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甲基营养菌MP688基因组完成图构建
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作者 智静娟 熊向华 +3 位作者 何建勇 汪建华 何涛 张惟材 《生物技术通讯》 CAS 2011年第4期493-496,共4页
目的:在Solexa测序建立的甲基营养菌MP688基因组精细图的基础上,构建MP688基因组完成图。方法:根据MP688基因组序列设计引物,进行常规PCR扩增,填补Scaffold内部缺口;利用BLASTN、BLAT软件分析预测6个Scaffold定位关系;通过组合PCR、长距... 目的:在Solexa测序建立的甲基营养菌MP688基因组精细图的基础上,构建MP688基因组完成图。方法:根据MP688基因组序列设计引物,进行常规PCR扩增,填补Scaffold内部缺口;利用BLASTN、BLAT软件分析预测6个Scaffold定位关系;通过组合PCR、长距离PCR等填充Scaffold间的物理缺口。结果:常规PCR填充Scaffold内部4个缺口;BLASTN、BLAT软件分析预测了Scaffold定位关系;组合PCR填充Scaffold间的2个物理缺口,长距离PCR填充其余4个物理缺口。结论:得到了MP688的基因组完成图,为MP688的吡咯喹啉醌生物合成途径的阐明和进一步的代谢工程育种奠定了基础。 展开更多
关键词 甲基营养菌 基因组完成图 物理缺口
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A Modified Sensitive Driving Cellular Automaton Model 被引量:3
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作者 GEHong-Xia DAIShi-Qiang DONGLi-Yun LEILi 《Communications in Theoretical Physics》 SCIE CAS CSCD 2005年第2期321-324,共4页
A modified cellular automaton model for traffic flow on highway is proposed with a novel concept about the variable security gap. The concept is first introduced into the original Nagel-Schreckenberg model, which is c... A modified cellular automaton model for traffic flow on highway is proposed with a novel concept about the variable security gap. The concept is first introduced into the original Nagel-Schreckenberg model, which is called the non-sensitive driving cellular automaton model. And then it is incorporated with a sensitive driving NaSch model,in which the randomization brake is arranged before the deterministic deceleration. A parameter related to the variable security gap is determined through simulation. Comparison of the simulation results indicates that the variable security gap has different influence on the two models. The fundamental diagram obtained by simulation with the modified sensitive driving NaSch model shows that the maximumflow are in good agreement with the observed data, indicating that the presented model is more reasonable and realistic. 展开更多
关键词 traffic flow cellular automaton model sensitive behavior variable securitygap
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