Aim To study the reaction mechanism between gatifloxacin and bovine serumalbumin (BSA) at different pHs. Methods Fluorescence spectra and UV absorbance spectra were used.Results The binding constants were determined f...Aim To study the reaction mechanism between gatifloxacin and bovine serumalbumin (BSA) at different pHs. Methods Fluorescence spectra and UV absorbance spectra were used.Results The binding constants were determined from a double reciprocal Lineweaver-Burk curves atdifferent pHs. The binding distance r under normal physiological condition was obtained according toFoster theory of non-radiative energy transfer. The binding force between gatifloxacin and BSA wasinferred by thermody-namical coordination. Conclusion The interaction between gatifloxacin and BSAseems to be strong and the main binding force is electrostatic force.展开更多
The aim of this study was to develop a synthetic medium for the in vitro culture of bovine embryos, using various growth factors and cytokines （GF-CYK）： insulin-like growth factorl （IGF-Ⅰ）, insulin-like growth f...The aim of this study was to develop a synthetic medium for the in vitro culture of bovine embryos, using various growth factors and cytokines （GF-CYK）： insulin-like growth factorl （IGF-Ⅰ）, insulin-like growth factorⅡ （IGF-Ⅱ）, basic fibroblast growth factor （bFGF）, leukemia inhibitory factor （LIF）, granulocyte-macrophage colony stimulating factor （GM-CSF） and transforming growth factor beta Ⅰ （TGF-β1） ＋ hyaluronan （HA） ＋ recombinant albumin （RA）. The embryos were cultured in synthetic oviduct fluid （SOF） supplemented with： treatment 1 （T1）： bovine serum albumin （BSA） ＋ insulin, transferrin and selenium （ITS） （control）; or treatment 2 （T2）： GF-CYK ＋ HA ＋ RA. The blastocyst rates were not significantly different between TI and T2, at seven days post fertilization （dpf） （28.9% ± 2.4% and 31.8% ±2.2%）, and at 8 dpf （36.5% ±2.4% and 39.1% ±1.9%）, respectively （P 〉 0.05）. The total cell number （TCN） was significantly higher with T2 than that with T1 at 7 dpf（164.9 ±5.3 and 149.7 ±4.0） and 8 dpf （182.7 ±6.4 and 165.0 ±5.5） （P 〈 0.05）. The blastocyst diameter obtained with T2 was significantly greater （P 〈 0.05） than with T1 at 7 dpf （173.3 μm ±4.9 μm and 157.2μm ±4.1 μm, respectively）, however, no significant differences were observed at 8 dpf （190.3 μm 5.2 μm and 179.7 μm ± 5.3 μm, respectively）. In conclusion, the synthetic medium （T2） shows a comparable development rate to the control medium and improves the blastocyst diameter and the TCN.展开更多
In this work, the biocompatibility of a biomimetic, fully biodegradable ionomer phosphorylcholine (PC)-functionalized poly(butylene succinate) (PBS-PC) was investigated by means of hemolysis, platelet adhesion, protei...In this work, the biocompatibility of a biomimetic, fully biodegradable ionomer phosphorylcholine (PC)-functionalized poly(butylene succinate) (PBS-PC) was investigated by means of hemolysis, platelet adhesion, protein adsorption and cytotox- icity experiments. The reference materials were poly(butylene succinate) (PBS) and chloroethylphosphoryl functionalized poly(butylene succinate) (PBS-Cl). The hemolysis rates (HR) of the leaching solutions of PBS, PBS-Cl and PBS-PC were all lower than the safe value, and the rate of PBS-PC was reduced to 1.07%. Scanning electron microscopy (SEM) measurements showed that platelet adhesion and aggregation were significant on both PBS and PBS-Cl surface. In contrast, very few platelets were observed on PBS-PC surface. Bicinchoninic acid (BCA) measurements revealed that the adsorption amounts of bovine serum albumin (BSA) and bovine plasma fibrinogen (BPF) on PBS-PC surface were 52% and 72% reduction respectively compared with those on PBS surface. Moreover, non-cytotoxicity of both PBS-PC particles and its leaching solution was sug- gested by MTT assay using mouse L929 fibroblast cells. All the results demonstrated that the biocompatibility of PBS could be greatly improved by PC end-capping strategy. This PC functionalized polyester may have potential applications in biological environments as a novel carrier for controlled drug release and scaffold for tissue engineering.展开更多
文摘Aim To study the reaction mechanism between gatifloxacin and bovine serumalbumin (BSA) at different pHs. Methods Fluorescence spectra and UV absorbance spectra were used.Results The binding constants were determined from a double reciprocal Lineweaver-Burk curves atdifferent pHs. The binding distance r under normal physiological condition was obtained according toFoster theory of non-radiative energy transfer. The binding force between gatifloxacin and BSA wasinferred by thermody-namical coordination. Conclusion The interaction between gatifloxacin and BSAseems to be strong and the main binding force is electrostatic force.
文摘The aim of this study was to develop a synthetic medium for the in vitro culture of bovine embryos, using various growth factors and cytokines （GF-CYK）： insulin-like growth factorl （IGF-Ⅰ）, insulin-like growth factorⅡ （IGF-Ⅱ）, basic fibroblast growth factor （bFGF）, leukemia inhibitory factor （LIF）, granulocyte-macrophage colony stimulating factor （GM-CSF） and transforming growth factor beta Ⅰ （TGF-β1） ＋ hyaluronan （HA） ＋ recombinant albumin （RA）. The embryos were cultured in synthetic oviduct fluid （SOF） supplemented with： treatment 1 （T1）： bovine serum albumin （BSA） ＋ insulin, transferrin and selenium （ITS） （control）; or treatment 2 （T2）： GF-CYK ＋ HA ＋ RA. The blastocyst rates were not significantly different between TI and T2, at seven days post fertilization （dpf） （28.9% ± 2.4% and 31.8% ±2.2%）, and at 8 dpf （36.5% ±2.4% and 39.1% ±1.9%）, respectively （P 〉 0.05）. The total cell number （TCN） was significantly higher with T2 than that with T1 at 7 dpf（164.9 ±5.3 and 149.7 ±4.0） and 8 dpf （182.7 ±6.4 and 165.0 ±5.5） （P 〈 0.05）. The blastocyst diameter obtained with T2 was significantly greater （P 〈 0.05） than with T1 at 7 dpf （173.3 μm ±4.9 μm and 157.2μm ±4.1 μm, respectively）, however, no significant differences were observed at 8 dpf （190.3 μm 5.2 μm and 179.7 μm ± 5.3 μm, respectively）. In conclusion, the synthetic medium （T2） shows a comparable development rate to the control medium and improves the blastocyst diameter and the TCN.
基金financially supported by the National Natural Science Foundation of China (21004048, 20974087)the Natural Science Foundation of Shaanxi Educational Committe (2010JK886)the Science Foundation of Northwest University (NF0912)
文摘In this work, the biocompatibility of a biomimetic, fully biodegradable ionomer phosphorylcholine (PC)-functionalized poly(butylene succinate) (PBS-PC) was investigated by means of hemolysis, platelet adhesion, protein adsorption and cytotox- icity experiments. The reference materials were poly(butylene succinate) (PBS) and chloroethylphosphoryl functionalized poly(butylene succinate) (PBS-Cl). The hemolysis rates (HR) of the leaching solutions of PBS, PBS-Cl and PBS-PC were all lower than the safe value, and the rate of PBS-PC was reduced to 1.07%. Scanning electron microscopy (SEM) measurements showed that platelet adhesion and aggregation were significant on both PBS and PBS-Cl surface. In contrast, very few platelets were observed on PBS-PC surface. Bicinchoninic acid (BCA) measurements revealed that the adsorption amounts of bovine serum albumin (BSA) and bovine plasma fibrinogen (BPF) on PBS-PC surface were 52% and 72% reduction respectively compared with those on PBS surface. Moreover, non-cytotoxicity of both PBS-PC particles and its leaching solution was sug- gested by MTT assay using mouse L929 fibroblast cells. All the results demonstrated that the biocompatibility of PBS could be greatly improved by PC end-capping strategy. This PC functionalized polyester may have potential applications in biological environments as a novel carrier for controlled drug release and scaffold for tissue engineering.
