目的:探讨人脱落乳牙牙髓干细胞(stem cells from human exfoliated deciduous teeth,SHED)在牙槽骨缺损修复中的作用机制,利用Molday ION Rhodamine B (MIRB)标记示踪SHED在牙槽骨缺损修复中的归转。方法:利用MIRB标记SHED,检测细胞的...目的:探讨人脱落乳牙牙髓干细胞(stem cells from human exfoliated deciduous teeth,SHED)在牙槽骨缺损修复中的作用机制,利用Molday ION Rhodamine B (MIRB)标记示踪SHED在牙槽骨缺损修复中的归转。方法:利用MIRB标记SHED,检测细胞的标记效率及标记后细胞的存活、增殖及成骨分化能力。通过核磁影像、免疫组织化学-荧光共染色双模态示踪及H-E染色,分析MIRB标记的SHED移植到大鼠牙槽骨缺损模型后,在体内的存活、分化及促进损伤的牙槽骨组织愈合情况。采用SPSS 24.0软件包对数据进行统计学分析。结果:MIRB标记SHED不影响其生长及成骨分化的最佳标记浓度为25μg/mL,对SHED的标记效率达到100%。通过核磁成像示踪体内移植MIRB标记的SHED,可在体内存活8周以上,MIRB标记的SHED能够在体内分化成骨细胞且明显促进牙槽骨的缺损修复。结论:MIRB标记的SHED可以进行体内示踪,并观察到标记的SHED促进缺损牙槽骨修复。展开更多
OBJECTIVE: To study the application of dental implant distractor (DID) in mandibular functional reconstruction. METHODS: We designed a new device named DID, which includes the permanent dental implant and the temporar...OBJECTIVE: To study the application of dental implant distractor (DID) in mandibular functional reconstruction. METHODS: We designed a new device named DID, which includes the permanent dental implant and the temporary distractor in itself. It is specially designed for fibula wider distraction in mandible reconstruction. Twenty-five sets of DID devices were put into 8 patients (6 men and 2 women) during operation. Two patients suffered from ameloblastoma of the mandible, 2 from odontogenic cyst of the mandible, 1 from fibrous dysplasia, and the other 3 from malignant tumor of the mandible. The age of 8 patients ranged from 19 to 67 (mean 46.8) years. RESULTS: During postoperative 2 - 15 months follow up, 7 patients were found to be successful. The clinical examination and X-ray film showed the normal shape of the mandible and the osteointegration of the implants were solid enough to withstand the denture force. Others had the DID removed because of inflammation. Two of the patients successfully worn the fixed dental prosthesis. The outcomes was satisfying. CONCLUSION: The DID device specially designed for mandibular reconstruction with fibular flap can help to simplify convenient procedures to a single surgery.展开更多
文摘目的:探讨人脱落乳牙牙髓干细胞(stem cells from human exfoliated deciduous teeth,SHED)在牙槽骨缺损修复中的作用机制,利用Molday ION Rhodamine B (MIRB)标记示踪SHED在牙槽骨缺损修复中的归转。方法:利用MIRB标记SHED,检测细胞的标记效率及标记后细胞的存活、增殖及成骨分化能力。通过核磁影像、免疫组织化学-荧光共染色双模态示踪及H-E染色,分析MIRB标记的SHED移植到大鼠牙槽骨缺损模型后,在体内的存活、分化及促进损伤的牙槽骨组织愈合情况。采用SPSS 24.0软件包对数据进行统计学分析。结果:MIRB标记SHED不影响其生长及成骨分化的最佳标记浓度为25μg/mL,对SHED的标记效率达到100%。通过核磁成像示踪体内移植MIRB标记的SHED,可在体内存活8周以上,MIRB标记的SHED能够在体内分化成骨细胞且明显促进牙槽骨的缺损修复。结论:MIRB标记的SHED可以进行体内示踪,并观察到标记的SHED促进缺损牙槽骨修复。
文摘OBJECTIVE: To study the application of dental implant distractor (DID) in mandibular functional reconstruction. METHODS: We designed a new device named DID, which includes the permanent dental implant and the temporary distractor in itself. It is specially designed for fibula wider distraction in mandible reconstruction. Twenty-five sets of DID devices were put into 8 patients (6 men and 2 women) during operation. Two patients suffered from ameloblastoma of the mandible, 2 from odontogenic cyst of the mandible, 1 from fibrous dysplasia, and the other 3 from malignant tumor of the mandible. The age of 8 patients ranged from 19 to 67 (mean 46.8) years. RESULTS: During postoperative 2 - 15 months follow up, 7 patients were found to be successful. The clinical examination and X-ray film showed the normal shape of the mandible and the osteointegration of the implants were solid enough to withstand the denture force. Others had the DID removed because of inflammation. Two of the patients successfully worn the fixed dental prosthesis. The outcomes was satisfying. CONCLUSION: The DID device specially designed for mandibular reconstruction with fibular flap can help to simplify convenient procedures to a single surgery.
