移植牛性控IVF胚胎易产生大胎综合征(LOS),严重影响胎儿个体生长发育。本试验对性控体外授精(IVF)胚胎移植效果进行了研究,并以性控IVF胚胎和人工授精(AI)胎儿骨骼肌为实验材料,选择与胎儿生长发育密切相关的基因:类胰岛素生长因子1(Ins...移植牛性控IVF胚胎易产生大胎综合征(LOS),严重影响胎儿个体生长发育。本试验对性控体外授精(IVF)胚胎移植效果进行了研究,并以性控IVF胚胎和人工授精(AI)胎儿骨骼肌为实验材料,选择与胎儿生长发育密切相关的基因:类胰岛素生长因子1(Insulin like factor 1,IGF1)、印记基因类胰岛素生长因子2(Insulin like factor 2,IGF2)、肌肉生长抑制素(Myostatin)和肌浆蛋白重链2a(Myosin heavy chain 2 a,MHC2a)为侯选基因,探讨其在性控IVF胚胎和AI胚胎中的表达差异。结果表明:母犊率为96.5%(335/347),LOS发生率为11.01%(12/103),性控IVF胚胎玻璃化冷冻移植妊娠率为34.61%(344/994),明显高于常规冷冻法移植妊娠率29.18%(218/747)(P<0.05)。性控IVF胚胎胎儿初生体质量和胸围明显高于AI胎儿(P<0.01)。在胎儿发育的不同时期,IGF1、IGF2、肌肉生长抑制素和MHC2a基因,在性控IVF胚胎和AI胚胎有明显差异,此差异可能是产生LOS的机制之一。展开更多
The volatile compounds from ash-leaf maple (Acer negundo L.) were examined by adsorption-thermodesorption and GC-MS. Thirty-two compounds, including alcohols, ketones, aldehydes, esters, terpenoids, carboxylic acids, ...The volatile compounds from ash-leaf maple (Acer negundo L.) were examined by adsorption-thermodesorption and GC-MS. Thirty-two compounds, including alcohols, ketones, aldehydes, esters, terpenoids, carboxylic acids, etc. were identified. The analysis revealed that the diurnal rhythm of release of volatile compounds from maple differed in July and in August. In July, the releasing of most volatile compounds reached the peak at 14 o'clock, when in August, the emission of volatile compounds reached the peak at 10 o'clock. Besides diurnal rhythm, there also existed other differences in the releasing of volatiles and their relative contents in July and in August. A possible explanation for this phenomenon is the maturation of leaves, since the sampling conditions were the same both in July and August. At the same time, the response of Anoplophora glabrpennis Motschulsky to volatiles was examined with field bioassay with traps. cis-3-hexen-1-ol was found to be more effective to attract A. glabrpennis than other volatiles released by ash-leaf maple tree in field trapping test. A mixture of 1-butanol, 1-pentanol and 2-pentanol was tested to be the most attractive to A. glabripennis among all tested volatiles. More field trapping tests should be conducted.展开更多
文摘移植牛性控IVF胚胎易产生大胎综合征(LOS),严重影响胎儿个体生长发育。本试验对性控体外授精(IVF)胚胎移植效果进行了研究,并以性控IVF胚胎和人工授精(AI)胎儿骨骼肌为实验材料,选择与胎儿生长发育密切相关的基因:类胰岛素生长因子1(Insulin like factor 1,IGF1)、印记基因类胰岛素生长因子2(Insulin like factor 2,IGF2)、肌肉生长抑制素(Myostatin)和肌浆蛋白重链2a(Myosin heavy chain 2 a,MHC2a)为侯选基因,探讨其在性控IVF胚胎和AI胚胎中的表达差异。结果表明:母犊率为96.5%(335/347),LOS发生率为11.01%(12/103),性控IVF胚胎玻璃化冷冻移植妊娠率为34.61%(344/994),明显高于常规冷冻法移植妊娠率29.18%(218/747)(P<0.05)。性控IVF胚胎胎儿初生体质量和胸围明显高于AI胎儿(P<0.01)。在胎儿发育的不同时期,IGF1、IGF2、肌肉生长抑制素和MHC2a基因,在性控IVF胚胎和AI胚胎有明显差异,此差异可能是产生LOS的机制之一。
文摘The volatile compounds from ash-leaf maple (Acer negundo L.) were examined by adsorption-thermodesorption and GC-MS. Thirty-two compounds, including alcohols, ketones, aldehydes, esters, terpenoids, carboxylic acids, etc. were identified. The analysis revealed that the diurnal rhythm of release of volatile compounds from maple differed in July and in August. In July, the releasing of most volatile compounds reached the peak at 14 o'clock, when in August, the emission of volatile compounds reached the peak at 10 o'clock. Besides diurnal rhythm, there also existed other differences in the releasing of volatiles and their relative contents in July and in August. A possible explanation for this phenomenon is the maturation of leaves, since the sampling conditions were the same both in July and August. At the same time, the response of Anoplophora glabrpennis Motschulsky to volatiles was examined with field bioassay with traps. cis-3-hexen-1-ol was found to be more effective to attract A. glabrpennis than other volatiles released by ash-leaf maple tree in field trapping test. A mixture of 1-butanol, 1-pentanol and 2-pentanol was tested to be the most attractive to A. glabripennis among all tested volatiles. More field trapping tests should be conducted.
