目的检测人卵巢片段化处理及蛋白激酶B刺激物体外对卵泡体外激活的作用.方法人卵巢组织体外行片段化处理,检测Hippo信号通路YES相关蛋白(Yes associated protein,YAP)、磷酸化YAP(p-YAP)及细胞通讯网络(cellular communication network,...目的检测人卵巢片段化处理及蛋白激酶B刺激物体外对卵泡体外激活的作用.方法人卵巢组织体外行片段化处理,检测Hippo信号通路YES相关蛋白(Yes associated protein,YAP)、磷酸化YAP(p-YAP)及细胞通讯网络(cellular communication network,CCN)生长因子、凋亡重复序列的柱状病毒抑制剂(baculoviral inhibitors of apoptosis repeat containing,BIRC)凋亡抑制因子水平,明确片段化处理后对卵巢的影响.将片段化处理后的卵巢组织体外加入AKT刺激物培养后检测磷酸化AKT(p-AKT)表达水平,并进行裸鼠背阔肌移植,探究是否可激活卵泡发育.结果片段化处理降低了人卵巢组织p-YAP表达,增加了CCN生长因子及BIRC凋亡抑制因子的表达.运用AKT刺激物体外培养片段化处理后的卵巢组织p-AKT的表达水平增加,裸鼠背阔肌移植2周后卵巢组织内可见始基卵泡及新生的血管.结论人卵巢组织片段化处理可阻断Hippo信号通路;联合AKT刺激物处理卵巢可以刺激卵泡发育.展开更多
It is important to segment image correctly to extract guidance information for automatic agriculture vehicle. If we can make the computer know where the crops are, we can extract the guidance line easily. Images were ...It is important to segment image correctly to extract guidance information for automatic agriculture vehicle. If we can make the computer know where the crops are, we can extract the guidance line easily. Images were divided into some rec-tangle small windows, then a pair of 1-D arrays was constructed in each small windows. The correlation coefficients of every small window constructed the features to segment images. The results showed that correlation analysis is a potential approach for processing complex farmland for guidance system, and more correlation analysis methods must be researched.展开更多
文摘目的检测人卵巢片段化处理及蛋白激酶B刺激物体外对卵泡体外激活的作用.方法人卵巢组织体外行片段化处理,检测Hippo信号通路YES相关蛋白(Yes associated protein,YAP)、磷酸化YAP(p-YAP)及细胞通讯网络(cellular communication network,CCN)生长因子、凋亡重复序列的柱状病毒抑制剂(baculoviral inhibitors of apoptosis repeat containing,BIRC)凋亡抑制因子水平,明确片段化处理后对卵巢的影响.将片段化处理后的卵巢组织体外加入AKT刺激物培养后检测磷酸化AKT(p-AKT)表达水平,并进行裸鼠背阔肌移植,探究是否可激活卵泡发育.结果片段化处理降低了人卵巢组织p-YAP表达,增加了CCN生长因子及BIRC凋亡抑制因子的表达.运用AKT刺激物体外培养片段化处理后的卵巢组织p-AKT的表达水平增加,裸鼠背阔肌移植2周后卵巢组织内可见始基卵泡及新生的血管.结论人卵巢组织片段化处理可阻断Hippo信号通路;联合AKT刺激物处理卵巢可以刺激卵泡发育.
文摘It is important to segment image correctly to extract guidance information for automatic agriculture vehicle. If we can make the computer know where the crops are, we can extract the guidance line easily. Images were divided into some rec-tangle small windows, then a pair of 1-D arrays was constructed in each small windows. The correlation coefficients of every small window constructed the features to segment images. The results showed that correlation analysis is a potential approach for processing complex farmland for guidance system, and more correlation analysis methods must be researched.