DNA methylation is an important epigenetic regulatory mechanism that influences genomic stability, gene activation, X-chromosome inactivation and other factors. A change in DNA methylation is usually associated with a...DNA methylation is an important epigenetic regulatory mechanism that influences genomic stability, gene activation, X-chromosome inactivation and other factors. A change in DNA methylation is usually associated with aging and cellular senescence. DNA methyltransferase 1(DNMT1) is the most abundant DNA methyltransferase, and it plays an important role in maintaining the established methylation pattern during DNA replication in vertebrates. Although the effect of aging on DNA methylation has been well studied in vertebrates, little research has been conducted in invertebrates, especially in marine bivalves. In this study, we examined global DNA methylation levels in four groups of adult Zhikong scallop Chlamys farreri at different ages. The results showed that both the age and tissue type had a strong effect on the DNA methylation. In addition, a significant decrease in DNA methylation with aging(1–4 years) can be detected in mantle, kidney and hepatopancreas. We further measured the change in DNMT1 transcript abundance using quantitative reverse transcription PCR(q RT-PCR), which revealed that DNMT1 transcription significantly decreased with aging in mantle and hepatopancreas and strongly correlated with DNA methylation(R = 0.72). Our data provided greater insight into the aging-related decline of DNA methylation, which could aid in gaining a better understanding of the relationship between DNA methylation and the aging process in bivalve mollusks.展开更多
The study developed a triple-exposure color particle image velocimetry(TE-CPIV) technique associated with pressure reconstruction, and validated its feasibility. A light source with the three primary colors of red, gr...The study developed a triple-exposure color particle image velocimetry(TE-CPIV) technique associated with pressure reconstruction, and validated its feasibility. A light source with the three primary colors of red, green, and blue(R, G, and B) is produced in a time sequence by a liquid crystal display(LCD) projector. Particle images at three different instants under the color illuminations are captured in one snapshot using a color digital single-lens reflex(SLR) camera with a complementary metal-oxide semiconductor(CMOS) sensor. A contamination correction algorithm based on a specific calibration is performed on the different color layers(R layer, G layer, and B layer) of the raw color image to reduce the contaminated intensity of each color illumination on the other two color layers. The corrected intensity generates three new color layers, from which a standard cross-correlation process in the classical PIV method is used to obtain two velocity fields. Eventually, an instantaneous pressure field is reconstructed from the two velocity fields. The feasibility of TE-CPIV was tested by two experiments with a solid body rotation flow and a cylinder wake flow. The results show acceptable accuracy and robustness of the new technique. The idea of the TE-CPIV is believed to provide a simple and effective way of estimating a pressure field with low cost and high convenience.展开更多
Simultaneous multicolor photometry of fast-moving objects is discussed in this paper. In conventional astronomical photometry, the accuracy of flux and color indices of fast-moving objects is affected by the variation...Simultaneous multicolor photometry of fast-moving objects is discussed in this paper. In conventional astronomical photometry, the accuracy of flux and color indices of fast-moving objects is affected by the variations of the targets and weather conditions in space and time domains.We optimize related techniques and methods of observation and data reduction, including image cal- ibration, background fitting, targets detection and location, isophotal photometry, and flux calibration by using background stars from different fields. We consider that simultaneous multicolor data acquisition and differential flux calibration are critical for improving photometric accuracy of fast-moving objects. Our results show the photometric accuracy is better than 5% based on the observations carried out by a 1-meter telescope under ordinary, non-photometric conditions.展开更多
基金supported by the National Natural Science Foundation of China(31130054)the National High Technology Research and Development Program of China(2012AA10A401)Doctoral Fund of Ministry of Education of China(20120132130002)
文摘DNA methylation is an important epigenetic regulatory mechanism that influences genomic stability, gene activation, X-chromosome inactivation and other factors. A change in DNA methylation is usually associated with aging and cellular senescence. DNA methyltransferase 1(DNMT1) is the most abundant DNA methyltransferase, and it plays an important role in maintaining the established methylation pattern during DNA replication in vertebrates. Although the effect of aging on DNA methylation has been well studied in vertebrates, little research has been conducted in invertebrates, especially in marine bivalves. In this study, we examined global DNA methylation levels in four groups of adult Zhikong scallop Chlamys farreri at different ages. The results showed that both the age and tissue type had a strong effect on the DNA methylation. In addition, a significant decrease in DNA methylation with aging(1–4 years) can be detected in mantle, kidney and hepatopancreas. We further measured the change in DNMT1 transcript abundance using quantitative reverse transcription PCR(q RT-PCR), which revealed that DNMT1 transcription significantly decreased with aging in mantle and hepatopancreas and strongly correlated with DNA methylation(R = 0.72). Our data provided greater insight into the aging-related decline of DNA methylation, which could aid in gaining a better understanding of the relationship between DNA methylation and the aging process in bivalve mollusks.
基金supported by the National Natural Science Foundation of China(Grant Nos.11472030,11327202&11490552)the Fundamental Research Funds for the Central Universities(Grant No.YWF-16-JCTD-A-05)
文摘The study developed a triple-exposure color particle image velocimetry(TE-CPIV) technique associated with pressure reconstruction, and validated its feasibility. A light source with the three primary colors of red, green, and blue(R, G, and B) is produced in a time sequence by a liquid crystal display(LCD) projector. Particle images at three different instants under the color illuminations are captured in one snapshot using a color digital single-lens reflex(SLR) camera with a complementary metal-oxide semiconductor(CMOS) sensor. A contamination correction algorithm based on a specific calibration is performed on the different color layers(R layer, G layer, and B layer) of the raw color image to reduce the contaminated intensity of each color illumination on the other two color layers. The corrected intensity generates three new color layers, from which a standard cross-correlation process in the classical PIV method is used to obtain two velocity fields. Eventually, an instantaneous pressure field is reconstructed from the two velocity fields. The feasibility of TE-CPIV was tested by two experiments with a solid body rotation flow and a cylinder wake flow. The results show acceptable accuracy and robustness of the new technique. The idea of the TE-CPIV is believed to provide a simple and effective way of estimating a pressure field with low cost and high convenience.
文摘Simultaneous multicolor photometry of fast-moving objects is discussed in this paper. In conventional astronomical photometry, the accuracy of flux and color indices of fast-moving objects is affected by the variations of the targets and weather conditions in space and time domains.We optimize related techniques and methods of observation and data reduction, including image cal- ibration, background fitting, targets detection and location, isophotal photometry, and flux calibration by using background stars from different fields. We consider that simultaneous multicolor data acquisition and differential flux calibration are critical for improving photometric accuracy of fast-moving objects. Our results show the photometric accuracy is better than 5% based on the observations carried out by a 1-meter telescope under ordinary, non-photometric conditions.
