Physicochemical characteristics of various milk samples available in Pakistan

We report physicochemical characteristics of various kinds of liquid milk commercially available in Pakistan in comparison with those of fresh natural milk from animals. Milk samples were collected from local markets at Peshawar, Pakistan, and analyzed for their physical features, including moisture, total solids, specific gravity, conductivity, viscosity and titratable acidity (lactic acid equivalent), and chemical components and macro-minerals, including total protein, casein, lactose, ash and minerals (Na, K and Mg). These items were compared with the physicochemical characteristics of the fresh natural milk samples from buffalo, cow and goat. The results were also compared with reported nutritional quality of milk from various countries and World Health Organization (WHO) standards. We found that all the physical features and chemical components of commercially available milk in Pakistan markets meet WHO's requirements, except for Na, K, Ca and Mg, which are below the standards.

Characterization of Microbial Community Structure in Rhizosphere Soils of Cowskin Azalea(Rhododendron aureum Georgi)on Northern Slope of Changbai Mountains,China

The vegetation and soil are mutual environmental factors, soil characteristics, such as chemical properties and microorganism that affect the vegetation occurrence, development and succession speed. In this study, we evaluated the structure of microbial communities of rhizosphere of Cowskin Azalea(Rhododendron aureum Georgi) populations and compared with non-rhizosphere soils at four sample sites of the Changbai Mountains, China, and analyzed the correlation between chemical properties of soil and microbial communities. The results showed that microbial structure and soil chemical properties are significant superior to non-rhizosphere at all four sample sites. The rhizosphere microorganisms are mainly composed of bacteria, actinomycetes, followed by fungi least. The principal component analysis(PCA) biplot displayed that there are differences between rhizosphere and non-rhizosphere soils for microflora; Through correlation analysis, we found that the bacteria is clearly influenced by p H on the Changbai Mountains, besides p H, other soil features such as NO3–-N. These data highlight that R. aureum as the dominant vegetation living in the alpine tundra is a key factor in the formation of soil microorganism and improving soil fertility, and is of great significance for the maintenance of alpine tundra ecosystem.

Molecular and in vitro Characterization of Field Isolates of Bovine Herpesvirus-1

Bovine Herpesvirus-1 (BoHV-1) is distributed worldwide and is a major pathogen in cattle, being the causal agent of a variety of clinical syndromes. The aim of this study was to isolate and to characterize (molecular and biological characterization) BoHV-1 from 29 immunosuppressed animals. It was possible to obtain 18 isolates, each from a different animal, such as from the respiratory and reproductive tracts. In some cases the cytopathic effect was visible 12 hours post-inoculation, and became characteristic after 36-48 hours. Biological characteristics were evaluated and compared with Iowa and Colorado-1 reference strains, and differences were found in plaque size, virus titer measured by TCID50 and PFU/mL, and one step virus curves. These results showed that some isolates had a highly virulent-like behavior in vitro, compared to the reference strains, with shorter eclipse periods, faster release of virus into the supernatants, and higher burst size and viral titer. There were no differences in glycoprotein expression of BoHV-1 isolates, measured by Western blot on monolayers. Moreover, using restriction endonucleases analysis, most of the viruses were confirmed as BoHV-1.1 and just one of them was confirmed as BoHV-1.2a subtype. These findings suggest that some wild-type BoHV-1 isolates could be useful as seeds to develop new monovalent vaccines.

Characterization of Bovine and Ovine WPC Obtained by Different Membrane Configuration Processes

The objective of this stud）, was to valorize bovine and ovine cheese whey resulting from small and medium cheese manufacture plants, by producing liquid and dr）＂ whey protein concentrates （LWPC and WPC）. The flexibility implemented by batch ultrafiltration （UF） and diafiltration （DF） allowed for the production and ovine WPC with 61 -87% （dry basis）. Diatiltration, performed in of liquid bovine WPC with 43-66% protein contents （dry basis） sequential dilution mode （DFsdm） did not improve significantly the composition of WPC liquid products comparing to the results achieved by conventional UF. However, using DF in volume reduction mode （DFvrm） protein content was increased more than 20% comparing to conventional UF. Ovine products showed higher protein levels （62-84% dry basis） what makes their manufacture attractive. Protein profiles varied with the whey origin and with the concentration process. Using batch DFvrm was possible to obtain richer protein products free of low MW compounds.

Celiac disease and skin: Psoriasis association

We read with great interest the recent review by Rodrigo on the celiac disease （CD）. The author analyzed all aspects of CD. Contrary to common belief, this disorder is a systemic disease, rather than a pure digestive alteration. In fact, CD is associated with several diseases： skin manifestations, endocrine disorders, iron-deficiency anemia, osteoporosis, hypertransaminasemia, neurologic disorders and cancer. In the description of skin manifestations, the author reported the association between CD and dermatitis herpetiformis （DH）. This condition affects about 15%-25% of patients with CD. Antibodies for CD have been detected in patients with DH. As CD and DH may be vastly different, they both share a unique intestinal sensitivity to gluten. The rash of DH is thought to be an external marker of the underlying intestinal sensitivity that is likely to be the result of molecular mimicry between the auto-antigen tissue transglutaminase resident in the gut and the skin derived epidermal transglutaminase[3]. However, our study group has reported all CD-associated skin manifestations described in the literature, and in particular psoriasis.

Fatty Acids and Triglycerides Composition in Uruguayan Cow, Sheep and Goat Cheeses

Adverse health consequences are associated with consumption of so-called trans fatty acids （FAs） generated during chemical hydrogenation processes. Naturally-occurring trans fats, including trans vaccenic acid （TVA） derived from a conjugated isomer of linoleic acid （18：2 cis-9, trans-11）, （CLA） in contrast, are recognized as having potentially beneficial effects on human health. These natural trans FAs are found in fat from dairy products and meat of ruminants, and have distinct effects compared with trans FAs industrially produced. The lipid composition of cheeses depends on the milk used. In this work was determined CLA and TVA content in milk fat of sheep and cow to be 1.4% and 3.2%, respectively, and from goat, 0.7% and 1.8%. Also, differences in CLA and TVA content were found in fat extracted from samples of the same cheese type made from cow＇s milk from the same company but with different production dates during a year. The levels of CLA and TVA found in Uruguayan cheeses were generally higher than levels reported in previous studies with comparable cheeses produced in other countries. Milk fat is well-known to confer specific properties to foods. This in turn will affect the rheology and sensory attributes as food. For this reason, it is of interest to determine the triglyceride （TAG） composition in milk-derived foods. Results show that there are characteristic differences in the TAG composition of the different cheeses. We found minimal differences between cheeses from goat and sheep but found distinct characteristics for TAGs from cow milk-derived cheese.

Solution of an Inverse Eigenvalue Problem by Newton＇s Method on a Fibre Bundle with Structure Group SO（n）

We present a differential geometric perspective of the IEP for symmetric matrices in the framework of a fibre bundle with structure group SO（n）. In particular, a Newton type algorithm is developed to construct a non singular symmetric matrix for given target eigenvalues using a singular symmetric matrix as the initial matrix for the iteration. Explicit computations are performed for 2 x 2 non singular symmetric matrix to illustrate the result.

Severe dystrophy in DiGeorge syndrome

We present the case history of a 3-year-old girl who was examined because of severe dystrophy.In the background,cow’s milk allergy was found,but her body weight was unchanged after eliminating milk from her diet.Other types of malabsorption were excluded.Based on nasal regurgitation and facial dysmorphisms,the possibility of DiGeorge syndrome was suspected and was confirmed by fluorescence in situ hybridization.The authors suggest a new feature associated with DiGeorge syndrome.

Milk Production Practices on Family Farms Located North of the State of Espirito Santo, Brazil

In Brazil, milk production is a key sector for income generation, tax collection and much of the milk produced comes from family farms. The present study was aimed to assess the conditions of milk production on 30 farms in the city of S~ Mateus, to the North of the state of Espirito Santo, Brazil. Data were obtained through questionnaires sent to producers according to the legislation of the Brazilian Ministry of Agriculture. The questions included type of milking, hygiene practices and health status of milkers, mastitis control and source and others. Milking was manually done in all farms, and only 40,0% of milkers washed their hands and forearms before milking, and 13.0% used antiseptic solutions after washing. None of the farms performed the pre-dipping and post-dipping procedures. Control of mastitis was not performed in 94.0% of the farms and none of them performed the CMT test. A high degree of non-conformity was observed in the family farms. These data demonstrate the need for training farmers in good agricultural practices. This would contribute to achieving better quality milk, and ensure the sustainability of the segment in the Northern region of the state of Espirito Santo, Brazil.

Turbulent Characteristic of Liquid Around a Chain of Bubbles in Non-Newtonian Fluid

The turbulence behavior of gas-liquid two-phase flow plays an important role in heat transfer and mass transfer in many chemical processes. In this work, a 2D particle image velocimetry (PIV) was used to investigate the turbulent characteristic of fluid induced by a chain of bubbles rising in Newtonian and non-Newtonian fluids. The instantaneous flow field, turbulent kinetic energy (TKE) and TKE dissipation rate were measured. The results demonstrated that the TKE profiles were almost symmetrical along the column center and showed higher values in the central region of the column. The TKE was enhanced with the increase of gas flow and decrease of liquid viscosity. The maximum TKE dissipation rate appeared on both sides of the bubble chain, and increased with the increase of gas flow rate or liquid viscosity. These results provide an understanding for gas-liquid mass transfer in non-Newtonian fluids.

解析几何中平面向量的坐标运算

平面向量是高中数学重要 的知识网络交汇点和数 形结合思想的重要载体．运用向量 的思想方法解决与向量有关的综 合问题,已成为高考考查数学能力 的一个方面．特别是平面向量的几 何意义、性质、数量积的坐标运算 与解析几何本身的特点(坐标化) 结合得很紧,是高考考查的重点．

浅谈报纸广告创意中的不足之处

纵观媒体广告,尤其是某些报纸的广告创意却与广告商业初衷大相径庭,有的甚至背道而驰,就报纸媒体而言,有的就存在种种弊端。