[Objective] This research aimed to optimize continuously the highly efficient regeneration system of Anthurium andraeanum. [Method] The leaves and petioles of four A. andraeanum varieties were used as explants to inve...[Objective] This research aimed to optimize continuously the highly efficient regeneration system of Anthurium andraeanum. [Method] The leaves and petioles of four A. andraeanum varieties were used as explants to investigate the differences in primary callus induction among different A. andraeanum varieties. [Result]The callus formation capacity of SAM and SST was stronger than that of SDM and SHG. Among the four varieties, the leaf regeneration capacity of SAM, SDM and SHG was stronger than the corresponding petiole regeneration capacity. However,the petiole regeneration capacity of SST was stronger. The optimum medium for petiole callus induction of SST was 1/2 MS + TDZ 4.0 mg/L + 2, 4-D 0.2 mg/L with induction rate of 87.5%; the optimum medium for leaf callus induction of SAM was 1/2 MS + TDZ 2.0 mg/L + 2, 4-D 0.2 mg/L with induction rate more than90%; the optimum medium for leaf callus induction of SDM and SHG was all 1/2MS + ZT 2.0 mg/L + 2, 4-D 0.2 mg/L with induction rates of 59.34% and 79.63%,respectively. [Conclusion] In addition to variety differences, the differences in differentiation ability among different types of calluses should be also taken into account in the establishment and optimization of tissue culture and rapid propagation technology system of A. andraeanum.展开更多
[Objective] This study aimed to explore the effects of water and fertilizer regulation on the function of leaves in the filling stage. [Method] Six hybrid rice vari- eties including prematurely aged types and non-prem...[Objective] This study aimed to explore the effects of water and fertilizer regulation on the function of leaves in the filling stage. [Method] Six hybrid rice vari- eties including prematurely aged types and non-prematurely aged ones were selected as experimental materials. Limiting water treatment (with soil water potential of about -25 kpa) and increasing nitrogen treatment (additional 10% of granular nitrogen fertiliz- er in addition to limiting water treatment) were applied after heading to analyse the rates of aging and physiological responses of anti-aging in different hybrid rice vari- eties under water deficit and increased nitrogen conditions taking regular water and fertilizer as control. [Result] The results showed that water deficit accelerated the leaf senescence, and prematurely aged types-'Zhongyou838', 'Tianyou998' and 'Long- ping601 '-were more markedly affected by water deficit, of which the rates of chloro- phyll degradation were 6%-8% higher compared to that in another three hybrids. In- creasing nitrogen treatment raised chlorophyll content and slowed down its degrada- tion. Water deficit caused the increase of abscisic acid (ABA) content to obviously varying degrees in leaves of six hybried rice varieties. Responses of ABA content in six hybried rice varieties to increased nitrogen fertilizer were not consistent. Except in 'Zhongyou838', ABA content in the other five hybrids had varying degrees of in- crease. The responses of the activity of antioxidant enzymes in different hybried rice varieties were inconsistent. In response to increased nitrogen fertilizer in combination with water deficit, the activity of each antioxidant enzyme changed differently. Water deficit enhanced the accumulation of malondialdehyde (MDA) in leaves of each hybrid rice. The correlation analysis showed that chlorophyll content was extremely signifi- cantly positively correlated to the resistance of each antioxidant enzyme; SOD activity in leaves also positively related to ABA content. [Conclusion] The rate of leaf aging, physiological activity of anti-aging, and response to water deficit varied in different hy- bried rice varieties. The activity of antioxidant enzymes were not all induced to improve by ABA. SOD activity can be an indicator of resistance to stress. Increasing nitrogen ap- plication significantly postponed leave senescence in the late growth stage of rice.展开更多
The concentrations of the foliar and surface soil nutrients and the variation with species and stand age were studied inPinus spp. plantations in Zhanggutai area, northeast China. The results showed that the total N, ...The concentrations of the foliar and surface soil nutrients and the variation with species and stand age were studied inPinus spp. plantations in Zhanggutai area, northeast China. The results showed that the total N, total P and C: N ratio of the soil inP. sylvestris var.mongolica stands were significantly higher in comparison with those inP. tabulaeformis andP. densiflora stands. ForP. sylvestris var.mongolica, the foliar P concentration appeared to decrease with age, and the foliar N and K concentrations did not show a consistent change with age. As for the different tree species of the similar age, the foliar N and P concentrations were significantly different (p<0.05), being withP. sylvestris var.mongolica>P. densiflora>P. tabulaeformis. The foliar N: P ratio ofP. densiflora significantly was higher thanP. sylvestris var.mongolica andP. tabulaeformis, while the foliar K was no obvious difference between the three tree species. There were significant correlation (p<0.05) between soil total N and P, soil organic matter and total P, foliar N and P, but it did not show significant correlations between soil and foliar nutrient concentrations, which might attribute to the excessive litter raking, overgrazing and low soil moisture in this area. Based on the foliar N: P ratio, we introduced a combination threshold index of N: P ratio with their absolute foliar nutrient concentrations to determine the possible limiting nutrient. According to the critical N: P ratio and their absolute foliar N, P concentrations, theP. sylvestris var.mongolica stands showed a decreased N limitation degree with age, theP. densiflora stands showed unlimited by N and P in the whole, and theP. tabulaeformis stands showed co-limited by N and P. No significant difference in soil nutrient concentrations of the surface soils was found between 45, 29, 20-yr-oldPinus sylvestris var.mongolica plantation stands. Keywords coniferous trees - foliar nutrient concentration - limiting nutrients - N - P ratio - Zhanggutai sandy land CLC number S718.55 Document code A Article ID 1007-662X(2004)01-0011-08 Foundation item: This research was supported by Key Knowledge Innovation Project (KZCX3-SW-418) of Chinese Academy of Sciences.Biography: CHEN Guang-sheng (1978-), male, master candidate in Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. ChinaResponsible editor: Song Funan展开更多
The N2O emission rates, photosynthesis, respiration and stomatal conductance of the dominant tree species from broadleaf/Korean pine forest in Changbai Mountain were measured by simulated water stress with the closed ...The N2O emission rates, photosynthesis, respiration and stomatal conductance of the dominant tree species from broadleaf/Korean pine forest in Changbai Mountain were measured by simulated water stress with the closed bag-gas chromatography. A total of five species seedlings were involved in this study, i.e.,Pinus koraiensis Sieb. et Zucc,Fraxinus mandshurica Rupr,Juglans mandshurica Maxim,Tilia amurensis Rupr, andQuercus mongolica Fisch. ex Turcz.. The results showed that the stomatal conductance, net photosynthetic rate and N2O emission of leaves were significantly reduced under the water stress. The stoma in the leaves of trees is the main pathway of N2O emission. N2O emission in the trees mainly occurred during daytime. N2O emission rates were different in various tree specie seedlings at the same water status. In the same tree species, N2O emission rates decreased as the reduction of soil water contents. At different soil water contents (MW, LW) the N2O emission rates ofPinus koraiensis decreased by 34.43% and 100.6% of those in normal water condition, respectively. In broadleaf arbor decreased by 31.93% and 86.35%, respectively. Under different water stresses N2O emission rates in five tree species such asPinus koraiensis, Fraxinus mandshurica, Juglans mandshurica, Tilia amurensis, andQuercus mongolica were 38.22, 14.44, 33.02, 16.48 and 32.33 ngN2O·g?1DW·h?1, respectively. Keywords Trees - N2O emission rate - Soil water stress - broadleaf/Korean pine forest - Changbai Mountain CLC number S718.55 Document code A Foundation item: This project was supported by the National Natural Science Foundation of China (No. 30271068), the grant of the Knowledge Innovation Program of Chinese Academy of Sciences (KZ-CX-SW-01-01B-10), and the Special Funds for Major State Basic Research Program of China (No. G1999043407)Biography: Wang Miao (1964-), male, associate professor in Institute of Applied Ecology, Chinese Academy of Science, Shenyang 110016, P. R. China.Responsible editor: Song Funan展开更多
Three winter wheat cultivars ( Triticum aestivum L.), representatives of those widely cultivated in Beijing over the past six decades, were grown in the same environmental condition, and their physiological features w...Three winter wheat cultivars ( Triticum aestivum L.), representatives of those widely cultivated in Beijing over the past six decades, were grown in the same environmental condition, and their physiological features were investigated. Daily changes of net photosynthetic rate (P-n), transpiration (T-r) in different growth stages were measured in order to find the relationship between leaf photosynthesis and yield. Instantaneous water use efficiency (WUE) of leaf was calculated from P-n/T-r. It is suggested that relationship between photosynthetic rate and yield changed with the developing stages of wheat. High yield wheat cultivar Jingdong 8 (released in the 1990s) had a higher photosynthetic rate ( the maximal P-n increased by 77%) and transpiration rate (the maximal T-r increased by 69%), but a lower WUE than the low yield cultivar Yanda 1817 (released in the 1940s) during the day time at stem elongation stage. However; difference of P-n among the three cultivars changed with wheat growth process. Before 10 o'clock P-n in leaves of Jingdong 8 usually was the highest of the three cultivars, but P-n of Yanda 1817 was the highest after 10 o'clock. At dough ripe stage, P-n in leaves of Yanda. 1817 was the highest among the three cultivars during the whole day. The difference of changing trend of transpiration in three wheat cultivars was similar to P,, but WUE of Yanda 1817 was the highest in those three cultivars, indicating that the higher yield of Jingdong 8 was achieved via a greater consumption of water. Contrary to the cultivars released in the later period, midday depression of photosynthesis was small in Yanda 1817, which might suggest that Yanda 1817 was resistant to photoinhibition. It is possible that photosynthetic potential in leaves of wheat increased as wheat cultivars was improved over the past six decades. However, it became less resistant to photoinhibition.展开更多
文摘[Objective] This research aimed to optimize continuously the highly efficient regeneration system of Anthurium andraeanum. [Method] The leaves and petioles of four A. andraeanum varieties were used as explants to investigate the differences in primary callus induction among different A. andraeanum varieties. [Result]The callus formation capacity of SAM and SST was stronger than that of SDM and SHG. Among the four varieties, the leaf regeneration capacity of SAM, SDM and SHG was stronger than the corresponding petiole regeneration capacity. However,the petiole regeneration capacity of SST was stronger. The optimum medium for petiole callus induction of SST was 1/2 MS + TDZ 4.0 mg/L + 2, 4-D 0.2 mg/L with induction rate of 87.5%; the optimum medium for leaf callus induction of SAM was 1/2 MS + TDZ 2.0 mg/L + 2, 4-D 0.2 mg/L with induction rate more than90%; the optimum medium for leaf callus induction of SDM and SHG was all 1/2MS + ZT 2.0 mg/L + 2, 4-D 0.2 mg/L with induction rates of 59.34% and 79.63%,respectively. [Conclusion] In addition to variety differences, the differences in differentiation ability among different types of calluses should be also taken into account in the establishment and optimization of tissue culture and rapid propagation technology system of A. andraeanum.
基金Supported by the National Natural Science Foundation of China(30860137)the Research Fund of Department of Education of Jiangxi Province(GJJ09168)~~
文摘[Objective] This study aimed to explore the effects of water and fertilizer regulation on the function of leaves in the filling stage. [Method] Six hybrid rice vari- eties including prematurely aged types and non-prematurely aged ones were selected as experimental materials. Limiting water treatment (with soil water potential of about -25 kpa) and increasing nitrogen treatment (additional 10% of granular nitrogen fertiliz- er in addition to limiting water treatment) were applied after heading to analyse the rates of aging and physiological responses of anti-aging in different hybrid rice vari- eties under water deficit and increased nitrogen conditions taking regular water and fertilizer as control. [Result] The results showed that water deficit accelerated the leaf senescence, and prematurely aged types-'Zhongyou838', 'Tianyou998' and 'Long- ping601 '-were more markedly affected by water deficit, of which the rates of chloro- phyll degradation were 6%-8% higher compared to that in another three hybrids. In- creasing nitrogen treatment raised chlorophyll content and slowed down its degrada- tion. Water deficit caused the increase of abscisic acid (ABA) content to obviously varying degrees in leaves of six hybried rice varieties. Responses of ABA content in six hybried rice varieties to increased nitrogen fertilizer were not consistent. Except in 'Zhongyou838', ABA content in the other five hybrids had varying degrees of in- crease. The responses of the activity of antioxidant enzymes in different hybried rice varieties were inconsistent. In response to increased nitrogen fertilizer in combination with water deficit, the activity of each antioxidant enzyme changed differently. Water deficit enhanced the accumulation of malondialdehyde (MDA) in leaves of each hybrid rice. The correlation analysis showed that chlorophyll content was extremely signifi- cantly positively correlated to the resistance of each antioxidant enzyme; SOD activity in leaves also positively related to ABA content. [Conclusion] The rate of leaf aging, physiological activity of anti-aging, and response to water deficit varied in different hy- bried rice varieties. The activity of antioxidant enzymes were not all induced to improve by ABA. SOD activity can be an indicator of resistance to stress. Increasing nitrogen ap- plication significantly postponed leave senescence in the late growth stage of rice.
基金Key Knowledge Innovation Project (KZCX3-SW-418) of Chinese Academy of Sciences.
文摘The concentrations of the foliar and surface soil nutrients and the variation with species and stand age were studied inPinus spp. plantations in Zhanggutai area, northeast China. The results showed that the total N, total P and C: N ratio of the soil inP. sylvestris var.mongolica stands were significantly higher in comparison with those inP. tabulaeformis andP. densiflora stands. ForP. sylvestris var.mongolica, the foliar P concentration appeared to decrease with age, and the foliar N and K concentrations did not show a consistent change with age. As for the different tree species of the similar age, the foliar N and P concentrations were significantly different (p<0.05), being withP. sylvestris var.mongolica>P. densiflora>P. tabulaeformis. The foliar N: P ratio ofP. densiflora significantly was higher thanP. sylvestris var.mongolica andP. tabulaeformis, while the foliar K was no obvious difference between the three tree species. There were significant correlation (p<0.05) between soil total N and P, soil organic matter and total P, foliar N and P, but it did not show significant correlations between soil and foliar nutrient concentrations, which might attribute to the excessive litter raking, overgrazing and low soil moisture in this area. Based on the foliar N: P ratio, we introduced a combination threshold index of N: P ratio with their absolute foliar nutrient concentrations to determine the possible limiting nutrient. According to the critical N: P ratio and their absolute foliar N, P concentrations, theP. sylvestris var.mongolica stands showed a decreased N limitation degree with age, theP. densiflora stands showed unlimited by N and P in the whole, and theP. tabulaeformis stands showed co-limited by N and P. No significant difference in soil nutrient concentrations of the surface soils was found between 45, 29, 20-yr-oldPinus sylvestris var.mongolica plantation stands. Keywords coniferous trees - foliar nutrient concentration - limiting nutrients - N - P ratio - Zhanggutai sandy land CLC number S718.55 Document code A Article ID 1007-662X(2004)01-0011-08 Foundation item: This research was supported by Key Knowledge Innovation Project (KZCX3-SW-418) of Chinese Academy of Sciences.Biography: CHEN Guang-sheng (1978-), male, master candidate in Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. ChinaResponsible editor: Song Funan
基金The National Natural Science Foundation of China (No. 30271068) the grant of the Knowledge Inno-vation Program of Chinese Academy of Sciences (KZ-CX-SW-01-01B-10) and the Special Funds for Major State Basic Research Pr
文摘The N2O emission rates, photosynthesis, respiration and stomatal conductance of the dominant tree species from broadleaf/Korean pine forest in Changbai Mountain were measured by simulated water stress with the closed bag-gas chromatography. A total of five species seedlings were involved in this study, i.e.,Pinus koraiensis Sieb. et Zucc,Fraxinus mandshurica Rupr,Juglans mandshurica Maxim,Tilia amurensis Rupr, andQuercus mongolica Fisch. ex Turcz.. The results showed that the stomatal conductance, net photosynthetic rate and N2O emission of leaves were significantly reduced under the water stress. The stoma in the leaves of trees is the main pathway of N2O emission. N2O emission in the trees mainly occurred during daytime. N2O emission rates were different in various tree specie seedlings at the same water status. In the same tree species, N2O emission rates decreased as the reduction of soil water contents. At different soil water contents (MW, LW) the N2O emission rates ofPinus koraiensis decreased by 34.43% and 100.6% of those in normal water condition, respectively. In broadleaf arbor decreased by 31.93% and 86.35%, respectively. Under different water stresses N2O emission rates in five tree species such asPinus koraiensis, Fraxinus mandshurica, Juglans mandshurica, Tilia amurensis, andQuercus mongolica were 38.22, 14.44, 33.02, 16.48 and 32.33 ngN2O·g?1DW·h?1, respectively. Keywords Trees - N2O emission rate - Soil water stress - broadleaf/Korean pine forest - Changbai Mountain CLC number S718.55 Document code A Foundation item: This project was supported by the National Natural Science Foundation of China (No. 30271068), the grant of the Knowledge Innovation Program of Chinese Academy of Sciences (KZ-CX-SW-01-01B-10), and the Special Funds for Major State Basic Research Program of China (No. G1999043407)Biography: Wang Miao (1964-), male, associate professor in Institute of Applied Ecology, Chinese Academy of Science, Shenyang 110016, P. R. China.Responsible editor: Song Funan
文摘Three winter wheat cultivars ( Triticum aestivum L.), representatives of those widely cultivated in Beijing over the past six decades, were grown in the same environmental condition, and their physiological features were investigated. Daily changes of net photosynthetic rate (P-n), transpiration (T-r) in different growth stages were measured in order to find the relationship between leaf photosynthesis and yield. Instantaneous water use efficiency (WUE) of leaf was calculated from P-n/T-r. It is suggested that relationship between photosynthetic rate and yield changed with the developing stages of wheat. High yield wheat cultivar Jingdong 8 (released in the 1990s) had a higher photosynthetic rate ( the maximal P-n increased by 77%) and transpiration rate (the maximal T-r increased by 69%), but a lower WUE than the low yield cultivar Yanda 1817 (released in the 1940s) during the day time at stem elongation stage. However; difference of P-n among the three cultivars changed with wheat growth process. Before 10 o'clock P-n in leaves of Jingdong 8 usually was the highest of the three cultivars, but P-n of Yanda 1817 was the highest after 10 o'clock. At dough ripe stage, P-n in leaves of Yanda. 1817 was the highest among the three cultivars during the whole day. The difference of changing trend of transpiration in three wheat cultivars was similar to P,, but WUE of Yanda 1817 was the highest in those three cultivars, indicating that the higher yield of Jingdong 8 was achieved via a greater consumption of water. Contrary to the cultivars released in the later period, midday depression of photosynthesis was small in Yanda 1817, which might suggest that Yanda 1817 was resistant to photoinhibition. It is possible that photosynthetic potential in leaves of wheat increased as wheat cultivars was improved over the past six decades. However, it became less resistant to photoinhibition.