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高温处理对牛肉蛋白质化学作用力及肌原纤维蛋白结构的影响 被引量:36
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作者 康怀彬 邹良亮 +3 位作者 张慧芸 蔡超奇 王波 柯海瑞 《食品科学》 EI CAS CSCD 北大核心 2018年第23期80-86,共7页
以牛背最长肌为研究对象,对其进行高温处理(110、115、121℃分别加热3、6、9、12、15 min),通过分析蛋白质化学键、傅里叶变换红外光谱、紫外光谱、内源荧光光谱以及蛋白质片段大小等结构信息变化,探讨高温处理对牛肉蛋白质化学作用力... 以牛背最长肌为研究对象,对其进行高温处理(110、115、121℃分别加热3、6、9、12、15 min),通过分析蛋白质化学键、傅里叶变换红外光谱、紫外光谱、内源荧光光谱以及蛋白质片段大小等结构信息变化,探讨高温处理对牛肉蛋白质化学作用力及肌原纤维蛋白结构的影响。结果表明,随着处理温度的升高和加热时间的延长,牛肉蛋白中离子键和氢键含量显著下降(P<0.05),疏水相互作用和二硫键含量显著升高(P<0.05)。肌原纤维蛋白二级结构发生重排,N—H和C—N伸缩振动以及N—H弯曲振动较为明显。高温处理促使芳香族氨基酸残基暴露于分子表面,并改变了肌原纤维蛋白质疏水区域的局部结构和蛋白质的三级结构。此外,在高温处理下肌原纤维蛋白发生了明显的降解聚集,并形成了大量小分子质量的蛋白片段。可见,高温处理能够显著改变牛肉蛋白质的化学作用力及肌原纤维蛋白的结构,本研究为高温处理下牛肉蛋白质变化机制的研究提供参考。 展开更多
关键词 高温处理 牛肉蛋白质 化学作用力 肌原纤维蛋白结构
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Use of Silage Acid Devil Fish (pterygoplichthys spp.) as Protein Supplement in Finishing Beef Cattle
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作者 S. Ornelas E. Gutierrez +5 位作者 A. Juarez R. Garciduefias J. L. Espinoza M. Perea J. P. Flores G. Salas 《Journal of Agricultural Science and Technology(A)》 2011年第8期1280-1283,共4页
Animal nutrition is one of the most important limiting factors in animal production, especially in ruminants, providing proteins being the main constraint, due to the limited availability and high cost of protein sour... Animal nutrition is one of the most important limiting factors in animal production, especially in ruminants, providing proteins being the main constraint, due to the limited availability and high cost of protein sources (oilseed meals). Currently in the dam "El lnfiernillo" in Michoacan Mexico, has a large population of devil fish (Pterygoplichthys spp.), which is an economic and ecological problem, because it is not consumed by humans and causes pollution to be discarded directly into the environment. For that reason the objective of this study was to evaluate the use of silage acid devil fish (SADF) in fattening beef cattle as a protein supplement. SADF is defined as a product semi-liquid or pasty mixed with formic acid, which leads to a decrease in pH to near 4.0. Used 18 young bulls (Bos taurus × Bos indicus) for 60 days with a starting weight of 278.9 ± 51.2 kg, housed in individual pens with food and water ad libitum were randomly assigned to three treatments with different levels of inclusion SADF (0%, 12% and 18%). They were weighed to the beginning of the experiment and later every 30 days, previous fasting of 24 hours. To determine the food consumption, weigh every day the offered food and the surplus. There were no significant differences (P 〈 0.05) among treatments with different levels of inclusion of SADF with respect to daily weight gain, with values of 952 ± 324, 927 ±322 and 854 ± 307 g/day, respectively. The dry matter intake (DMI) was 8.9, 9.3 and 7.7 kg/day to 0%, 12% and 18% of SADF, respectively. In the same values for feed conversion were 9.34, 10.03 and 9.01 kg DMI/kg of weigh live, and carcass yield of 60.6%, 60.3% and 58.5%, respectively. It is concluded that fish silage acid devil is an excellent alternative in feeding beef cattle as a protein supplement. 展开更多
关键词 Fish silage acid devil fish finishing beef cattle.
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