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牛体内囊胚全转录组模式解析 被引量:4
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作者 王晶晶 张培培 +7 位作者 郝海生 杜卫华 庞云渭 权国波 赵善江 邹惠影 朱化彬 赵学明 《畜牧兽医学报》 CAS CSCD 北大核心 2020年第6期1271-1280,共10页
旨在解析牛体内囊胚的全转录组模式。本研究选择3头15月龄以上、健康状况良好的泌乳奶牛,采用超排获得体内囊胚,采用单细胞全转录组测序技术检测囊胚mRNA、lncRNA、circRNA转录模式,并采用生物信息学工具对其进行分析。试验获得牛体内... 旨在解析牛体内囊胚的全转录组模式。本研究选择3头15月龄以上、健康状况良好的泌乳奶牛,采用超排获得体内囊胚,采用单细胞全转录组测序技术检测囊胚mRNA、lncRNA、circRNA转录模式,并采用生物信息学工具对其进行分析。试验获得牛体内囊胚约19975个mRNAs、12715个novel lncRNAs及887个circRNAs。mRNA有12种可变剪切方法,以转录起始区域可变剪切(TSS)为主。采用基因本体(GO)和相关信号通路(KEGG)对其分析发现,生物功能主要富集在新陈代谢、细胞膜和蛋白结合、细胞通讯、细胞组织成分、生长发育等功能,Pathway主要富集在细胞周期、膜运输、染色体组织调控等通路;circRNA有6种类型,其中CLASSIC类型circRNA数量最多,这种剪切方式会形成成环的外显子circRNA(EcircRNA)。本研究阐明了牛体内囊胚的全转录组模式,为全面了解牛体内囊胚提供了新的思路。 展开更多
关键词 牛体内囊胚 MRNA lncRNA circRNA
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基于单细胞转录组测序技术筛选牛体内囊胚性别特异性基因
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作者 崔宝山 黄飞 +2 位作者 王杰 李楠 高庆华 《中国畜牧兽医》 CAS 北大核心 2022年第12期1473-1481,共9页
【目的】探究牛体内雌性和雄性囊胚在mRNA水平上的特异性差异,挖掘雌性和雄性囊胚发育差异相关候选基因。【方法】参考GenBank中牛牙釉质基因(AMEL)序列(AMELX基因,登录号:NM_001014984.1;AMELY基因,登录号:NM_174240.2)设计引物,以胚... 【目的】探究牛体内雌性和雄性囊胚在mRNA水平上的特异性差异,挖掘雌性和雄性囊胚发育差异相关候选基因。【方法】参考GenBank中牛牙釉质基因(AMEL)序列(AMELX基因,登录号:NM_001014984.1;AMELY基因,登录号:NM_174240.2)设计引物,以胚胎内细胞DNA为模板,采用巢式PCR扩增技术鉴定单个牛体内囊胚的性别。选用确定性别的单个雌性和雄性囊胚为试验材料,采用Smart-Seq2扩增技术构建单个胚胎测序文库,应用Illumina HiSeq Xten高通量测序平台对单个胚胎进行单细胞转录组测序(scRNA-Seq),并进行了基因差异表达、GO功能和KEGG通路分析。【结果】通过巢式PCR扩增胚胎内细胞中的AMEL基因,确定了胚胎性别;基因表达分析筛选出两组之间差异表达基因(DEGs)6 160个,其中雌性特异性基因675个,雄性特异性基因305个;GO功能注释发现雌性特异性基因显著注释在核苷酸结合、信号转导调控、多细胞生物发育、细胞骨架等条目,雄性特异性基因显著注释在氧化磷酸化、线粒体、线粒体内膜、核糖体等条目;KEGG通路富集分析发现7条与雌性和雄性囊胚发育差异相关的通路,分别为代谢途径、糖酵解、磷酸戊糖途径、细胞衰老、氧化磷酸化、调节干细胞多能性的信号通路和Wnt信号通路;并利用GO和KEGG结果筛选出5个在牛体内囊胚期胚胎发育过程中具有直接或间接作用的基因:FBP1、GADD45G、FHL2、FOSB和WNT2B。【结论】牛体内雌性和雄性囊胚之间存在广泛的转录差异,性别特异性基因FBP1、GADD45G、FHL2、FOSB和WNT2B可能是直接或间接影响胚胎发育的关键基因。 展开更多
关键词 牛体内囊胚 单细胞转录组测序(scRNA-Seq) 性别特异性基因 富集分析
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阿维菌素驱杀牛体内外寄生虫的对比试验 被引量:8
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作者 陈大林 鲜升文 +1 位作者 谭永孝 周宝 《中国兽医科技》 CSCD 2000年第6期33-34,共2页
关键词 牛体内寄生虫病 体外寄生虫病 阿维菌素 驱虫
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牛体内寄生虫病的防治
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作者 张志霞 《畜牧兽医科技信息》 2016年第6期74-74,共1页
1球虫病1.1临床症状犊牛全天精神欠佳,消瘦,体温略高,发病到了中期出现了温度升高至40℃以上,肠胃状况不佳,到了后期,贫血,衰弱,粪便呈现黑色。1.2治疗治疗采取氨丙林,1次/d,连用5d以上,莫能菌素按30mg/kg添加,同时也可结合止痢,补液等... 1球虫病1.1临床症状犊牛全天精神欠佳,消瘦,体温略高,发病到了中期出现了温度升高至40℃以上,肠胃状况不佳,到了后期,贫血,衰弱,粪便呈现黑色。1.2治疗治疗采取氨丙林,1次/d,连用5d以上,莫能菌素按30mg/kg添加,同时也可结合止痢,补液等辅助治疗。2焦虫病2.1临床症状病牛迅速消瘦,贫血,排尿困难。发病初期可以体温升至40℃以上,脉搏和呼吸不断加快,精神方面异常, 展开更多
关键词 牛体内 寄生虫 预防 策略
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Energy Value and Amino Acid Bioavailability of Faba Bean Seeds in Broilers Diets
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作者 Dimitrios Gourdouvelis Vassilios Dotas +3 位作者 Ioannis Nikolakakis Konstantinos Papanikolaou LambrosHatzizisis Dimitrios Dotas 《Journal of Agricultural Science and Technology(A)》 2012年第2期161-167,共7页
The aim of this study was to determine the apparent (AME) and true metabolizable energy (TMEn) as well as the crude protein (CP) and amino acid (AA) total tract (by excreta collection) digestibility (bioava... The aim of this study was to determine the apparent (AME) and true metabolizable energy (TMEn) as well as the crude protein (CP) and amino acid (AA) total tract (by excreta collection) digestibility (bioavailability) of faba bean seeds (FBS) of the Greek cultivar "Polikarpi". Forty eight broilers were placed in individual cages and randomly allocated into 4 dietary treatments. Birds consumed 80 g d1 of either a typical commercial diet or the same diet in which 100, 200 or 300 g kg^-1 had been substituted by ground FBS. The experiment lasted 15 d. Apparent and true CP bioavailability of FBS were significantly decreased (P 〈 0.05) only at the inclusion rate of 300 g kg-1. AA bioavailability remained at high levels (-0.80) with the exception of cystine and was similar to CP mean. The mean AME and TMEn values of FBS were estimated equal to 11.1 and 11.4 MJ ME kg^-1, respectively. 展开更多
关键词 Faba bean seeds BROILERS metabolizable enemy crude protein amino acid bioavailabilitv.
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Introduction to the Turbulent Flows Theory--An Axially-Symmetric Non-peaceful Flows
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作者 Tomasz M. Jankowski 《Journal of Mechanics Engineering and Automation》 2014年第4期327-357,共31页
In fluid mechanics, there is lack of turbulent flows theory. The theory of laminar flows is also not completed. Therefore, the aim of this paper is to fill in the gaps in the theory of laminar flow. First time in the ... In fluid mechanics, there is lack of turbulent flows theory. The theory of laminar flows is also not completed. Therefore, the aim of this paper is to fill in the gaps in the theory of laminar flow. First time in the history of fluid mechanics, it shows that the value of the boundary Reynolds number, dividing the flows of Newtonian fluids into laminar and turbulent, can be calculated theoretically. The very existence of this boundary has been known to researchers over a century ago, but all knowledge in this area so far comes only from experimental physics. This paper marks a turning point, as it not only explains the theory of the said phenomenon, but also presents the methodology of its analytical description. It should be noted that the said description refers to the type of flow which is best understood by scientists, i.e., steady axially-symmetric laminar flow through a straight-axis duct of a circular cross-section of the radius R under the action of constant natural gravitational forces. This paper comes second in a series of papers aimed at eliminating chaos from fluid mechanics and replacing it with harmony and mathematical order. 展开更多
关键词 Dualism theory primary motion secondary motion.
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