌在不同温度(35、40、45、50、55、60、65、70℃)和不同功率(100、150、200、250、300 W)热超声处理下的细菌。进而在70℃、不同功率(100、150、200、250、300 W)TS条件下处理腐败希瓦氏菌,利用流式细胞学技术对不同状态的细菌群体进行计数,通过台盼蓝染色验证其存活状态,结合透射电子显微镜观察其形态变化,并使用DNA体外扩增技术检测其VBNC状态特征。最后,将经过70℃、300 W条件TS处理的菌株进行复苏实验。【结果】腐败希瓦氏菌经不同条件的热超声处理后,其细菌死亡率同温度和超声功率成正相关。流式细胞学技术和台盼蓝染色结果表明,在高热超声处理(50 kHz、300 W、超声处理30 min以及70℃加热)下的细菌会大量死亡,但仍有5.87%菌体存活,结合平板计数结果,表明这部分细菌已进入VBNC状态。透射电镜显微镜观察发现,VBNC菌体存在细胞壁和细胞膜变厚,细胞质密度增加,核区模糊的情况。体外扩增结果表明,热超声处理会降解细菌DNA,且复苏实验证明热超声处理后VBNC状态的腐败希瓦氏菌仍存在复苏能力。【结论】在300 W以及70℃加热的热超声处理下会诱导腐败希瓦氏菌进入VBNC状态,处于该状态下的菌体会对TS处理产生一系列生理特性的改变来抵抗外界环境,且当处于适宜环境下时,VBNC状态下的腐败希瓦氏菌会复苏并继续生长增殖。即使经过严格处理过程,仍需对食品安全中的VBNC细菌进行全面监控。 展开更多
关键词 腐败希瓦氏菌 热超声处理 可活不可培养状态 生理特性 细菌复苏
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超声联合热碱技术促进剩余污泥破解的参数优化 被引量:16
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作者 徐慧敏 秦卫华 +1 位作者 何国富 戴晓虎 《中国环境科学》 EI CAS CSSCI CSCD 北大核心 2017年第9期3431-3436,共6页
为了进一步提高超声联合技术破解污泥的效果,在前期双因素的研究基础上,采用3因素3水平的响应曲面法分析了热水解温度(60,70,80°C)、加碱量(0.04,0.07,0.10g/g TS)和超声能量(4000,8000,12000k J/kg TS)对污泥破解度的影响.回归分... 为了进一步提高超声联合技术破解污泥的效果,在前期双因素的研究基础上,采用3因素3水平的响应曲面法分析了热水解温度(60,70,80°C)、加碱量(0.04,0.07,0.10g/g TS)和超声能量(4000,8000,12000k J/kg TS)对污泥破解度的影响.回归分析结果表明污泥破解度与三因素之间符合三元二次方程:Y=-196.87+6.11X_1+487.53X_2+0.0039X_3-1.742X_1X_2-0.000024X_1X_3+0.0044X_2X_3-0.0398X_1~2-2488.33X_2~2-1.33×10-7X_3~2,其中X_1、X_2、X_3分别为温度、加碱量和超声能量.模型拟合度高达97.548%,表明该模型能较好地模拟超声联合热碱预处理参数和预测污泥破解度.根据模型得出最佳预处理工艺组合为:温度73.06℃,加碱量0.085g/g TS,超声能量9551k J/kg TS.最佳工艺参数下污泥破解度实测值为60.411%.经济性分析表明,超声联合热碱预处理条件下每t污泥处理费较未处理污泥降低了20.42元,在本研究条件下具有较高的经济可行性. 展开更多
关键词 响应曲面法 超声联合碱预处理 剩余污泥 经济性
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Damage characteristics of thermally treated granite under uniaxial compression: Insights from active and passive ultrasonic techniques 被引量:2
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作者 GUO Pei WU Shun-chuan +1 位作者 JIANG Ri-hua ZHANG Guang 《Journal of Central South University》 SCIE EI CAS CSCD 2022年第12期4078-4093,共16页
To explore the effects of thermal treatment on cracking processes in granite, granite samples were thermally treated at 25-400 ℃ and then loaded under uniaxial compression. Active ultrasonic testing and passive acous... To explore the effects of thermal treatment on cracking processes in granite, granite samples were thermally treated at 25-400 ℃ and then loaded under uniaxial compression. Active ultrasonic testing and passive acoustic emission(AE) monitoring were combined to monitor the damage characteristics of the samples. The uniaxial compression strength(UCS) of the sample treated at 200 ℃ shows no apparent change compared with that of the nonheated sample, while the UCS increases at 300 °C and decreases at 400 ℃. As the temperature increases from 25 to 400 ℃, the initial P-wave velocity(Vp) decreases gradually from 4909 to 3823 m/s, and the initial Vpanisotropy ε increases slightly from 0.03 to 0.09. As the axial stress increases, ε increases rapidly in the crack closure stage and unstable cracking stage. The attenuation of ultrasonic amplitude spectra also shows an obvious anisotropy. Besides, the main location magnitude of AE events decreases after thermal treatment, and low-frequency AE events and high-amplitude AE events increasingly occur. However, there is insufficient evidence that the treatment temperature below 400 ℃ has a significant effect on the temporal characteristics, source locations, and b-values of AE. 展开更多
关键词 uniaxial compression thermal treatment acoustic emission ULTRASONIC B-VALUE dominant frequency
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Microstructure characteristics and tensile property of ultrasonic treated-thixocast A356 alloy 被引量:4
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作者 Waleed KHALIFA Shimaa EL-HADAD Yoshiki TSUNEKAWA 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2015年第10期3173-3180,共8页
Billets of A356 aluminum alloy were treated using ultrasonic vibrations during solidification.The billets were reheated to the semisolid state at different routes to optimize the procedure.Billets were,then,thixocast ... Billets of A356 aluminum alloy were treated using ultrasonic vibrations during solidification.The billets were reheated to the semisolid state at different routes to optimize the procedure.Billets were,then,thixocast using a die casting machine.The results showed that the ultrasonic-treated billets exhibited finely distributed α(Al) globules after reheating and thixocasting.The thixocast ultrasonic-treated billets showed higher ultimate tensile strength and elongation compared with the untreated billets.Moreover,the thixocast parts showed a tendency to ductile fracture under tension when made from ultrasonic-treated billets,while those made of untreated billets showed brittle fracture with obvious straight facets.These results revealed the feasibility and competence of ultrasonic melt treatment as a potential route for preparing billets for thixocasting. 展开更多
关键词 A356 alloy semi-solid forming ultrasonic treatment REHEATING THIXOCASTING tensile property
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