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物理应激对大蒜培养细胞SOD的诱导 被引量:3
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作者 曾庆平 郭勇 《药物生物技术》 CAS CSCD 1998年第3期153-156,共4页
采用物理应激因素处理大蒜培养细胞进行了超氧化物歧化酶 (SOD)高效诱导条件的探索。结果发现 :冷胁迫和热休克能使 SOD活力升高 ,冷胁迫和间歇热休克可以诱导更高的SOD活力。冷胁迫诱导的 SOD,在光照下为 Fe.SOD和 Mn.SOD,黑暗中则为 C... 采用物理应激因素处理大蒜培养细胞进行了超氧化物歧化酶 (SOD)高效诱导条件的探索。结果发现 :冷胁迫和热休克能使 SOD活力升高 ,冷胁迫和间歇热休克可以诱导更高的SOD活力。冷胁迫诱导的 SOD,在光照下为 Fe.SOD和 Mn.SOD,黑暗中则为 Cu/ Zn.SOD;热休克诱导的 SOD,无论光照或黑暗 ,均以 Cu/ Zn. 展开更多
关键词 物理应激 冷胁迫 热休克 SOD 诱导 大蒜
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限制饮水对大鼠脑核团内蛋白激酶ERK1/2磷酸化水平的影响 被引量:1
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作者 朱正华 王百忍 +3 位作者 段小莉 邝芳 王曦 鞠躬 《解剖学报》 CAS CSCD 北大核心 2006年第4期361-366,共6页
目的研究限制饮水应激对大鼠脑内磷酸化蛋白激酶ERK1/2(pERK1/2)水平的影响。方法58只雄性Wistar大鼠分为对照组(C,n=4)、定时给水组(TW,n=18)、空瓶刺激组(EB,n=18)、限制饮水组(WR,n=18),后3组动物同置一室。TW组、EB组和WR组再按限... 目的研究限制饮水应激对大鼠脑内磷酸化蛋白激酶ERK1/2(pERK1/2)水平的影响。方法58只雄性Wistar大鼠分为对照组(C,n=4)、定时给水组(TW,n=18)、空瓶刺激组(EB,n=18)、限制饮水组(WR,n=18),后3组动物同置一室。TW组、EB组和WR组再按限制饮水时间分为3d、7d和14d 3个亚组。各实验组在最后一次实验刺激后1 h用免疫组织化学方法观察脑内pERK1/2表达的变化,并对相关核团中pERK1/2阳性细胞的面积进行图像分析。结果与对照组相比,实验刺激后引起大鼠脑内下丘脑室旁核(PVN)、视上核(SON)、腹外侧隔区(LSV)、内侧杏仁核(MeA)、中央杏仁核(CeA)和孤束核(NTS)等核团的pERK1/2表达显著增强;其中,EB组和WR组在SON、LSV、MeA和CeA pERK1/2的表达均显著强于TW组,且EB组在LSV、MeA、CeA和NTS pERK1/2的表达增强和回落的时间要显著早于WR组。除PVN仅与刺激时间有关外,其余核团均与刺激时间和方式双重因素有关。结论限制饮水应激激活脑内PVN、SON、LSV、MeA、CeA和NTS等核团。物理性应激和心理性应激激活的中枢核团活动有一定差别。 展开更多
关键词 磷酸化蛋白激酶 心理应激 物理应激 限制饮水 免疫组织化学 大鼠
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Body awareness therapy: A new strategy for relief of symptoms in irritable bowel syndrome patients 被引量:1
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作者 EM Eriksson IE Mller +2 位作者 RH Sderberg HT Eriksson GK Kurlberg 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第23期3206-3214,共9页
AIM: To compare irritable bowel syndrome (IBS) pa- tients with apparently healthy persons and to evaluate body awareness therapy, which is a physiotherapeutic remedy focusing on normalising tensions in the body, for t... AIM: To compare irritable bowel syndrome (IBS) pa- tients with apparently healthy persons and to evaluate body awareness therapy, which is a physiotherapeutic remedy focusing on normalising tensions in the body, for the treatment of IBS with the hypothesis that altered body tension is associated with the syndrome. METHODS: Twenty-one IBS patients received body awareness therapy two hours weekly for 24 wk. At base- line as well as after 12 and 24 wk, they underwent ex- aminations including resource oriented body examination in combination with body awareness scale evaluation and f illed in gastrointestinal and psychological symptom questionnaires. Saliva cortisol was analysed. A group of 21 apparently healthy persons underwent the same ex- aminations once. RESULTS: Compared to the apparently healthy group, IBS patients scored higher at baseline for gastrointestinal and psychological symptoms. They showed more often alterations in normal body tension patterns, as well as deviating cortisol slopes in saliva. After 24 wk of body awareness therapy, their gastrointestinal and psychologi- cal symptoms were reduced overall. Somatic symptoms decreased in parallel with depressive symptoms. Whole body pain score decreased, coping ability as well as bio- chemical stress markers improved. CONCLUSION: IBS patients scored higher for gastroin- testinal and psychological symptoms, and presented with altered biochemical stress markers. Their body tension deviated compared to healthy controls. Furthermore,body awareness therapy gave relief of both somatic com- plaints, psychological symptoms and normalised body tension. These findings indicate that distorted tension constitutes an important part of the symptoms in IBS. 展开更多
关键词 Irritable bowel syndrome Pain CORTISOL PHYSIOTHERAPY Body awareness therapy PSYCHOSOMATICS Gastrointestinal tract Stress
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Effect of psychological stress on gastric motility assessed by electrical bio-impedance 被引量:6
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作者 María Raquel Huerta-Franco Miguel Vargas-Luna +2 位作者 Juana Berenice Montes-Frausto Ismael Morales-Mata Lorena Ramirez-Padilla 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第36期5027-5033,共7页
AIM:To evaluate gastric motility using electrical bioimpedance(EBI) and gastric changes as a result of stress induced by psychological tests.METHODS:A group of 57 healthy women,aged 40-60 years,was recruited,and a cli... AIM:To evaluate gastric motility using electrical bioimpedance(EBI) and gastric changes as a result of stress induced by psychological tests.METHODS:A group of 57 healthy women,aged 40-60 years,was recruited,and a clinical history and physical examination were performed.The women were free from severe anxiety,chronic or acute stress,severe depression,mental diseases and conditions that affect gastric activity.The women were evaluated under fasting conditions,and using a four-electrode configuration,the gastric signals were obtained through a BIOPAC MP-150 system.The volunteers were evaluated using the following paradigm:basal state,recording during the Stroop Test,intermediate resting period,recording during the Raven Test,and a final resting period.We analyzed the relative areas of the frequency spectrum:A1(1-2 cpm),A2(2-4 cpm),A3(4-8 cpm),and A4(8-12 cpm),as well as the median of area A2 + A3.The data were analyzed by an autoregressive method using a Butterworth filter with MatLab and Origin.Analysis of variance(ANOVA) and Friedman ANOVA(for nonparametric variables) were performed;in addition,pairs of groups were compared using the T dependent and Wilcoxon T tests.RESULTS:The results of the main values of area A2 were not significantly different comparing the five steps of the experimental paradigm.Nevertheless,there was a tendency of this A2 region to decrease during the stress tests,with recuperation at the final resting step.When an extended gastric region was considered(1-4 cpm),significant differences with the psychological stress tests were present(F = 3.85,P = 0.005).The A3 region also showed significant changes when the stress psychological tests were administered(F = 7.25,P < 0.001).These differences were influenced by the changes in the adjacent gastric region of A2.The parameter that we proposed in previous studies for the evaluation of gastric motility by electrical bio-impedance(EBI) was the median of the area under the region from 2 to 8 cpm(A2 + A3).The mean values of these frequencies(median of the A2 + A3 area) with the stress test showed significant changes(F = 5.5,P < 0.001).The results of the WilcoxonT test for the A4 area parameter,which is influenced by the breathing response,changed significantly during the Raven stress test(P < 0.05).CONCLUSION:We confirm that the gastric response to acute psychological stress can be evaluated by short-term EBI. 展开更多
关键词 Psychological stress BIO-IMPEDANCE Gas-tric motility
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Effect of Transport Time and Handling on Physiological Responses of Cattle 被引量:1
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作者 Girma Gebresenbet Isabel Wikner +2 位作者 Emmanuel Yaovi Hunnuor Bobobee Gustavo Maria Morris Villarroel 《Journal of Agricultural Science and Technology(A)》 2012年第6期800-814,共15页
The objective of this work was to determine the effect of transport time (up to 11 hours) on animal welfare. 540 animals (cows, bulls and calves), three transport times of 2, 4 to 6, and 10 to 11 hours, and two sp... The objective of this work was to determine the effect of transport time (up to 11 hours) on animal welfare. 540 animals (cows, bulls and calves), three transport times of 2, 4 to 6, and 10 to 11 hours, and two space allowances (2 m2 and 1.5 m2 per animal for cows and bulls; and 1.2 m2 and 0.8 m2 per animal for calves) were used for the experiment during transport from farms to the abattoir in Uppsala, Sweden. Measurements were made on five animals on each trip. Stress response parameters considered were cortisol, glucose, lactate, creatine kinase and heart rate. Blood samples were taken before and after transport. Heart rate sensors were mounted on the animals at least 30 minutes before loading and measurements were made continuously from farms to the abattoirs until stunning. The results of heart rate measurement indicated that loading, un-loading and forced movement in the stunning box were the most stressful events. However, the results of statistical analysis confirmed that transport time doesn't have significant effect (P 〈 0.05) on heart rate. Concentration level of cortisol increased by 10 folds during short transport. However, cortisol concentration decreased with an increase of transport times (P 〈 0.01). Glucose concentrations increased with transport time in all animal categories (P ≤ 0.01). Transport time has significant effect on concentration levels of glucose (P ≤ 0.01), creatine kinase (P 〈 0.001) and lactate (P 〈 0.01) particularly after 6 hours journey time. 展开更多
关键词 Transport time CATTLE WELFARE physiological parameters
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To understand moxibustion from the biological effect of local thermal stimulation 被引量:4
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作者 黄凯裕 梁爽 +2 位作者 陆露 Patrick John Morgan 张建斌 《World Journal of Acupuncture-Moxibustion》 CSCD 2016年第3期31-37,48,共8页
Moxibustion is one of treatment modalities in Traditional Chinese Medicine(TCM). Its working mechanism is activated through heat-induced local thermal stimulation. By reviewing and compiling clear, scientific analys... Moxibustion is one of treatment modalities in Traditional Chinese Medicine(TCM). Its working mechanism is activated through heat-induced local thermal stimulation. By reviewing and compiling clear, scientific analysis of the biological and physiological effects from local thermal heat, the authors provides a coherent and systemic source to aid us to form an understanding of the mechanism of moxibustion’s promotion of qi and blood circulation throughout the body from local thermal stimulation. This has been done by a rigorous review of previous studies of the thermal effect on the body. The following presents the local, distal and systemic biological effects from local thermal stimulation while also discussing the similarities and differences between local thermal stimulation and moxibustion. 展开更多
关键词 MOXIBUSTION RULE local thermal stimulation the biological effect
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