The liver proteome can serve as a reference to better understand both disease mechanisms and possible therapeutics,since the liver is an important organ in the body that performs a large number of tasks.Here we identi...The liver proteome can serve as a reference to better understand both disease mechanisms and possible therapeutics,since the liver is an important organ in the body that performs a large number of tasks.Here we identify the organelle proteome of C57BL/6J mouse liver nuclei as a promising strategy to enrich low abundance proteins,in the sense that analysis of whole liver cells is rather complex for current techniques and may not be suitable for proteins with low abundance.Evaluation of nucleus integrity and purity was performed to demonstrate the effectiveness of the optimized isolation procedure.The extracted nuclear proteins were identified by 2-DE MS analyses,and a total of 748 proteins were identified.Bioinformatic analyses were performed to demonstrate the physicochemical properties,cellular locations and functions of the proteins.展开更多
Application of organic waste to saline alkaline soils is considered to be a good practice for soil remediation. The effects of applying different organic amendments （e.g., cattle dung, vermicompost, biofertilizer） a...Application of organic waste to saline alkaline soils is considered to be a good practice for soil remediation. The effects of applying different organic amendments （e.g., cattle dung, vermicompost, biofertilizer） and earthworm inoculations （Eisenia fetida） on saline soils and cotton growth were investigated during i year of cotton cultivation. Compared to the control （applied with inorganic NPK fertilizer）, applying organic amendments improved soil physicochemical properties. Biofertilizer application improved available nutrient content, reduced short-term soil electrical conductivity, and produced the highest cotton yield, whereas cattle dung and vermicompost applications resulted in higher soil organic matter content. Application of organic amendments significantly increased soil microbial biomass carbon during the flowering period, which sharply declined at harvest. This was especially true for the biofertilizer treatment, which also exhibited lower nematode abundance compared with the other organic materials. Earthworm inoculation following cattle dung application failed to significantly change soil physicochemical properties when compared to the treatments without earthworm inoculation. Results suggest that biofertilizer application to saline soil would improve soil nutrient status in the short-term, whereas cattle dung application would improve soil organic matter content and increase soil organism abundance to a greater extent. However, different strategies might be required for long-term saline soil remediation.展开更多
基金supported by the National Basic Research Program of China(2013CB910802,2012CB910602,2010CB912704)National High Technology Research and Development Program of China (2012AA020201,2012AA020202)+1 种基金State Key Project Specialized for Infectious Diseases of China (2012ZX10002-012)National Natural Science Foundation of China (30700990,20975024,31000379)
文摘The liver proteome can serve as a reference to better understand both disease mechanisms and possible therapeutics,since the liver is an important organ in the body that performs a large number of tasks.Here we identify the organelle proteome of C57BL/6J mouse liver nuclei as a promising strategy to enrich low abundance proteins,in the sense that analysis of whole liver cells is rather complex for current techniques and may not be suitable for proteins with low abundance.Evaluation of nucleus integrity and purity was performed to demonstrate the effectiveness of the optimized isolation procedure.The extracted nuclear proteins were identified by 2-DE MS analyses,and a total of 748 proteins were identified.Bioinformatic analyses were performed to demonstrate the physicochemical properties,cellular locations and functions of the proteins.
基金supported by the National Natural Science Foundation of China (No. 41371305)the National Key Research and Development Program of China (No. 2017YFD0202000)
文摘Application of organic waste to saline alkaline soils is considered to be a good practice for soil remediation. The effects of applying different organic amendments （e.g., cattle dung, vermicompost, biofertilizer） and earthworm inoculations （Eisenia fetida） on saline soils and cotton growth were investigated during i year of cotton cultivation. Compared to the control （applied with inorganic NPK fertilizer）, applying organic amendments improved soil physicochemical properties. Biofertilizer application improved available nutrient content, reduced short-term soil electrical conductivity, and produced the highest cotton yield, whereas cattle dung and vermicompost applications resulted in higher soil organic matter content. Application of organic amendments significantly increased soil microbial biomass carbon during the flowering period, which sharply declined at harvest. This was especially true for the biofertilizer treatment, which also exhibited lower nematode abundance compared with the other organic materials. Earthworm inoculation following cattle dung application failed to significantly change soil physicochemical properties when compared to the treatments without earthworm inoculation. Results suggest that biofertilizer application to saline soil would improve soil nutrient status in the short-term, whereas cattle dung application would improve soil organic matter content and increase soil organism abundance to a greater extent. However, different strategies might be required for long-term saline soil remediation.
