AIM： To evaluate the effect of mitochondrial tumor ne- crosis factor receptor-associated protein-1 （TRAP-l） on the lymph node metastasis （LNM） in Chinese colorectal cancer （CRC） patients, and develop potential ...AIM： To evaluate the effect of mitochondrial tumor ne- crosis factor receptor-associated protein-1 （TRAP-l） on the lymph node metastasis （LNM） in Chinese colorectal cancer （CRC） patients, and develop potential LNM- associated biomarkers for CRC using quantitative real- time polymerase chain reaction （RT-PCR） analysis. METHODS： Differences in mitochondrial TRAP-1 gene expression between primary CRC with LNM （LNM CRC） and without LNM （non-LNM CRC） were assessed in 96 Chinese colorectal carcinoma samples using quantita- tive RT-PCR analysis, Western blotting, and confirmed with immunohistochemical assay. The relationship between clinicopathological parameters and potential diaclnostic biomarkers was also examined.RESULTS： TRAP-1 was significantly upregulated in LNM CRC compared with non-LNM CRC, which was confirmed by RT-PCR, Western blotting and immuno- histochemical assay. The expression of TRAP-1 in two different metastatic potential human colorectal cancer cell lines, LoVo and HT29, was analyzed with Western blotting. The expression level of TRAP-1 was dramati- cally higher in LoVo than in HT29. Overexpression of TRAP-1 was significantly associated with LNM （90.2% in LNM group vs 22% in non-LNM group, P 〈 0.001）, the advanced tumor node metastasis stage （89.1% in LNM group vs 26.9% in non-LNM group, P 〈 0.001）, the increased 5-year recurrence rate （82.7% in LNM group vs 22.6% in non-LNM group, P 〈 0.001） and the decreased 5-year overall survival rate （48.4% in LNM vs 83.2% in non-LNM group, P 〈 0.001）. Univariate and multivariate analyses indicated that TRAP-1 expression was an independent prognostic factor for recurrence and survival of CRC patients （Hazard ratio of 2.445 in recurrence, P = 0.017; 2.867 in survival, P = 0.028）. CONCLUSION： Mitochondria TRAP-1 affects the lymph node metastasis in CRC, and may be a potential bio- marker for LNM and a prognostic factor in CRC. Over- expression of TRAP-1 is a predictive factor for the poor outcome of colorectal cancer patients. 2012 Baishideng. All rights reserved展开更多
Objective:To establish a scientific,objective and applicable index system for evaluating out- standing biomedical scientists for science foundation of Shanghai.Methods:According to the principal in- dices that have be...Objective:To establish a scientific,objective and applicable index system for evaluating out- standing biomedical scientists for science foundation of Shanghai.Methods:According to the principal in- dices that have been used in the developed countries for evaluating their talented personnel and the reality of our country,an index system was set up to evaluate the outstanding biomedical scientists for Shanghai science foundation.The following parameters were used to simplify the indices.,correlation coefficient, multiple correlation coefficient,partial correlation coefficient,creditability,and discriminatory power. And analytic hierarchy process was used to determine the weights of each index.Results and Conclusions: The established index system is scientific and applicable;it is helpful for cultivating and evaluating out- standing biomedical scientists.展开更多
To characterize and recognize the debris flow-related deposits,the physico-mechanical performance of four deposits from the Dongyuege(DYG),Shawa(SW),Jiangjia Gully(JJG),and Gengdi(GD)debris flows in southwest China is...To characterize and recognize the debris flow-related deposits,the physico-mechanical performance of four deposits from the Dongyuege(DYG),Shawa(SW),Jiangjia Gully(JJG),and Gengdi(GD)debris flows in southwest China is investigated through laboratory analyses and tests.The four debris-flow materials can all be remolded into coherent,homogeneous cylinders with high densification and strength–porosity of 25%-36%,mean pore-throat radius of 0.46-5.89μm,median pore-throat radius of 0.43-4.28μm,P-wave velocity of 800-1200 m/s,modulus of elasticity of 28-103 MPa,unconfined compressive strength(UCS)of 220-760 kPa,and cohesion of 65-281 kPa.Based on the comparison in slurryability and formability among debris-flow deposits,granular flow deposits,fluvial deposits,residual lateritic clay and loess,whether a sediment can be cast into competent cylinders for physico-mechanical tests can be regarded as a diagnostic evidence of old debris-flow deposits.The discrepancy in physico-mechanical properties among the four debris-flow deposits suggests that the combination of foregoing physico-mechanical parameters can characterize assembling characteristics of debris flow-related sediments including grain size distribution,mineralogy,and accidental detritus.Four deposited sediments above can be surprisingly classified as hard soil-soft rocks according to UCS,and the hard soil-soft rock behaviors can advance the further understanding of debris flows.展开更多
AIM:To investigate the effects of curcumin on the expression of peroxisome proliferator-activated receptorδ(PPARδ)and related genes in HT-29 cells. METHODS:HT-29 cells were treated with curcumin (0-80μmol/L)for 24 ...AIM:To investigate the effects of curcumin on the expression of peroxisome proliferator-activated receptorδ(PPARδ)and related genes in HT-29 cells. METHODS:HT-29 cells were treated with curcumin (0-80μmol/L)for 24 h.The effects of curcumin on the morphology of HT-29 cells were studied by Hoechst 33342 staining.The activity of caspase-3 was determined using DEVD-p NA as substrate.The levels of peroxisome PPARδ,14-3-3εand vascular endothelial growth factor(VEGF)in HT-29 cells were determined by Western blotting analysis and their mRNA expression was determined by real-time quantitative RT-PCR. RESULTS:Treatment with 10-80μmol/L curcumin induced typical features of apoptosis and activated the caspase-3 in HT-29 cells.The expression of PPARδ, 14-3-3εand VEGF was reduced and the activity of β-catenin/Tcf-4 signaling was inhibited by curcumin treatment. CONCLUSION:Curcumin can induce apoptosis of HT-29 cells and down-regulate the expression of PPARδ,14-3-3εand VEGF in HT-29.展开更多
Objective To investigate the association between plasminogen activator inhibitor(PAI)-2 expression and invasive potential in hepatocellular carcinoma(HCC) cells.Methods The HCC cell lines with high,low,and non-metasta...Objective To investigate the association between plasminogen activator inhibitor(PAI)-2 expression and invasive potential in hepatocellular carcinoma(HCC) cells.Methods The HCC cell lines with high,low,and non-metastatic potentials,namely MHCC97-H,MHCC97-L,and SMMC-7721 respectively,were cultured in vitro.Matrigel invasion assay and Western blot of PAI-2 protein expression were conducted.Results The number of invaded cells in MHCC97-L was significantly higher than that in SMMC-7721(P=0.005),whereas that in MHCC97-H was higher than in MHCC97-L(P=0.017) and SMMC-7721(P=0.001).Contrarily,PAI-2 protein expression was gradually reducing from SMMC-7721,MHCC97-L,to MHCC97-H(MHCC97-H vs.MHCC97-L,P<0.001;MHCC97-H vs.SMMC-7721,P=0.001;MHCC97-L vs.SMMC-7721,P=0.001).The Pearson's correlation analysis revealed a significant negative association between invaded cell number and PAI-2 expression(r= 0.892,P=0.001).Conclusion PAI-2 expression may be negatively associated with the invasive potential of HCC.展开更多
People's livelihood in several Himalayan regions largely depends on collection,use,and trade of medicinal plants.Traditional use is generally not a problem,but commercial gathering of selected species to meet incr...People's livelihood in several Himalayan regions largely depends on collection,use,and trade of medicinal plants.Traditional use is generally not a problem,but commercial gathering of selected species to meet increasing national and international demand can result in over-exploitation.Sustainable management of medicinal plants requires a clear understanding of the respective roles,responsibilities and viewpoints of the various stakeholders involved.Through personal interviews and group discussions,this study aimed at investigating the views of two stakeholder groups on use,trade and conservation of medicinal plants in the Rasuwa district of Nepal.Local people and district and national organizations agreed that medicinal plants are collected for a combination of commercial and personal uses.Perceptions on market availability differed significantly:100 % of the respondents from district and national organizations saw markets as easily available,against only 36 % for local people.This could explain why medicinal plants were perceived by local people to contribute less to income generation than to livelihood improvement.Different viewpoints were also expressed concerning the status of medicinal plants in the district:81 % of the respondents from district and national organizationsconsidered that medicinal plants were threatened,compared to only 28 % for local people.Despite this disparity,both stakeholder groups agreed upon potential threats to medicinal plants:over-harvesting;habitat loss due to land-use change and deforestation;and over-grazing by livestock.Several challenges were identified regarding sustainable management of medicinal plants,such as ambiguous policies;lack of resources,information and infrastructures;habitat degradation;and over-exploitation.Despite these challenges,respondents agreed that the medicinal plants sector offers huge opportunities in the Rasuwa district,given resource availability,community awareness and motivation,and the priority given to the sector by governments and other agencies.Proper collaboration,communication and coordination among stakeholders are needed to grab these opportunities.展开更多
The Pomegranate （Punica Granatum）, which belongs tothe Lythraceae family, has been used for centuries in traditional Greco-Arab and Islamic medicine of its vermifuge properties and also to treat various diseases. Th...The Pomegranate （Punica Granatum）, which belongs tothe Lythraceae family, has been used for centuries in traditional Greco-Arab and Islamic medicine of its vermifuge properties and also to treat various diseases. The aim of this research was to investigate the cytotoxicity and antioxidant activities of Moroccan Pomegranate （peel, leaves, branches, flowers and corolla）. Further, the biological activities were correlated with phytochemical contents of the plant extracts. Methanolic extract from different parts of Punica Granatum was assessed for its antiproliferative activity in two human cancer （breast and colon） cells lines （MBA-MD 231 and HT-29）, through MTT （3-（4,5-dimethyl-2- thiazolyl）-2,5-diphenyl-2H-tetrazolium bromide） bioassay using cell viability and cytotoxicity indices. DPPH （2,2-diphenyl-l-picrylhydrazyl） assay was conducted to screen the antioxidant property of the extracts together with its phenolic and flavonoids content were evaluated, as well. The methanolic extract ofPunica Granatum （peel, leaves, branches, flowers and corolla） showed the highest antiproliferative activity on MBA-MD 231 （IC50 was 133.53-233.32 μg/mL） and HT-29 （IC50 was 127.58-203.24 μg/mL） cells. Antioxidants contents are distributed as follows： peel 〉 leaf 〉 flower 〉 corolla 〉 branches. The inhibitory activities required for decreasing initial DPPH by 50% are 8.27, 9.9, 10.06, 11.67 and 13.28 μg/mL, respectively. These results are in correlation with polyphenols content from corolla, peel, leaves, flower and branches are 120.7, 115, 96.65, 90.73 and 64.67 mg GAE/g dw （mg gallic acid equivalents per g dry weight） and flavonoids are 188.8, 221.7, 180.2, 193.7 and 158.5 mg QE/g dw （mg quercetin equivalents per g dry weight）. Our results show that the peel, flowers, corolla, leaves and branches of Moroccan Pomegranate may contain a lot of bioactive compounds which are responsible for strong antioxidant and cytotoxicity activities observed here. Our finding indicates the possibility of using the extracts of this plant as source of natural antioxidant and anticancer mainly for its abundant phenolic and flavonoid contents.展开更多
Objective To observe the effect of mild moxibustion on cancer-related fatigue,serum ghrelin and adiponectin in patients undergoing chemotherapy after colorectal cancer surgery.Methods Seventy patients were divided int...Objective To observe the effect of mild moxibustion on cancer-related fatigue,serum ghrelin and adiponectin in patients undergoing chemotherapy after colorectal cancer surgery.Methods Seventy patients were divided into a control group and an observation group according to the random number table method,with 35 cases in each group.Patients in both groups were treated with conventional FOLFOX4 chemotherapy regimen.Patients in the control group also received routine treatments such as symptomatic treatment of complications,health education,nutritional support,and exercise intervention.Patients in the observation group received mild moxibustion on the basis of interventions used in the control group.The Piper fatigue scale-revision(PFS-R),Karnofsky performance status(KPS)and spleen and kidney yang deficiency syndrome were scored,and serum ghrelin and adiponectin levels were measured before and after treatment.Results After treatment,total score of PFS-R in the control group did not change significantly(P>0.05),while in the observation group it was significantly reduced(P<0.05)and lower than that in the control group(P<0.05);KPS scores in both groups were increased(both P<0.05),and it was significantly higher in the observation group than in the control group(P<0.05);total score of spleen and kidney yang deficiency syndrome in the control group did not change significantly(P>0.05),while it was significantly reduced in the observation group(P<0.05)and lower than that in the control group(P<0.05);serum ghrelin and adiponectin levels in the control group had no significant changes(both P>0.05),but the levels were significantly increased in the observation group(both P<0.05)and higher than those in the control group(both P<0.05).Conclusion Based on the routine treatments,mild moxibustion can relieve fatigue,improve quality of life,and improve the symptoms of spleen and kidney yang deficiency in patients undergoing chemotherapy after colorectal cancer surgery,which may be related to the regulation of serum ghrelin and adiponectin levels.展开更多
Objective:To observe the clinical efficacy of point application with De Huang(Rhizome Rhei Crude)powder at Shenque(CV 8)plus moxa-salt hot compress on the umbilicus for preventing gastrointestinal adverse reactions af...Objective:To observe the clinical efficacy of point application with De Huang(Rhizome Rhei Crude)powder at Shenque(CV 8)plus moxa-salt hot compress on the umbilicus for preventing gastrointestinal adverse reactions after chemotherapy for non-Hodgkin lymphoma(NHL).Methods:A total of 60 cases with NHL under chemotherapy were divided into two groups by hospitalization order,with 30 cases in each group.The control group was treated with routine nursing and the observation group was additionally given point application with Da Hueng(Rhizome Rhei Crude)powder plus moxa-salt hot compress on the umbilicus,to compare the effect in preventing gastrointestinal adverse reactions after chemotherapy between the two groups.Results:The occurrence rates of constipation,nausea,vomiting and poor appetite on the second day and fifth day after chemotherapy were obviously lower in the observation group than those in the control group,with statistically significant differences between the two groups(all P〈0.05).Conclusion;The point application with De Hueng(Rhizome Rhei Crude)powder at Shenque(CV 8)plus maxa-salt hot compress on the umbilicus can produce more significant efficacy in preventing the gastrointestinal adverse reactions after chemotherapy for NHL than routine nursing.Moreover,it is simple and easy to be used and popularized.展开更多
Catch crops that are cultivated for green manure play an important role in improving soil properties. A 3-year field experi- ment was conducted to investigate the effect of catch crop （pea, Pisum sativum L.） managem...Catch crops that are cultivated for green manure play an important role in improving soil properties. A 3-year field experi- ment was conducted to investigate the effect of catch crop （pea, Pisum sativum L.） management, i.e., incorporation of catch crop in October/November （autumn） and March （spring）, and without catch crop （control）, on soil organic carbon （SOC）, microbial biomass carbon （MBC） and the activities of carbon （C）-cycle enzymes, including cellulase （Cel）, β-glucosidase （Glu） and invertase （Inv）. Additionally, soil total nitrogen （TN） and pHKcl were investigated. The catch crop was cultivated from August to October each year during 2008-2010. Soil samples were collected from the field of spring barley （Hordeum vulgate L.） that had been grown after the catch crop. Soil samples for microbial activity determination were taken in March, May, June and August in 2009, 2010 and 2011, while SOC and TN contents as well as pHKc1 were determined in March and August. The chemical properties studied did not show significant changes as influenced by the experimental factors. The use of catch crop significantly increased the MBC content and the activities of C-cycle enzymes compared to the control. When the catch crop was incorporated in spring, a significantly higher MBC content was noted in March and May compared to autumn incorporation. Moreover, the spring incorporation of the catch crop significantly increased the Glu activity （except March）, while the activities of Cel and Inv as well as the rate of soil basal respiration were usually unaffected by the time of catch crop incorporation. Greater microbial biomass and higher enzyme activities in the catch crop-treated soil, compared to the control, indicated that the application of the catch crop as a green manure could be recommended as a promising technique to increase the biological activity of the soil. Since there was no significant effect or no consistent results were obtained related to the time of catch crop incorporation, both spring and autumn applications can be recommended as a management tool to improve the status of soil properties during the growth of a subsequent crop.展开更多
基金Supported by The Grants from Shanghai Health Bureau,No.JG1103
文摘AIM： To evaluate the effect of mitochondrial tumor ne- crosis factor receptor-associated protein-1 （TRAP-l） on the lymph node metastasis （LNM） in Chinese colorectal cancer （CRC） patients, and develop potential LNM- associated biomarkers for CRC using quantitative real- time polymerase chain reaction （RT-PCR） analysis. METHODS： Differences in mitochondrial TRAP-1 gene expression between primary CRC with LNM （LNM CRC） and without LNM （non-LNM CRC） were assessed in 96 Chinese colorectal carcinoma samples using quantita- tive RT-PCR analysis, Western blotting, and confirmed with immunohistochemical assay. The relationship between clinicopathological parameters and potential diaclnostic biomarkers was also examined.RESULTS： TRAP-1 was significantly upregulated in LNM CRC compared with non-LNM CRC, which was confirmed by RT-PCR, Western blotting and immuno- histochemical assay. The expression of TRAP-1 in two different metastatic potential human colorectal cancer cell lines, LoVo and HT29, was analyzed with Western blotting. The expression level of TRAP-1 was dramati- cally higher in LoVo than in HT29. Overexpression of TRAP-1 was significantly associated with LNM （90.2% in LNM group vs 22% in non-LNM group, P 〈 0.001）, the advanced tumor node metastasis stage （89.1% in LNM group vs 26.9% in non-LNM group, P 〈 0.001）, the increased 5-year recurrence rate （82.7% in LNM group vs 22.6% in non-LNM group, P 〈 0.001） and the decreased 5-year overall survival rate （48.4% in LNM vs 83.2% in non-LNM group, P 〈 0.001）. Univariate and multivariate analyses indicated that TRAP-1 expression was an independent prognostic factor for recurrence and survival of CRC patients （Hazard ratio of 2.445 in recurrence, P = 0.017; 2.867 in survival, P = 0.028）. CONCLUSION： Mitochondria TRAP-1 affects the lymph node metastasis in CRC, and may be a potential bio- marker for LNM and a prognostic factor in CRC. Over- expression of TRAP-1 is a predictive factor for the poor outcome of colorectal cancer patients. 2012 Baishideng. All rights reserved
文摘Objective:To establish a scientific,objective and applicable index system for evaluating out- standing biomedical scientists for science foundation of Shanghai.Methods:According to the principal in- dices that have been used in the developed countries for evaluating their talented personnel and the reality of our country,an index system was set up to evaluate the outstanding biomedical scientists for Shanghai science foundation.The following parameters were used to simplify the indices.,correlation coefficient, multiple correlation coefficient,partial correlation coefficient,creditability,and discriminatory power. And analytic hierarchy process was used to determine the weights of each index.Results and Conclusions: The established index system is scientific and applicable;it is helpful for cultivating and evaluating out- standing biomedical scientists.
基金Project(41931294)supported by the National Natural Science Foundation of ChinaProjects(U1502232,U1033601)supported by the National Natural Science Foundation of China-Yunnan Joint Fund。
文摘To characterize and recognize the debris flow-related deposits,the physico-mechanical performance of four deposits from the Dongyuege(DYG),Shawa(SW),Jiangjia Gully(JJG),and Gengdi(GD)debris flows in southwest China is investigated through laboratory analyses and tests.The four debris-flow materials can all be remolded into coherent,homogeneous cylinders with high densification and strength–porosity of 25%-36%,mean pore-throat radius of 0.46-5.89μm,median pore-throat radius of 0.43-4.28μm,P-wave velocity of 800-1200 m/s,modulus of elasticity of 28-103 MPa,unconfined compressive strength(UCS)of 220-760 kPa,and cohesion of 65-281 kPa.Based on the comparison in slurryability and formability among debris-flow deposits,granular flow deposits,fluvial deposits,residual lateritic clay and loess,whether a sediment can be cast into competent cylinders for physico-mechanical tests can be regarded as a diagnostic evidence of old debris-flow deposits.The discrepancy in physico-mechanical properties among the four debris-flow deposits suggests that the combination of foregoing physico-mechanical parameters can characterize assembling characteristics of debris flow-related sediments including grain size distribution,mineralogy,and accidental detritus.Four deposited sediments above can be surprisingly classified as hard soil-soft rocks according to UCS,and the hard soil-soft rock behaviors can advance the further understanding of debris flows.
文摘AIM:To investigate the effects of curcumin on the expression of peroxisome proliferator-activated receptorδ(PPARδ)and related genes in HT-29 cells. METHODS:HT-29 cells were treated with curcumin (0-80μmol/L)for 24 h.The effects of curcumin on the morphology of HT-29 cells were studied by Hoechst 33342 staining.The activity of caspase-3 was determined using DEVD-p NA as substrate.The levels of peroxisome PPARδ,14-3-3εand vascular endothelial growth factor(VEGF)in HT-29 cells were determined by Western blotting analysis and their mRNA expression was determined by real-time quantitative RT-PCR. RESULTS:Treatment with 10-80μmol/L curcumin induced typical features of apoptosis and activated the caspase-3 in HT-29 cells.The expression of PPARδ, 14-3-3εand VEGF was reduced and the activity of β-catenin/Tcf-4 signaling was inhibited by curcumin treatment. CONCLUSION:Curcumin can induce apoptosis of HT-29 cells and down-regulate the expression of PPARδ,14-3-3εand VEGF in HT-29.
基金Supported by the National High Technology Research and Development Program of China (863 Program,2006AA02A308)the National Key Technologies Research and Development Program of China (2008ZX10002-016)the Beijing Municipal Key Project(H030230280410)
文摘Objective To investigate the association between plasminogen activator inhibitor(PAI)-2 expression and invasive potential in hepatocellular carcinoma(HCC) cells.Methods The HCC cell lines with high,low,and non-metastatic potentials,namely MHCC97-H,MHCC97-L,and SMMC-7721 respectively,were cultured in vitro.Matrigel invasion assay and Western blot of PAI-2 protein expression were conducted.Results The number of invaded cells in MHCC97-L was significantly higher than that in SMMC-7721(P=0.005),whereas that in MHCC97-H was higher than in MHCC97-L(P=0.017) and SMMC-7721(P=0.001).Contrarily,PAI-2 protein expression was gradually reducing from SMMC-7721,MHCC97-L,to MHCC97-H(MHCC97-H vs.MHCC97-L,P<0.001;MHCC97-H vs.SMMC-7721,P=0.001;MHCC97-L vs.SMMC-7721,P=0.001).The Pearson's correlation analysis revealed a significant negative association between invaded cell number and PAI-2 expression(r= 0.892,P=0.001).Conclusion PAI-2 expression may be negatively associated with the invasive potential of HCC.
文摘People's livelihood in several Himalayan regions largely depends on collection,use,and trade of medicinal plants.Traditional use is generally not a problem,but commercial gathering of selected species to meet increasing national and international demand can result in over-exploitation.Sustainable management of medicinal plants requires a clear understanding of the respective roles,responsibilities and viewpoints of the various stakeholders involved.Through personal interviews and group discussions,this study aimed at investigating the views of two stakeholder groups on use,trade and conservation of medicinal plants in the Rasuwa district of Nepal.Local people and district and national organizations agreed that medicinal plants are collected for a combination of commercial and personal uses.Perceptions on market availability differed significantly:100 % of the respondents from district and national organizations saw markets as easily available,against only 36 % for local people.This could explain why medicinal plants were perceived by local people to contribute less to income generation than to livelihood improvement.Different viewpoints were also expressed concerning the status of medicinal plants in the district:81 % of the respondents from district and national organizationsconsidered that medicinal plants were threatened,compared to only 28 % for local people.Despite this disparity,both stakeholder groups agreed upon potential threats to medicinal plants:over-harvesting;habitat loss due to land-use change and deforestation;and over-grazing by livestock.Several challenges were identified regarding sustainable management of medicinal plants,such as ambiguous policies;lack of resources,information and infrastructures;habitat degradation;and over-exploitation.Despite these challenges,respondents agreed that the medicinal plants sector offers huge opportunities in the Rasuwa district,given resource availability,community awareness and motivation,and the priority given to the sector by governments and other agencies.Proper collaboration,communication and coordination among stakeholders are needed to grab these opportunities.
文摘The Pomegranate （Punica Granatum）, which belongs tothe Lythraceae family, has been used for centuries in traditional Greco-Arab and Islamic medicine of its vermifuge properties and also to treat various diseases. The aim of this research was to investigate the cytotoxicity and antioxidant activities of Moroccan Pomegranate （peel, leaves, branches, flowers and corolla）. Further, the biological activities were correlated with phytochemical contents of the plant extracts. Methanolic extract from different parts of Punica Granatum was assessed for its antiproliferative activity in two human cancer （breast and colon） cells lines （MBA-MD 231 and HT-29）, through MTT （3-（4,5-dimethyl-2- thiazolyl）-2,5-diphenyl-2H-tetrazolium bromide） bioassay using cell viability and cytotoxicity indices. DPPH （2,2-diphenyl-l-picrylhydrazyl） assay was conducted to screen the antioxidant property of the extracts together with its phenolic and flavonoids content were evaluated, as well. The methanolic extract ofPunica Granatum （peel, leaves, branches, flowers and corolla） showed the highest antiproliferative activity on MBA-MD 231 （IC50 was 133.53-233.32 μg/mL） and HT-29 （IC50 was 127.58-203.24 μg/mL） cells. Antioxidants contents are distributed as follows： peel 〉 leaf 〉 flower 〉 corolla 〉 branches. The inhibitory activities required for decreasing initial DPPH by 50% are 8.27, 9.9, 10.06, 11.67 and 13.28 μg/mL, respectively. These results are in correlation with polyphenols content from corolla, peel, leaves, flower and branches are 120.7, 115, 96.65, 90.73 and 64.67 mg GAE/g dw （mg gallic acid equivalents per g dry weight） and flavonoids are 188.8, 221.7, 180.2, 193.7 and 158.5 mg QE/g dw （mg quercetin equivalents per g dry weight）. Our results show that the peel, flowers, corolla, leaves and branches of Moroccan Pomegranate may contain a lot of bioactive compounds which are responsible for strong antioxidant and cytotoxicity activities observed here. Our finding indicates the possibility of using the extracts of this plant as source of natural antioxidant and anticancer mainly for its abundant phenolic and flavonoid contents.
文摘Objective To observe the effect of mild moxibustion on cancer-related fatigue,serum ghrelin and adiponectin in patients undergoing chemotherapy after colorectal cancer surgery.Methods Seventy patients were divided into a control group and an observation group according to the random number table method,with 35 cases in each group.Patients in both groups were treated with conventional FOLFOX4 chemotherapy regimen.Patients in the control group also received routine treatments such as symptomatic treatment of complications,health education,nutritional support,and exercise intervention.Patients in the observation group received mild moxibustion on the basis of interventions used in the control group.The Piper fatigue scale-revision(PFS-R),Karnofsky performance status(KPS)and spleen and kidney yang deficiency syndrome were scored,and serum ghrelin and adiponectin levels were measured before and after treatment.Results After treatment,total score of PFS-R in the control group did not change significantly(P>0.05),while in the observation group it was significantly reduced(P<0.05)and lower than that in the control group(P<0.05);KPS scores in both groups were increased(both P<0.05),and it was significantly higher in the observation group than in the control group(P<0.05);total score of spleen and kidney yang deficiency syndrome in the control group did not change significantly(P>0.05),while it was significantly reduced in the observation group(P<0.05)and lower than that in the control group(P<0.05);serum ghrelin and adiponectin levels in the control group had no significant changes(both P>0.05),but the levels were significantly increased in the observation group(both P<0.05)and higher than those in the control group(both P<0.05).Conclusion Based on the routine treatments,mild moxibustion can relieve fatigue,improve quality of life,and improve the symptoms of spleen and kidney yang deficiency in patients undergoing chemotherapy after colorectal cancer surgery,which may be related to the regulation of serum ghrelin and adiponectin levels.
文摘Objective:To observe the clinical efficacy of point application with De Huang(Rhizome Rhei Crude)powder at Shenque(CV 8)plus moxa-salt hot compress on the umbilicus for preventing gastrointestinal adverse reactions after chemotherapy for non-Hodgkin lymphoma(NHL).Methods:A total of 60 cases with NHL under chemotherapy were divided into two groups by hospitalization order,with 30 cases in each group.The control group was treated with routine nursing and the observation group was additionally given point application with Da Hueng(Rhizome Rhei Crude)powder plus moxa-salt hot compress on the umbilicus,to compare the effect in preventing gastrointestinal adverse reactions after chemotherapy between the two groups.Results:The occurrence rates of constipation,nausea,vomiting and poor appetite on the second day and fifth day after chemotherapy were obviously lower in the observation group than those in the control group,with statistically significant differences between the two groups(all P〈0.05).Conclusion;The point application with De Hueng(Rhizome Rhei Crude)powder at Shenque(CV 8)plus maxa-salt hot compress on the umbilicus can produce more significant efficacy in preventing the gastrointestinal adverse reactions after chemotherapy for NHL than routine nursing.Moreover,it is simple and easy to be used and popularized.
文摘Catch crops that are cultivated for green manure play an important role in improving soil properties. A 3-year field experi- ment was conducted to investigate the effect of catch crop （pea, Pisum sativum L.） management, i.e., incorporation of catch crop in October/November （autumn） and March （spring）, and without catch crop （control）, on soil organic carbon （SOC）, microbial biomass carbon （MBC） and the activities of carbon （C）-cycle enzymes, including cellulase （Cel）, β-glucosidase （Glu） and invertase （Inv）. Additionally, soil total nitrogen （TN） and pHKcl were investigated. The catch crop was cultivated from August to October each year during 2008-2010. Soil samples were collected from the field of spring barley （Hordeum vulgate L.） that had been grown after the catch crop. Soil samples for microbial activity determination were taken in March, May, June and August in 2009, 2010 and 2011, while SOC and TN contents as well as pHKc1 were determined in March and August. The chemical properties studied did not show significant changes as influenced by the experimental factors. The use of catch crop significantly increased the MBC content and the activities of C-cycle enzymes compared to the control. When the catch crop was incorporated in spring, a significantly higher MBC content was noted in March and May compared to autumn incorporation. Moreover, the spring incorporation of the catch crop significantly increased the Glu activity （except March）, while the activities of Cel and Inv as well as the rate of soil basal respiration were usually unaffected by the time of catch crop incorporation. Greater microbial biomass and higher enzyme activities in the catch crop-treated soil, compared to the control, indicated that the application of the catch crop as a green manure could be recommended as a promising technique to increase the biological activity of the soil. Since there was no significant effect or no consistent results were obtained related to the time of catch crop incorporation, both spring and autumn applications can be recommended as a management tool to improve the status of soil properties during the growth of a subsequent crop.
