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基于体细胞不亲和性及ERIC-PCR研究虫草无性分离物的亲缘关系 被引量:1
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作者 唐传红 唐庆九 +2 位作者 杨焱 刘艳芳 张劲松 《食用菌》 2013年第4期20-22,36,共4页
通过对22个从虫草菌体分离到的无性菌株进行体细胞不亲和性试验,发现几乎所有菌株之间都存在程度不同的不亲和性。利用ERIC-PCR方法进行分析,发现这些供试菌株在相似性系数为0.62的水平上分成两个类群,即①蛹虫草的无性分离物(Cordyceps... 通过对22个从虫草菌体分离到的无性菌株进行体细胞不亲和性试验,发现几乎所有菌株之间都存在程度不同的不亲和性。利用ERIC-PCR方法进行分析,发现这些供试菌株在相似性系数为0.62的水平上分成两个类群,即①蛹虫草的无性分离物(Cordyceps militaris)及冬虫夏草的分离物(Elaphocordyceps subsessilis)、②蝉花的无性分离物(Lecanicillium fungicola);在相似性为0.64的水平上被分成3组,即①C.militaris、②E.subsessilis、③L.fungicola;当相似性水平高于0.92时,19个C.militaris菌株被各自分开。ERIC-PCR分析结果与体细胞不亲和性试验的结果具有较高的一致性。利用上述两种方法分析的结果都显示这些无性分离物具有丰富的遗传多样性。 展开更多
关键词 体细胞不亲和性 ERIC—PCR 无性分离 物亲缘关系
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The New Discoveries of Specific Characteristics of Yulania Spach 被引量:2
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作者 赵东欣 赵东武 孙军 《Agricultural Science & Technology》 CAS 2008年第1期54-59,共6页
The research history and new discoveries of the specific characteristics of Yulania Spach were reported, as to the specific characteristics, such as the terminal , axillary and fascicled similar flower buds,sometimes ... The research history and new discoveries of the specific characteristics of Yulania Spach were reported, as to the specific characteristics, such as the terminal , axillary and fascicled similar flower buds,sometimes polycymes; 2-4 flower types of a plant; two spathe-like stipoles of single flower; drape or overlap of tepals, deep split or shallow split on the edge of tepals. The discoveries could provide valuable information for the further studies on the taxonomic system, anthotaxical evolution, phylogenetic relationship and breeding. 展开更多
关键词 Yulania Spach Specific characteristics New discoveries
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Six new chrysophycean stomatocysts ornamented with reticulum from the Great Xing'an Mountains, China
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作者 庞婉婷 王幼芳 王全喜 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2012年第3期410-412,共3页
Six new chrysophycean stomatocysts ornamented with reticulum were illustrated based on SEM observation. They were described following the guidelines of the International Statospore Working Group (ISWG). All samples we... Six new chrysophycean stomatocysts ornamented with reticulum were illustrated based on SEM observation. They were described following the guidelines of the International Statospore Working Group (ISWG). All samples were collected from the Great Xing'an Mountains, China. Their taxonomic characteristics and habitats were described to provide new information on the biodiversity of chrysophycean stomatocysts. As is common with many morphotypes, their biological affinities remain unknown. 展开更多
关键词 chrysophycean stomatocyst China the Great Xing'an Mountains
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The BPP program for species tree estimation and species delimitation 被引量:17
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作者 Ziheng YANG 《Current Zoology》 SCIE CAS CSCD 2015年第5期854-865,共12页
This paper provides an overview and a tutorial of the BPP program, which is a Bayesian MCMC program for analyzing multi-locus genomic sequence data under the multispecies coalescent model. An example dataset of five n... This paper provides an overview and a tutorial of the BPP program, which is a Bayesian MCMC program for analyzing multi-locus genomic sequence data under the multispecies coalescent model. An example dataset of five nuclear loci from the East Asian brown frogs is used to illustrate four different analyses, including estimation of species divergence times and population size parameters under the multispecies coalescent model on a fixed species phylogeny (A00), species tree estimation when the assignment and species delimitation are fixed (A01), species delimitation using a fixed guide tree (A10), and joint species delimitation and species-tree estimation or unguided species delimitation (A11). For the joint analysis (A11), two new priors are introduced, which assign uniform probabilities for the different numbers of delimited species, which may be useful when assignment, species delimitation, and species phylogeny are all inferred in one joint analysis. The paper ends with a discussion of the assumptions, the strengths and weaknesses of the BPP analysis [Current Zoology 61 (5): 854-865, 2015]. 展开更多
关键词 BPP MCMC Multispecies coalescent Species delimitation Species tree
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Do females use their sexual status to gain resource access? Investigating food-for-sex in wolves and dogs 被引量:1
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作者 Rachel DALE Sarah MARSHALL-PESCINI Friederike RANGE 《Current Zoology》 SCIE CAS CSCD 2017年第3期323-330,共8页
While food sharing among related individuals can be explained by kin selection, food sharing be- tween unrelated individuals has been more of an evolutionary puzzle. The food-for-sex hypothesis provides an explanation... While food sharing among related individuals can be explained by kin selection, food sharing be- tween unrelated individuals has been more of an evolutionary puzzle. The food-for-sex hypothesis provides an explanation for the occurrence of food sharing among nonkin. However, little is known about the socio-ecological factors that can promote such a commodity exchange. A species mating system is a factor potentially influencing food-for-sex patterns of behavior. Here, we compared wolves, which form pair-bonds, with dogs, which are typically promiscuous in freeranging contexts, to investigate the effect of reproductive stages on the behavior around a food source in 2 different contexts. Furthermore, we considered the roles of both the males and the fe- males in the potential food-for-sex exchange. Results indicate that in both species and for both sexes the breeding period promotes decreased aggression. Additionally, females were more per- sistent in their attempts to access the food and were able to monopolize the resource more when in heat as compared to outside the breeding period. Finally, in dogs, but not wolves, females spent more time in proximity to the male's bone and had a shorter latency to start eating it when in heat. Overall, this study demonstrates that the food-for-sex hypothesis plays a part in intersexual food sharing in canids, and highlights the role of females in the interaction. These effects were especially the case in dogs, suggesting a potential effect of mating system on food-for-sex responses 展开更多
关键词 CANID food-for-sex food sharing tolerance.
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Researches on Sequence of Plant Cystatin:Phytocystatin 被引量:1
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作者 QINQingfeng HEWei +1 位作者 LIANGJun ZHANGXingyao 《Chinese Forestry Science and Technology》 2005年第2期87-94,共8页
Plant cystatins or phytocystatins are cysteine proteinase inhibitors exist widely in different plant species. Because they can kill insects by inhibiting the digestive function of the cysteine proteinase in gut, they ... Plant cystatins or phytocystatins are cysteine proteinase inhibitors exist widely in different plant species. Because they can kill insects by inhibiting the digestive function of the cysteine proteinase in gut, they are believed to play an important role in plant’s defense against pests. Phytocystatins contain the conserved QXVXG motif and show some features on their sequence different to animal cystatins. After sequencing the protein directly and the cDNA clone, a large number of plant cystatins have been characterized. A multialignment with BLAST software and a detail analysis of 38 phytocystatins show that phytocystatins possess a specific conserved amino acid sequence [LRVI]-[AGT]-[RQKE]-[FY]- [AS]-[VI]-X-[EGHDQV]-[HYFQ]-N different to the conserved sequence demonstrated by Margis in 1998. This conserved sequence can be enough to detect with exclusivity phytocystatin sequences on protein data banks. A classification of these phytocystatins is performed and they can be divided into 3 groups according to their features on amino acid sequence, and the group-I can be still divided into 3 subgroups based on the feature of their amino acid and genomic sequence. By the CLUSTALX software, the most conserved nucleotide sequences of phytocystatins were found, which could be used to design the degenerate premiers to search new phytocystatins with PCR reaction. 展开更多
关键词 multialignment conserved sequence CLASSIFICATION
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