A method of high-performance liquid chromatography(HPLC)was established for simultaneous determination of 27 preservatives in toothpaste.Toothpaste samples were extracted with 50%aqueous methanol solution,ultrasonicat...A method of high-performance liquid chromatography(HPLC)was established for simultaneous determination of 27 preservatives in toothpaste.Toothpaste samples were extracted with 50%aqueous methanol solution,ultrasonicated and then filtered.The target compounds were separated on a C_(18) column(250 mm×4.6μm,5μm)with the mobile phase consisting of acetonitrile and aqueous phosphoric acid solution,the column temperature was 30℃,the sample volume was 10μL,the flow rate was 1 mL/min,and diode array detector was used.Under the optimal experimental conditions,good linear relationship was obtained and the correlation coefficient(R^(2))was greater than 0.9995.This method was accurate and sensitive,which was suitable for the qualitative and quantitative analysis of 27 preservatives in toothpaste.展开更多
文摘A method of high-performance liquid chromatography(HPLC)was established for simultaneous determination of 27 preservatives in toothpaste.Toothpaste samples were extracted with 50%aqueous methanol solution,ultrasonicated and then filtered.The target compounds were separated on a C_(18) column(250 mm×4.6μm,5μm)with the mobile phase consisting of acetonitrile and aqueous phosphoric acid solution,the column temperature was 30℃,the sample volume was 10μL,the flow rate was 1 mL/min,and diode array detector was used.Under the optimal experimental conditions,good linear relationship was obtained and the correlation coefficient(R^(2))was greater than 0.9995.This method was accurate and sensitive,which was suitable for the qualitative and quantitative analysis of 27 preservatives in toothpaste.
