A filamentous clone ofPolysiphonia urceolata was regenerated from segments cut from the fronds of gametophytes. Unlike wild thalli with short virgate branchlets, the clone was filamentous with few branches. Many trans...A filamentous clone ofPolysiphonia urceolata was regenerated from segments cut from the fronds of gametophytes. Unlike wild thalli with short virgate branchlets, the clone was filamentous with few branches. Many transparent trichoblasts arose from pericentral cells during the induction culture, but these were seldom observed during normal growth. The trichoblasts were uniseriate, often colorless, and formed lobed rhizoids rapidly when they came into contact with solid substrates. In addition to morphological characteristics, the photosynthetic properties and growth conditions of the clone differed from those of the mother plant. Cross-gradient light and temperature culture experiments revealed that the most favorable conditions for culture of the filamentous clone were 22℃ and 95-120 gE/（m2-s） light intensity. The photosynthetic light saturation value for filaments was approx. 100 gE/（m2.s）, which is far lower than that of wild thalli. These results could be used to develop techniques for mass cultures of P. urceolata in photobioreactors for production of seed stock or bioactive products.展开更多
Optical models directly effect the irradiance distribution of observed surface. Traditionally, approximate Lambertian models are widely used in designing the light-emitting diodes (LED) arrays in spite of their errors...Optical models directly effect the irradiance distribution of observed surface. Traditionally, approximate Lambertian models are widely used in designing the light-emitting diodes (LED) arrays in spite of their errors compared with the experimental data. But now a novel LED optical model for uniform illumination system has been proposed, in which the curvefitting technique is used to reduce the inherited errors and modify those previous models. The points from the curve of the LED light intensity are adopted, and a spline curve is designed for fitting, which obtains the revised mode. To verify its feasibility, we apply the new model in a 4×4 array design. The results show that compared with the approximate Lambertian, the light intensity distribution produced by the fitting model is more uniform and intense, as is expected.展开更多
基金Supported by the National Special Research Fund for Non-Profit Sector(No.200905020-06)
文摘A filamentous clone ofPolysiphonia urceolata was regenerated from segments cut from the fronds of gametophytes. Unlike wild thalli with short virgate branchlets, the clone was filamentous with few branches. Many transparent trichoblasts arose from pericentral cells during the induction culture, but these were seldom observed during normal growth. The trichoblasts were uniseriate, often colorless, and formed lobed rhizoids rapidly when they came into contact with solid substrates. In addition to morphological characteristics, the photosynthetic properties and growth conditions of the clone differed from those of the mother plant. Cross-gradient light and temperature culture experiments revealed that the most favorable conditions for culture of the filamentous clone were 22℃ and 95-120 gE/（m2-s） light intensity. The photosynthetic light saturation value for filaments was approx. 100 gE/（m2.s）, which is far lower than that of wild thalli. These results could be used to develop techniques for mass cultures of P. urceolata in photobioreactors for production of seed stock or bioactive products.
基金supported by the National Natural Science Foundation of China (No.60977068)the Science and Technology Key Project of Quanzhou (No.2009G9)
文摘Optical models directly effect the irradiance distribution of observed surface. Traditionally, approximate Lambertian models are widely used in designing the light-emitting diodes (LED) arrays in spite of their errors compared with the experimental data. But now a novel LED optical model for uniform illumination system has been proposed, in which the curvefitting technique is used to reduce the inherited errors and modify those previous models. The points from the curve of the LED light intensity are adopted, and a spline curve is designed for fitting, which obtains the revised mode. To verify its feasibility, we apply the new model in a 4×4 array design. The results show that compared with the approximate Lambertian, the light intensity distribution produced by the fitting model is more uniform and intense, as is expected.
