雌激素诱导白假丝酵母菌阴道炎小鼠模型中环氧化酶-2的表达

目的:探讨雌激素对白假丝酵母菌性阴道炎模型小鼠阴道组织中环氧化酶-2(COX-2)表达的影响及其在发病机制中的意义。方法:80只小鼠随机分为雌激素化未感染白假丝酵母菌组(E组)、未雌激素化感染白假丝酵母菌组(I组)、雌激素化感染白假丝酵母菌组(EI组)和空白对照组(C组)。制作白念珠菌性阴道炎小鼠模型,每组分别于接种后第2天、第4天、第7天和第14天取小鼠阴道组织,用免疫组化方法检测组织中COX-2的表达。结果:除C组外,各组小鼠阴道组织内第2天出现COX-2的阳性表达,持续升高至第7天达到高峰,第14天时开始下降;E组在第4、第7天COX-2免疫组化检测OD值分别为0.157±0.017和0.161±0.014,与相应C组(0.101±0.014和0.106±0.014)比较均具有显著性统计学差异(P均<0.05)。EI和I组第7天的OD值分别达到0.275±0.059和0.234±0.063,与对照组相比明显上调(P均<0.01),且EI组显著高于I组(P<0.05)。结论:在白假丝酵母菌性阴道炎小鼠模型中,雌激素能通过诱导阴道组织中COX-2的表达在其发病机制中起重要作用。

A Prognostic Model Based on Colony Stimulating Factors-related Genes in Triple-negative Breast Cancer

Objective Triple-negative breast cancer(TNBC)is the breast cancer subtype with the worst prognosis,and lacks effective therapeutic targets.Colony stimulating factors(CSFs)are cytokines that can regulate the production of blood cells and stimulate the growth and development of immune cells,playing an important role in the malignant progression of TNBC.This article aims to construct a novel prognostic model based on the expression of colony stimulating factors-related genes(CRGs),and analyze the sensitivity of TNBC patients to immunotherapy and drug therapy.Methods We downloaded CRGs from public databases and screened for differentially expressed CRGs between normal and TNBC tissues in the TCGA-BRCA database.Through LASSO Cox regression analysis,we constructed a prognostic model and stratified TNBC patients into high-risk and low-risk groups based on the colony stimulating factors-related genes risk score(CRRS).We further analyzed the correlation between CRRS and patient prognosis,clinical features,tumor microenvironment(TME)in both high-risk and low-risk groups,and evaluated the relationship between CRRS and sensitivity to immunotherapy and drug therapy.Results We identified 842 differentially expressed CRGs in breast cancer tissues of TNBC patients and selected 13 CRGs for constructing the prognostic model.Kaplan-Meier survival curves,time-dependent receiver operating characteristic curves,and other analyses confirmed that TNBC patients with high CRRS had shorter overall survival,and the predictive ability of CRRS prognostic model was further validated using the GEO dataset.Nomogram combining clinical features confirmed that CRRS was an independent factor for the prognosis of TNBC patients.Moreover,patients in the high-risk group had lower levels of immune infiltration in the TME and were sensitive to chemotherapeutic drugs such as 5-fluorouracil,ipatasertib,and paclitaxel.Conclusion We have developed a CRRS-based prognostic model composed of 13 differentially expressed CRGs,which may serve as a useful tool for predicting the prognosis of TNBC patients and guiding clinical treatment.Moreover,the key genes within this model may represent potential molecular targets for future therapies of TNBC.

A New Model on Origin and Evolution of Biology

Modified classical Boltzmann entropy as generalized entropy, then proposed Maximum Generalized Entropy Principle fusing physics and biology, and established a new model for biological origin and evolutions based on this principle, finally took protein evolution for an example to analyze. The model provided some reference for biological complexity research.

The Characteristic Patterns of Macrofaunal Fouling Assemblages in Nearshore Waters of the South China Sea

The spatio-temporal patterns of macrofaunal fouling assemblages were quantitatively investigated in the nearshore waters of the South China Sea.The work was undertaken by deploying seasonal panels at two sites(H-site,L-site) for one year,and the fouling communities on the panels were examined and analyzed.The results indicated that species composition of assemblages was obviously different between the two sites.At both sites the assemblages were characteristic with solitary dominant species throughout the year,with Amphibalanus reticulates dominating at H-site and Hydroides elegans at L-site.Shannon index and biomass of the assemblages varied with depth and season at both sites.At H-site the total biomass in summer and autumn were significantly higher than those in spring and winter,while at L-site the assemblage biomass also differed significantly among the four seasons,and the greatest biomass occurred at the depth of 2.0 m in winter.The abundance of all seasonal samples in non-metric multidimensional scaling was clustered as one group at L-site and three groups at H-site.The environmental factors were more likely to be related to the variation of fouling assemblages.Furthermore,it also suggests that in tropical seas the integrated adaptability would qualify a species for dominating a fouling assemblage despite its short life cycle,rather than the usually assumed only species with long life span.This study reveals the complexity and characteristic dynamics of macrofaunal fouling assemblages in the tropical habitats,and the results would provide valuable knowledge for biodiversity and antifouling research.

Web-Based Interactive Simulation of Weft Knitted Fabric

In order to realize parametric simulation of three-dimensional(3D)fabric structure based on web,a 3D model describing a plain knitted fabric,in which the cross-section of the yarn is circular shape and the path of the yarn is cubic B-spline curve,is proposed in this paper.With this model,the 3D simulation of the loop and of the basic structures of the knitted fabric is realized by using Virtual Reality Modeling Language(VRML).The virtual simulation scene is suitable for network transmission with freely available VRML browsers and can be translated,scaled and rotated quite arbitrarily.By using Java and External Authoring Interface(EAI)technology,the web-based interactive simulation platform of weft knitted fabric is established.The user can input type of structures,basic fabric parameters,and yarn colors interactively to obtain a more realistic simulation result in real-time.A new approach is provided to imitate the knitted fabric 3D appearance on network.

Pedestrian environment prediction with different types of on-shore building distribution

The aim of this work is to evaluate how the building distribution influences the cooling effect of water bodies. Different turbulence models, including the S-A, SKE, RNG, Realizable, Low-KE and RSM model, were evaluated, and the CFD results were compared with wind tunnel experiment. The effects of the water body were detected by analyzing the water vapor distribution around it. It is found that the RNG model is the most effective model in terms of accuracy and computational economy. Next, the RNG model was used to simulate four waterfront planning cases to predict the wind, thermal and moisture environment in urban areas around urban water bodies. The results indicate that the building distribution, especially the height of the frontal building, has a larger effect on the water vapor dispersion, and indicate that the column-type distribution has a better performance than the enclosed-type distribution.

Statistically Downscaled Summer Rainfall over the Middle-Lower Reaches of the Yangtze River

The summer rainfall over the middle-lower reaches of the Yangtze River valley (YRSR) has been estimated with a multi-linear regression model using principal atmospheric modes derived from a 500 hPa geopotential height and a 700 hPa zonal vapor flux over the domain of East Asia and the West Pacific.The model was developed using data from 1958 92 and validated with an independent prediction from 1993 2008.The independent prediction was efficient in predicting the YRSR with a correlation coefficient of 0.72 and a relative root mean square error of 18%.The downscaling model was applied to two general circulation models (GCMs) of Flexible Global Ocean-Atmosphere-Land System Model (FGOALS) and Geophysical Fluid Dynamics Laboratory coupled climate model version 2.1 (GFDL-CM2.1) to project rainfall for present and future climate under B1 and A1B emission scenarios.The downscaled results pro-vided a closer representation of the observation compared to the raw models in the present climate.In addition,compared to the inconsistent prediction directly from dif-ferent GCMs,the downscaled results provided a consistent projection for this half-century,which indicated a clear increase in the YRSR.Under the B1 emission scenario,the rainfall could increase by an average of 11.9% until 2011 25 and 17.2% until 2036 50 from the current state;under the A1B emission scenario,rainfall could increase by an average of 15.5% until 2011 25 and 25.3% until 2036 50 from the current state.Moreover,the increased rate was faster in the following decade (2011 25) than the latter of this half-century (2036 50) under both emissions.

Biological Autonomy

We argue that genuine biological autonomy, or described at human level as free will, requires taking into account quantum vacuum processes in the context of biological teleology. One faces at least three basic problems of genuine biological autonomy： （1） if biological autonomy is not physical, where does it come from？ （2） Is there a room for biological causes？ And （3） how to obtain a workable model of biological teleology？ It is shown here that the solution of all these three problems is related to the quantum vacuum. We present a short review of how this basic aspect of the fundamentals of quantum theory, although it had not been addressed for nearly 100 years, actually it was suggested by Bohr, Heisenberg, and others. Realizing that the quantum mechanical measurement problem associated with the ＂collapse＂ of the wave function is related, in the Copenhagen Interpretation of quantum mechanics, to a process between self-consciousness and the external physical environment, we are extending the issue for an explanation of the different processes occurring between living organisms and their internal environment. Definitions of genuine biological autonomy, biological aim, and biological spontaneity are presented. We propose to improve the popular two-stage model of decisions with a biological model suitable to obtain a deeper look at the nature of the mind-body problem. In the newly emerging picture biological autonomy emerges as a new, fundamental and inevitable element of the scientific worldview.

Evaluation of Surface Air Temperature Change over China and the Globe during the Twentieth Century in IAP AGCM4.0

Based on time series and linear trend analysis, the authors evaluated the performance of the fourth gen- eration atmospheric general circulation model developed at the Institute of Atmospheric Physics, Chinese Academy of Sciences （IAP AGCM4.0）, in simulating surface air temperature （SAT） during the twentieth century over China and the globe. The numerical experiment is con- ducted by driving the model with the observed sea surface temperature and sea ice. It is shown that IAP AGCM4.0 can simulate the warming trend of the global SAT, with the major wanning regions in the high latitudes of the Northern Hemisphere and the mid-latitudes of the South- ern Hemisphere. While the simulated trend over the whole globe is close to the observation, the model trader- estimates the observed trend over the continents. More- over, the model simulates the spatial distribution of SAT in China, with a bias of approximately -2℃ in eastern China, but with a more serious bias in western China. Compared with the global mean, however, the correlation coefficient between the simulation and observation in China is significantly lower, indicating that there is large uncertainty in simulating regional climate change.

Key technologies of precision demolition blasting of ultra-long urban viaduct in complicated surroundings

The key technologies of precision blasting were put forward based on the characteristics of urban via- duct blasting demolition in complicated surroundings. Initial bending instability mechanics model of reinforcing steel bar frame of blasting fragmented pier and sequenced collapsed dynamic model were established for quanti- tative blasting design. Technologies of water pressure blasting were applied in multi-cell box girder fragmenta- tion. The detonating network of non-electric duplication crossover was adopted for the safety and reliability of ultra-long delay. The rationality of blasting scheme and parameters were validated by physical model test. Harm- ful effects were forecasted and controlled by integrated protective technologies. Specialization, cooperation, pre- cision, execution （SCPE） project management method was put forward for precision management. The key tech- nologies of precision demolition blasting can provide reference for similar proiects.

Research based on the Web Shopping service Quality and Customer Satisfaction and Loyalty

The online shopping has changed people＇s traditional mode of shopping. With more and more customers participate in online shopping, and the online shopping service providers need to improve the quality of service to attract customers to improve customer satisfaction. Based on SERVQUAL model, this paper explores the association between various dimensions of service quality of the web-based shopping and customer satisfaction and service loyalty, and gives an analysis of the five dimensions of quality of service in the context of online shopping, the relationship between service quality and customer satisfaction and loyalty, and thus providing the reference for online shopping service.

Identity-based ring signature scheme based on quadratic residues

Identity-based （ID-based） ring signature has drawn great concerns in recent years and many ID-based ring signature schemes have been proposed until now. Unfortunately, all of these ID-based ring signatures are constructed from bilinear pairings, a powerful but computationally expensive primitive. Hence, ID-based ring signature without pairing is of great interest in the field of cryptography. In this paper, the authors firstly propose an ID-based ring signature scheme based on quadratic residues. The proposed scheme is proved to be existentially unforgeable against adaptive chosen message-and-identity attack under the random oracle model, assuming the hardness of factoring. The proposed scheme is more efficient than those which are constructed from bilinear pairings.

An Alternative-Service Recommending Algorithm Based on Semantic Similarity

With the development of the Internet of Things(Io T), people's lives have become increasingly convenient. It is desirable for smart home(SH) systems to integrate and leverage the enormous information available from IoT. Information can be analyzed to learn user intentions and automatically provide the appropriate services. However, existing service recommendation models typically do not consider the services that are unavailable in a user's living environment. In order to address this problem, we propose a series of semantic models for SH devices. These semantic models can be used to infer user intentions. Based on the models, we proposed a service recommendation probability model and an alternative-service recommending algorithm. The algorithm is devoted to providing appropriate alternative services when the desired service is unavailable. The algorithm has been implemented and achieves accuracy higher than traditional Hidden Markov Model(HMM). The maximum accuracy achieved is 68.3%.

Simulated Heat Sink in the Southern Ocean and Its Contribution to the Recent Hiatus Decade

A set of numerical experiments is designed and carried out to understand a heat sink in the Southern Ocean in the recent hiatus decade. By using an oceanic general circulation model, the authors focus on the contributions from two types of forcing: wind stress and thermohaline forcing. The simulated results show that the heat sink in the upper Southern Ocean comes mainly from thermohaline forcing; while in the deeper layers, wind stress forcing also plays an important role. These different contributions may be due to different physical processes for the heat budget. The combination of these two types of forcing shows a significant heat sink in the Southern Ocean in the recent hiatus decade, and this is consistent with the observations and conclusions of a similar recently published study.

Site selection of emergency material warehouse under fuzzy environment

The objective of this work was to determine the location of emergency material warehouses. For the site selection problem of emergency material warehouses, the triangular fuzzy numbers are respectively demand of the demand node, the distance between the warehouse and demand node and the cost of the warehouse, a bi-objective programming model was established with minimum total cost of the system and minimum distance between the selected emergency material warehouses and the demand node. Using the theories of fuzzy numbers, the fuzzy programming model was transformed into a determinate bi-objective mixed integer programming model and a heuristic algorithm for this model was designed. Then, the algorithm was proven to be feasible and effective through a numerical example. Analysis results show that the location of emergency material warehouse depends heavily on the values of degree a and weight wl. Accurate information of a certain emergency activity should be collected before making the decision.

Nutrient-controlled growth of Skeletonema costatum : an applied model

To model Skeletonema costatum blooms and their relationship with environmental parameters in situ, a S. costatum-specific zero-dimensional box model based on the mechanistic model Eco3M was established using physiological features. The parameters were calibrated using experimental counterparts, and simulations were compared with published laboratory findings. The resulting normalized objective function （NOF） values are less than 1.0 （and in most cases less than 0.58） and the values for the slope y （between 0.656 7-1.127 4） and R2 （between 0.806 8-0.971） are close to 1.0 for most of the sub-figures. This indicates good agreement between simulated and measured data and suggests that the model reproduces the general characteristics of S. costatum growth and use of nutrients under different N- or P-limiting conditions. The model is appropriate for further applications and can be used to test more scenarios using other nutrients.

Arctic Oscillation Responses to Black Carbon Aerosols Emitted from Major Regions

The responses of the Arctic Oscillation(AO) to global black carbon(BC) and BC emitted from major regions were compared using the atmospheric general circulation model Geophysical Fluid Dynamics Laboratory(GFDL) atmospheric general circulation model(AGCM) Atmospheric Model version 2.1(AM2.1). The results indicated that global BC could induce positive-phase AO responses, characterized by negative responses over the polar cap on 500 h Pa height fields, and zonal mean sea level pressure(SLP) decreasing while zonal wind increasing at 60°, with the opposite responses over midlatitudes. The AO indices distribution also shifted towards positive values. East Asian BC had similar impacts to that of global BC, while the responses to European BC were of opposite sign. South Asian BC and North American BC did not affect the AO significantly. Based on a simple linear assumption, we roughly estimated that the global BC emission increase could explain approximately 5% of the observed positive AO trend of +0.32 per decade during 1960 to 2000.

Synergetics as a Positivistic ＂Trick＂ for Philosophy

This paper substantiates the idea that particular science reflects only a certain angle of a complex model of the world. Guidance as to ＂adding＂ particular science data in order to achieve a holistic picture of the world is a philosophical outlook. The meaning of each relatively closed system is revealed only in the context of a more capacious system. According to the author, as the material world itself, its epistemic models as well are built according to the principle of ＂matryoshka dolls.＂ The rational and irrational are discussed in the paper as equal moments of scientific knowledge. Contrary to the importance given to randomness in synergy, the author considers if it is possible to find out the causes of all events. In order to do this, other viewpoints and structure levels should be considered. There is a hidden order in every chaos, and that is revealed only in a certain situation. The main issue is taking into account the counter action of the system with the environment.

Modelling of Baku Residential Structures on a Complex of Climatic Factors

Growth of an anthropogenic load on an environment in the second halfofXXth centuries has led to an exacerbation of many ecological problems. Today ecological risk scales cover territories of large regions. In this respect the territory of Azerbaijan is not exception, within which Baku is noted by very adverse ecological conditions. Complex natural conditions （presence of strong winds, high air temperature and solar radiation） complicate the city ecological situation even greater. In modem conditions a construction boom relates to the factors aggravating the ecological situation of Baku. Last 10 years the city is rapidly built on by multistoried buildings which deform it, hinder visual contacts to the environment and obstruct the natural aeration of Baku amphitheater. The modem multistoried buildings erected ignoring town-planning norms do not correspond with regional climatic conditions, essentially modify the territory wind regime, hamper in surrounding buildings insolation, neglect a territory temperature-humidity conditions. As a result, it is necessary to use energy overly for their adaptation to Baku conditions and creation comfortable microclimate in them that leads to the city environmental pollution. The planning decisions and construction materials applied in these buildings are also alien to Baku climatic conditions. For qualitative transformation of Baku environment and improvement of ecological characteristics of the residential areas the author has carried out the estimation of the city territory on a complex of climatic factors （aeration, insolation and thermal-humidity regimes）. For these purposes the multidimensional statistical method is used. As a result the investigated territory of Baku is divided into 5 typological areas on climatic conditions. The brief characteristic and the general recommendations on transformation are worked out by the author for each of these typological units. Results of the research can be a basis for revealing of methods and principles of town-planning and architectural-planning organization of Baku residential areas.

Evaluation of Social Environment Impact of Highway Construction Using Gray Matter-Element Information Entropy Model

Due to the importance of the social environment impact of highway construction project, an advanced evaluation is required to incorporate situations such as uncertainty, incompatibility and less information. This paper proposes a gray matter-element evaluation model based on the information entropy. The model is developed by combining both quantitative and qualitative methods, using probability theory to convert quantitative index to qualitative index, and the weight of those indexes were determined through synthesised integral weighting method, integrating matter-element theory, grey theory, and information theory. The model is then applied to evaluate the impact of the social environmental impact of highway construction project which will provide support for decision makers. Cheng-Yu highway and Shen-Da highway were selected for model application, and good results were achieved similar to the real situation.