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PCR检测饲料中牛源组织成分的研究 被引量:7
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作者 钟伟军 陈金顶 《华南农业大学学报》 CAS CSCD 北大核心 2006年第3期85-88,共4页
为从动物饲料中快速检测所含牛源组织成分以有效防止疯牛病传入,根据牛线粒体基因核苷酸序列设计1对引物,应用CheleX法分离饲料中牛源组织成分的DNA.对分离的DNA进行PCR扩增、基因克隆及核苷酸序列分析,并建立饲料中牛源组织成分的PCR... 为从动物饲料中快速检测所含牛源组织成分以有效防止疯牛病传入,根据牛线粒体基因核苷酸序列设计1对引物,应用CheleX法分离饲料中牛源组织成分的DNA.对分离的DNA进行PCR扩增、基因克隆及核苷酸序列分析,并建立饲料中牛源组织成分的PCR检测方法.用建立的PCR方法对羊、猪、鸡、兔和鱼组织基因组DNA进行扩增,均无特异条带;将牛组织基因组DNA倍比稀释,分别对各个稀释度的DNA进行PCR扩增,结果表明,该方法对牛组织DNA检测的敏感度可达25.2 pgμ/L.用该研究建立的方法对含不同量牛肉粉的鱼粉进行检测,其最小检测量达0.05%(质量分数),整个过程约需3 h. 展开更多
关键词 饲料 牛源组织 PCR检测
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Anatomical and Histological Study of the Foot of Iraqi Endogenous Buffaloes (Bubalus bubalis)
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作者 Alaa A. Sawad Shymaa K. Waad 《Journal of Agricultural Science and Technology(A)》 2012年第8期1011-1017,共7页
The anatomical and histological study were conducted on 72 specimens of buffaloes foot, regardless of the age and species The foot structures were described which included first phalanx, second phalanx, third phalanx,... The anatomical and histological study were conducted on 72 specimens of buffaloes foot, regardless of the age and species The foot structures were described which included first phalanx, second phalanx, third phalanx, digital cushion, joint, ligaments and tendons, as well as the foot's sole, blood and nerve supply. The main source of blood supply is the median artery, the radial artery, dorsal metacarpal artery. On the other hand, the metacarpophalangeal joint, proximal interphalangeal joint and joint's capsules were shown by radiographic pictures. The median nerve is the main source of the nerve supply of foot, as well as ulnar and radial nerve. However, the digital tendons on the dorsal surface and palmer surface were represented by common, proper flexor digital tendons and deep superficial digital extensor tendons respectively, the histological picture of coronary region revealed that it consistd of horn tubules and intertubular horn. While the lamellae region showed that it consisted of primary epidermal lamellae, containing the epidermis and dermis regions, also, noticed that the epidermal lamellae was interfered with faced dermis lamellar. The histological structure of the hoof revealed that it consisted of keratinocytes which connected with each other by desmosal adhesion. Digital cushion contained of elastic and collagen fibers with presence of adipose cells masses greater than that at foot sole. 展开更多
关键词 Digital cushion ligament PHALANX EPIDERMIS hoof.
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