生物微晶玻璃在模拟细胞外液中界面产物研究

生物玻璃、生物微晶玻璃的生物活性是关系到其埴入人体后能否与活骨组织产生牢固化学组合的关键因素。因此,研究CaO—MgO—P2O5—SiO2系统生物微晶玻璃的生物活性、尤其是在模拟细胞外液中的生物活性,对于医学临床应用具有重要的现实意义。实验结果表明,随着A/W样品在模拟细胞外液中浸泡时间的增加,如图1所示。样品表面首先有絮状物生成、并逐渐增多、晶化为小颗粒状晶体,继而转变为较大颗粒晶体覆盖层。相应的能谱分析（图2）表明,样品表面的Si元素成分逐渐减少,Ca元素成分则逐渐增加。

城市生活性物流设施网络实证研究

本文使用圆通快递运单数据，利用统计分析的方法，分析了圆通快递在全国的设施网络布局与网络流特征。圆通快递运输呈现转运中心与营业网点两个层级的集聚与分散。对于转运中心之间的运输，当货物途径两个转运中心时，得出转运中心之间货物运输量；当货物途径三个转运中心时，得出转运中心之间的中转网络。对于转运中心分拔至营业网点的过程，得出每个转运中心的分拔范围与货物分拔的空间分布，发现不同地区的转运中心分拨特征呈现较大的差异。此外，计算货物在运输过程各个阶段的时间花费，并按转运中心计算均值，发现转运中心分拨时间与转运中心的分布密度呈负相关，营业网点配送时间与营业网点的分布密度呈负相关。

Research progress on the application of ferulic acid in cosmetics

Ferulic acid is a phenolic compound widely exists in plants.Currently ferulic acid on the market is mainly extracted from plants,but it can also be obtained via biosynthesis or chemical synthesis.The biosynthesis method has a great potential for future production of ferulic acid.Ferulic acid is frequently used as a whitening ingredient in cosmetics,since it reduces melanin production by competitively inhibiting tyrosinase activity.It also has strong antioxidant activity,including elimination of free radicals,inhibition of ROS production,and regulation of various signaling pathways and the activity of antioxidant enzymes.The anti-UV activity of ferulic acid makes it applicable in sunscreen cosmetics.It can absorb UV rays and inhibit UVB-induced matrix metalloproteinase MMP-2 and MMP-9 activities to attenuate the damage caused by UV radiation.Ferulic acid is also reported to display protective effects on human keratinocytes and human skin fibroblasts.In addition,it is found to have effective anti-aging effect,mainly through inhibiting the degradation of hyaluronic acid by reducing the activities of collagenase and hyaluronidase,and inducing the biosynthesis of pre-collagen and hyaluronic acid.Ferulic acid shows potential for the treatment of atopic dermatitis,psoriasis and other inflammatory diseases of the skin.Its anti-inflammatory effect results from the inhibition of multiple inflammatory factors and signaling pathways.Ferulic acid displays broad-spectrum antibacterial effect,by damaging the cell membranes of bacteria and fungi which leads to membrane leakage and cell death.Furthermore,ferulic acid can also promote skin healing and regeneration.However,the instability of ferulic acid limits its applications in cosmetics.

Investigation of bioactivity and biodegradability of Mg-bioceramic implants:An in vitro study for biomedical applications

In this study,Mg-based composites,by the addition of ZnO,Ca_(2)ZnSi_(2)O_(7),Ca_(2)MgSi_(2)O_(7),and CaSiO_(3)as bioactive agents,were fabricated using friction stir processing.The microstructure and in vitro assessment of bioactivity,biodegradation rate,and corrosion behavior of the resultant composites were investigated in simulated body fluid(SBF).The results showed that during the immersion of composites in SBF for 28 d,due to the release of Ca^(2+)and PO_(4)^(3-)ions,hydroxyapatite(HA)crystals with cauliflower shaped morphology were deposited on the surface of composites,confirming good bioactivity of composites.In addition,due to the uniform distribution of bioceramic powders throughout Mg matrix,grain refinement of the Mg matrix,and uniform redistribution of secondary phase particles,the polarization resistance increased,and the biodegradation rate of composites significantly reduced compared to monolithic Mg matrix.The polarization corrosion resistance of Mg-ZnO increased from 0.216 to 2.499 kΩ/cm^(2)compared to monolithic Mg alloy.Additionally,Mg-ZnO composite with the weight loss of 0.0217 g after 28 d immersion showed lower weight loss compared to other samples with increasing immersion time.Moreover,Mg-ZnO composite with the biodegradation rate of 37.71 mm/a exhibited lower biodegradation rate compared to other samples with increasing immersion time.

Integrative analysis of bone-formation associated genes and immune cell infiltration in osteoporosis, and the prediction of active ingredients in targeted traditional Chinese medicine

Objective To explore the differential expression and mechanisms of bone formation-related genes in osteoporosis(OP)leveraging bioinformatics and machine learning methodologies;and to predict the active ingredients of targeted traditional Chinese medicine(TCM)herbs.Methods The Gene Expression Omnibus(GEO)and GeneCards databases were employed to conduct a comprehensive screening of genes and disease-associated loci pertinent to the pathogenesis of OP.The R package was utilized as the analytical tool for the identification of differentially expressed genes.Least absolute shrinkage and selection operator(LASSO)logis-tic regression analysis and support vector machine-recursive feature elimination(SVM-RFE)algorithm were employed in defining the genetic signature specific to OP.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses for the selected pivotal genes were conducted.The cell-type identification by estimating rela-tive subsets of RNA transcripts(CIBERSORT)algorithm was leveraged to examine the infiltra-tion patterns of immune cells;with Spearman’s rank correlation analysis utilized to assess the relationship between the expression levels of the genes and the presence of immune cells.Coremine Medical Database was used to screen out potential TCM herbs for the treatment of OP.Comparative Toxicogenomics Database(CTD)was employed for forecasting the TCM ac-tive ingredients targeting the key genes.AutoDock Vina 1.2.2 and GROMACS 2020 softwares were employed to conclude analysis results;facilitating the exploration of binding affinity and conformational dynamics between the TCM active ingredients and their biological targets.Results Ten genes were identified by intersecting the results from the GEO and GeneCards databases.Through the application of LASSO regression and SVM-RFE algorithm;four piv-otal genes were selected:coat protein(CP);kallikrein 3(KLK3);polymeraseγ(POLG);and transient receptor potential vanilloid 4(TRPV4).GO and KEGG pathway enrichment analy-ses revealed that these trait genes were predominantly engaged in the regulation of defense response activation;maintenance of cellular metal ion balance;and the production of chemokine ligand 5.These genes were notably associated with signaling pathways such as ferroptosis;porphyrin metabolism;and base excision repair.Immune infiltration analysis showed that key genes were highly correlated with immune cells.Macrophage M0;M1;M2;and resting dendritic cell were significantly different between groups;and there were signifi-cant differences between different groups(P<0.05).The interaction counts of resveratrol;curcumin;and quercetin with KLK3 were 7;3;and 2;respectively.It shows that the interac-tions of resveratrol;curcumin;and quercetin with KLK3 were substantial.Molecular docking and molecular dynamics simulations further confirmed the robust binding affinity of these bioactive compounds to the target genes.Conclusion Pivotal genes including CP;KLK3;POLG;and TRPV4;exhibited commendable significant prognostic value;and played a crucial role in the diagnostic assessment of OP.Resveratrol;curcumin;and quercetin;natural compounds found in TCM;showed promise in their potential to effectively modulate the bone-forming gene KLK3.This study provides a sci-entific basis for the interpretation of the pathogenesis of OP and the development of clinical drugs.

Comparative Study of the Biological Activities of the Skin Secretions from Six Common Chinese Amphibians

Water soluble skin secretions of six common Chinese amphibians were studied for their biological and enzymatic activities.The skin secretions of Tylototriton verrucosus,Bombina maxima ,and Bufo andrewsi were found toxic to mice with the intraperitoneal LD 50 of 11 5?mg/kg,18 8?mg/kg,and 264?mg/kg,respectively.No acute lethal toxicities were observed for the skin secretions of Rana nigromaculata,Rana guentheri and Rana limnocharis in a dose up to 500?mg/kg.The lethal toxicities of the skin secretions of T verrucosus and B maxima to mice are in the same grade as those of Viperidae snake venoms.The toxic components in T verrucosus and B maxima skin secretions are the proteins with molecular weights ranging from 3 to 60?kDa.All the skin secretions had both proteolytic activity and trypsin inhibitory activity.The skin secretions from T verrucosus , B maxima and B andrewsi also displayed wide spectrum antimicrobial activity.On the other hand,the skin secretions from B andrewsi and B maxima showed cytotoxicity on human cancer cells.All the six samples had not significant effects on mammalian blood coagulation system.Phospholipase A 2 activity was only found in the skin secretions of T verrucosus .None of these skin secretions showed acetylcholine esterase activity.

Preparation of micro-nanostructure on titanium implants and its bioactivity

Surface modification of medical implants was considered as an effective method to improve the cellular behaviors and the integration of tissue onto materials. The micro-nanostructured surface on the titanium alloy was prepared by laser treatment and multiple acid etching. The surface morphologies of different titanium alloy substrates were characterized by scanning electron microscopy (SEM). The effects of micro-nanostructured surfaces on the cellular responses were investigated in vitro by observing hydroxyapatite formation, cell morphology and cell adhesion. The results indicate that the micro-sized structure promoted the adhesion and proliferation of cultured osteoblasts. Furthermore, the micro-nanostructured surface was more conducive to cell adhension stretching compared with the micro-structured surface. All results suggest that the micro-nanostructured surface improved the biocompatibility and integration of tissue onto titanium alloy implants.

Exploration for a Natural Selenium Supplement——Characterization and Bioactivities of Se-containing Polysaccharide from Garlic

A Se—containing polysaccharide(Se-GPS),with average molecular weight of 1.5×10~4 was isolated from Hubei Enshi garlic(Allium sativum L.)and purified by means of Sephadex G25 to G200 chromatographies.Its chemical homogeneity and composition were studied by optical rotation,HPLC and paper chromatographical methods.The abilities of scavenging active oxygen free radicals and inhibiting the damage of erythrocyte membrane by SiO_2 were established by spin trapping ESR studies.Se-GPS was shown to inhibit human cytomegalovirus by plague reduction assays and cytopathogenic effect tests.

Soil properties in reclaimed farmland by filling subsidence basin due to underground coal mining with mineral wastes in China

Reclaimed mining-induced subsidence area soils (RMSs) could restore soil quality and crop productivity in coal mining area. This study was conducted to evaluate the effects of mineral-processing wastes (fly ash vs coal gangue) as backfill substrates on soil chemical and microbial properties in mining-induced subsidence area. A general higher water holding capacity (WHC) and pH had been observed in fly ash than coal gangue reconstructed soil. Soil microbial biomass C (MBC) and N (MBN), MBC/TOC (total organic carbon) ratio (qmic) were higher under the influence of the fly ash, while contents of As, Cr, C/Nbio, the basal respiration per unit of microbial biomass (QCO2) were higher under the coal gangue reconstructed mode in 0-10, 10-20, 20-50 cm layers. The microbial basal respiration was higher in 0-10, 10-20, 0-50 cm layers, while was lower in 20-50 cm layer under fly ash than that of coal gangue reconstructed mode. The lower QCO2 of fly ash mine soil suggested the lower maintenance energy requirement of the microbial community. Moreover, the contents of metals may possibly have negative implications for soil microbial and enzyme activities in reconstructed soil.

Herpetin, a New Bioactive Lignan Isolated from Herpetospermum caudigerum

Aim To study the chemical constituents of seeds of Herpetospermum caudigerum. Methods Column chromatography was used in the isolation procedure, and the structure was elucidated by spectral data, whose pharmacologic activity was assayed in vitro. Results A new compound named herpetin was isolated, whose structure was determined to be 3-benzofuran methanol-2, 3-dihydm-2-（4- hydroxy-3-methoxyphenyl ）-4-methoxy-6-[ tetrahydm-2-（ 3-hydroxy-4-methoxyphenyl ）-3-methanol ]-2- furanmethyl, showed significant inhibitory effects on HBV-DNA and the replication and expression of HBsAg and HBeAg. Conclusion Herpetin offers wide research and development prospect.

Functional evaluation for effective compositions in seed oil of Korean pine

The effect of seed oil of Korean pine (Pinus koraiensis) on the rats' blood-fat and its anti-ageing function was stud-ied for appraising the efficacy of the seed oil of Korean pine. Sixty experimental rats were randomly divided into 5 groups (half males and half females in each group) as normal control group, high fat diet control group, and three groups (Group 1 Group 2, Group 3) that were fed with feedstuff with the contents of the seed oil of 2.0g/(kgd-1), 4.0g/(kgd-1) and 8.0g/(kgd-1), respec-tively. The indexes such as superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and anti-oxidation capacity (AOC) were measured by Reagent Kit method. It was found that the seed oil of the Korean pine could reduce the content of triglyc-eride and improve SOD as well as GSH-PX activity in serum. These indexes of the rats in Group 2 fed with Korean pine seed oil of 4.0 g/(kgd-1) reached the significant level and those of rats in Group 3 fed with the seed oil of 8.0 g/(kgd-1) reached the extremely significant level. The results indicated the seed oil of Korean pine had function of regulating the level of blood-fat and anti-ageing.

A Bioactive Diterpene from the Chinese Herb Aster souliei

Aster souliei Franch, (Compositae) is a. herbaceous plant distributed innorth China. It has been used in folk medicine as antipyretic, detoxicant, expectorant andantitussive. In an effort to find biologically active components from Chinese medicinal plants ' ,we have examined the aerial parts of this herb, leading to the isolation of a clerodane-typediterpene, 18, 19-dihydroxy-5α, 10β-neo-cleroda-3, 13 (l4)-dien-16, 15-butenolide (1). In thispaper we report the structural elucidation, and the antitumor and antibacterial activities of thiscompound. It was found that 1 possesses moderate cytotoxicity against human leukemia cells (HL-60)and activity against microorganisms.

Modulation of Lianbizi Injection (Andrographolide ) on Some Immune Functions

Objective: To study the effects of andrographolide on immune functions and the immune mechanism in clinical therapy.Methods: The amounts of IFN-α,IFN-γ, TNF-α, IL-8 in the peripheral blood mononuclear cells (PBMC) culture supernatants dealt with by different concentrations of LianBiZhi (LBZ) injection, the effective component of which is andrographolide, were detected by biological activity test or ELISA in vitro. The effects of LBZ injection on macrophage phagocytotic function and natural killer cells cytotoxicity were examined by means of macrophage to phagocytize cock erythrocyte and measurement of lactate dehydrogenase (LDH) activity released from the damaged cells, respectively.Results: The LBZ injection could not only enhance the phagocytosis activity of peritoneal macrophage from guinea pig to phagocytosis cock erythrocytes, but also augment the cytotoxicity mediated by natural killer cells from PBMCs.Conclusion: Andrographolide is an immunostimulant agent which can modulate both antigen specific and nonspecific immune function by means of its natural killer cells, macrophage and cytokines.

Influence of Pyrolytic Biochar on Settleability and Denitrification of Activated Sludge Process

Biochar is a massively produced by-product of biomass pyrolysis to obtain renewable energy and has not been fully used. Incomplete separation of sludge and effluent and insufficient denitrification of sewage are two of main factors that influence the efficiency of activated sludge process. In this work, we proposed a new utilization of biochar and investigated the effect of biochar addition on the performance of settleability and denitrification of activated sludge. Results show that the addition of biochar can improve the settleability of activated sludge by changing the physicochemical characteristics of sludge （e.g., flocculating ability, zeta-potential, hydrophobicity, and extracellular polymeric substances constituents）. Moreover, the dissolved organic carbon released from biochar obtained at lower pyrolysis temperature can improve the nitrate removal efficiency to a certain extent.

Synthesis and Insecticidal Activity of Derivants of 3,13-Ester Mesaconitine

[Objective] The experiment aimed to explore the relations between structure of aconitine, aconitine derivatives and insecticide. [Method] The aconitine derivatives such as 3-acetyl mesaconitine, 3-propionyl mesaconitine, 3-acetyl-13-Benzoyl-mesaconitine and 3-propionyl-13-Benzoyl-mesaconitine were synthesized by mesaconitine with acetic anhydride or propionic anhydride and their insecticidal activities were also determined. [Result] When the concentration was 500 mg/L, the insecticidal activities of mesaconitine against Nilaparvata legen and Aphis were 50% and 30% respectively while the insecticidal activities of 3-acetyl mesaconitine, 3-propionyl mesaconitine, 3-acetyl-13-Benzoyl-mesaconitine and 3-propionyl-13-Benzoyl-mesaconitine against Aphis medicagini were 40%, 30%, 30% and 20% respectively at 500 mg/L. [Conclusion] After hydroxyl esterification, the insecticidal activity of mesaconitine was declined and the existence of hydroxyl at 3rd position in mesaconitine played very important influences on insecticidal activity.

Effect of Protective Cultivation Patterns of Rice in Cold Areas on Soil Physiological and Biochemical Status in Paddy Field

[Objective] The research aimed to explore how to use the soil reasonably,prevent the degradation of soil fertility,maintain soil fertility,improve the ecological environment of paddy field and improve the soil productivity of paddy field from the cultivation aspect.[Method] Taking kenjiandao 10 as the material,the variation laws of root weight,soil physical and chemical characteristics,soil enzyme,straw decomposition rate,soil temperature,microorganism of rice under the planting patterns of water-saving pro...

Bioactive Characteristics of Soil Microorganisms in Different-aged Orange (Citrus reticulate) Plantations

[Objective] The paper was to study the bioactive characteristics of soil microorganisms in different-aged orange plantations. [Method] Taking 010 cm deep soil in 3 orange plantations with different planting years in suburb of Yichang City as the test object, the variation rule of total organic carbon, total nitrogen, soil pH value, microbial biomass carbon, microbial biomass nitrogen, number of 3 main types of soil microbial flora, basal respiration, microbial entropy and metabolic entropy in differentaged orange plantations was studied. [Result] With the increase of planting years, the soil acidification of different-aged orange plantations was aggravating; total organic carbon and total nitrogen content increased first and then decreased; the total number of soil microorganism showed a downtrend, of which the number of bacteria decreased significantly, the number of actinomycetes had small changes, the number of fungi increased significantly, and the ratio of bacteria and fungi in soil （B/F） showed a decreasing trend. Soil microbial biomass carbon was fluctuated within a small range, whereas soil microbial biomass nitrogen decreased significantly; soil microbial entropy decreased significantly, and metabolic entropy showed an increasing trend. This indicated that the decrease of soil pH value affected the changes of soil microbial flora, microbial activity, microbial biomass carbon and nitrogen and soil major nutrients, and further affected the normal exertion of soil function. [Conclusion] The study explores soil nutrient characteristics and changes of microbial flora in test area, which will provide scientific basis for further study on orchard soil and orchard management.

Soil vanadium pollution and microbial response characteristics from stone coal smelting district

A field investigation was performed to study the content, speciation and mobility of vanadium, as well as microbial response in soil from a stone coal smelting district in Hunan province of China. The results showed that the contents of soil V ranged from 168 to 1538 mg/kg, which exceeded the maximum permissible value of Canadian soil quality for V. The mean soil V content from wasteland area reached 1421 mg/kg, and those from the areas related with slag heap, ore pile and smelting center were 380, 260 and 225 mg/kg, respectively. Based on the results of the modified BCR sequential extraction procedure, V contents in the mobile fractions varied from 19.2 to 637 mg/kg accounting for 7.4%-42.3% of total V, and those of V(+5) species were between 21.9 and 534.0 mg/kg. Soil enzyme activity and microbial basal respiration were adversely affected by high level of soil V. More attention should be paid to soil V pollution and potential hazardous surrounding the stone coal smelting district.

Effects of Different Fertilization Treatments on Soil Microbial Biomass,Soil Enzyme Activities and Related Nutrients in Continuous-cropping Sugarcane Field

[Objective] This study was conducted to expound the fertility improvement effect in continuous-cropping sugarcane field and provide reference for establishment of rational sugarcane fertilization system and improvement of soil quality in continuous-cropping sugarcane field. [Method] The soil in the experimental region is latosolic red soil which was planted with sugarcane for 11 years continuously, and 8 treatments including sole application of chemical fertilizers, sole application of organ- ic fertilizer, and combined application of organic fertilizer and chemical fertilizers were designed according to different fertilization measures. The effects of different fertilization treatments on soil microbial biomass, soil enzyme activities and related fertility factors were determined. [Result} Different fertilization treatments all showed soil microbial biomass N, C and P and activities of soil acid phosphatase, catalase, sucrase and urease higher than the CK. Soil microbial biomass N increased by 5.56%-67.13%, soil microbial biomass C increased by 4.01%-20.40%, and soil mi- crobial biomass P increased by 6.39%-67.02%. The activity of acid phosphatase was improved by 12.96%-35.19%, the activity of catalase was improved by 18.24% -78.93%, the activity of sucrase was improved by 3.00%-42.00%, and the activity of urease was improved by 1.21%-23.43%. However, the soil nutrients of different fertilization treatments increased non-significantly （P〉0.05）. Soil microbial biomass N, C and P and activities of acid phosphatase, catalase and urease were in significant （P〈0.05） or very significant correlation （P〈0.01） with contents of soil rapidly available P, rapidly available K and total N. [Conclusion] The evaluation of improvement of soil fertility in continuous-cropping sugarcane field using soil microbial biomass and enzyme activities as indexes is more comprehensive and sensitive.

Effects of Different Land Use Patterns on Soil Microbial Quantity and Activity in Hongta District,Yuxi City

[Objective] This study aimed to investigate the effects of different land use patterns on soil ecological environment. [Method] Total three representative land use patterns （corn field, cherry tree land, wood land） were selected from Hongta District, Yuxi City, and under these three patterns, soil microbial quantity and activity were studied. [Result] Under the three land use patterns, soil microorganisms were domi- nated by bacteria; soil microbial quantity ranked as wood land＇s〉cheery tree land＇s〉 corn field＇s; and total microbial activity, catalase activity and urease activity all ranked as cherry tree land＇s〉wood land＇s〉corn field＇s. [Conclusion] Soil microbial activity and functions are related to farmland management measures, as well as land use pattern and soil nutrients.