The envelope fusion protein F of baculoviruses is a class I viral fusion protein which play a significant role during virus entry into insect cells. F is initially synthesized as a precursor（F_0） and then cleaved in...The envelope fusion protein F of baculoviruses is a class I viral fusion protein which play a significant role during virus entry into insect cells. F is initially synthesized as a precursor（F_0） and then cleaved into a disulfide-linked F_1 and F_2 subunits during the process of protein maturation and secretion. To facilitate further investigation into the structure and function of F protein during virus infection, monoclonal antibodies（mAbs） against the F_2 subunit of Helicoverpa armigera nucleopolyhedrovirus（HearNPV）（Ha F） were generated. Two kinds of mAbs were obtained according to their different recognition epitopes： one kind of mAbs, as represented by 38F10,recognizes amino acid（aa） 85 to 123 of F_2 and the other kind, represented by 44D11, recognizes aa148 to 173 of F_2. Western blot and immunofluorescence assay confirmed that both of the mAbs recognized the F protein expressed in HearNPV infected cells, however, only 44D11 could neutralize HearNPV infection. The results further showed that 44D11 may not interact with a receptor binding epitope, rather it was demonstrated to inhibit syncytium formation in cells expressing the Ha F protein. The results imply that the monoclonal antibody 44D11 recognizes a region within HaF_2 that may be involved in the F-mediated membrane fusion process. 基金展开更多
The combination of magnetotelluric survey and laboratory measurements of electrical conductivity is a powerful approach for exploring the conditions of Earth's deep interior. Electrical conductivity of hydrous sil...The combination of magnetotelluric survey and laboratory measurements of electrical conductivity is a powerful approach for exploring the conditions of Earth's deep interior. Electrical conductivity of hydrous silicate melts and aqueous fluids is sensitive to composition, temperature, and pressure, making it useful for understanding partial melting and fluid activity at great depths. This study presents a review on the experimental studies of electrical conductivity of silicate melts and aqueous fluids, and introduces some important applications of experimental results. For silicate melts, electrical conductivity increases with increasing temperature but decreases with pressure. With a similar Na^+ concentration, along the calc-alkaline series electrical conductivity generally increases from basaltic to rhyolitic melt, accompanied by a decreasing activation enthalpy. Electrical conductivity of silicate melts is strongly enhanced with the incorporation of water due to promoted cation mobility. For aqueous fluids, research is focused on dilute electrolyte solutions. Electrical conductivity typically first increases and then decreases with increasing temperature, and increases with pressure before approaching a plateau value. The dissociation constant of electrolyte can be derived from conductivity data. To develop generally applicable quantitative models of electrical conductivity of melt/fluid addressing the dependences on temperature, pressure, and composition, it requires more electrical conductivity measurements of representative systems to be implemented in an extensive P-T range using up-to-date methods.展开更多
基金supported by the grants from the National Science Foundation of China (No. 31370191 and 31621061)the Strategic Priority Research Program of the Chinese Academy of Sciences (grant XDB11030400)Open Research Fund Program of the Key Laboratory of Agricultural and Environmental Microbiology, Chinese Academy of Sciences
文摘The envelope fusion protein F of baculoviruses is a class I viral fusion protein which play a significant role during virus entry into insect cells. F is initially synthesized as a precursor（F_0） and then cleaved into a disulfide-linked F_1 and F_2 subunits during the process of protein maturation and secretion. To facilitate further investigation into the structure and function of F protein during virus infection, monoclonal antibodies（mAbs） against the F_2 subunit of Helicoverpa armigera nucleopolyhedrovirus（HearNPV）（Ha F） were generated. Two kinds of mAbs were obtained according to their different recognition epitopes： one kind of mAbs, as represented by 38F10,recognizes amino acid（aa） 85 to 123 of F_2 and the other kind, represented by 44D11, recognizes aa148 to 173 of F_2. Western blot and immunofluorescence assay confirmed that both of the mAbs recognized the F protein expressed in HearNPV infected cells, however, only 44D11 could neutralize HearNPV infection. The results further showed that 44D11 may not interact with a receptor binding epitope, rather it was demonstrated to inhibit syncytium formation in cells expressing the Ha F protein. The results imply that the monoclonal antibody 44D11 recognizes a region within HaF_2 that may be involved in the F-mediated membrane fusion process. 基金
基金supported by the National Natural Science Foundation of China (Grant Nos. 41402041 & 41322015)the Fundamental Research Funds for the Central Universities of China
文摘The combination of magnetotelluric survey and laboratory measurements of electrical conductivity is a powerful approach for exploring the conditions of Earth's deep interior. Electrical conductivity of hydrous silicate melts and aqueous fluids is sensitive to composition, temperature, and pressure, making it useful for understanding partial melting and fluid activity at great depths. This study presents a review on the experimental studies of electrical conductivity of silicate melts and aqueous fluids, and introduces some important applications of experimental results. For silicate melts, electrical conductivity increases with increasing temperature but decreases with pressure. With a similar Na^+ concentration, along the calc-alkaline series electrical conductivity generally increases from basaltic to rhyolitic melt, accompanied by a decreasing activation enthalpy. Electrical conductivity of silicate melts is strongly enhanced with the incorporation of water due to promoted cation mobility. For aqueous fluids, research is focused on dilute electrolyte solutions. Electrical conductivity typically first increases and then decreases with increasing temperature, and increases with pressure before approaching a plateau value. The dissociation constant of electrolyte can be derived from conductivity data. To develop generally applicable quantitative models of electrical conductivity of melt/fluid addressing the dependences on temperature, pressure, and composition, it requires more electrical conductivity measurements of representative systems to be implemented in an extensive P-T range using up-to-date methods.
