The high phenotypic plasticity in the shell of oysters presents a challenge during taxonomic and phylogenetie studies of these economically important bivalves. However, because DNA can exhibit marked differences among...The high phenotypic plasticity in the shell of oysters presents a challenge during taxonomic and phylogenetie studies of these economically important bivalves. However, because DNA can exhibit marked differences among morphologically similar species, DNA barcoding offers a potential means for oyster identification. We analyzed the complete sequences of the cytochrome oxidase subunit I （COI） of five common Crassostrea species in China （including Hong Kong oyster C. hongkongensis, Jinjiang oyster C. ariakensis, Portuguese oyster C. angulata, Kumamoto oyster C. sikamea, and Pacific oyster C. gigas） and screened for distinct fragments. Using these distinct fragments on a high-resolution melting analysis platform, we developed an identification method that does not rely on species-specific PCR or fragment length polymorphism and is efficient, reliable, and easy to visualize. Using a single pair of primers （Oyster- COI-1）, we were able to successfully distinguish among the five oyster species. This new method provides a simple and powerful tool for the identification of oyster species.展开更多
Knowledge about climate change impacts on species distribution at national scale is critical to biodi- versity conservation and design of management programs. Although China is a biodiversity hot spot in the world, po...Knowledge about climate change impacts on species distribution at national scale is critical to biodi- versity conservation and design of management programs. Although China is a biodiversity hot spot in the world, potential influence of climate change on Chinese protected birds is rarely studied. Here, we assess the impact of climate change on 108 protected bird species and nature reserves using species distribution modeling at a relatively fine spatial resolution （1 km） for the first time. We found that a large proportion of protected species would have potential suitable habitat shrink and northward range shift by 77-90 km in response to projected future climate change in 2080. Southeastern China would suffer from losing climate suitability, whereas the climate conditions in Qinghai-Tibet Plateau and northeastern China were projected to become suitable for more protected species. On average, each protected area in decline of suitable climate for China would experience a 3-4 species by 2080. Cli- mate change will modify which species each protected area will be suitable for. Our results showed that the risk of extinction for Chinese protected birds would be high, even in the moderate climate change scenario. These findings indicate that the management and design of nature reserves in China must take climate change into consideration.展开更多
基金Supported by the National Basic Research Program of China(973 Program)(No.2010CB126402)the National Natural Science Foundation of China(Nos.40730845,41206149)+4 种基金the Shandong Provincial Natural Science Foundation(No.ZR2010DQ024)the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A405)the Earmarked Fund for Modern Agro-Industry Technology Research System(No.CARS-48)the Taishan Scholar Program of Shandong Provincethe Taishan Scholar Climbing Program of Shandong Province
文摘The high phenotypic plasticity in the shell of oysters presents a challenge during taxonomic and phylogenetie studies of these economically important bivalves. However, because DNA can exhibit marked differences among morphologically similar species, DNA barcoding offers a potential means for oyster identification. We analyzed the complete sequences of the cytochrome oxidase subunit I （COI） of five common Crassostrea species in China （including Hong Kong oyster C. hongkongensis, Jinjiang oyster C. ariakensis, Portuguese oyster C. angulata, Kumamoto oyster C. sikamea, and Pacific oyster C. gigas） and screened for distinct fragments. Using these distinct fragments on a high-resolution melting analysis platform, we developed an identification method that does not rely on species-specific PCR or fragment length polymorphism and is efficient, reliable, and easy to visualize. Using a single pair of primers （Oyster- COI-1）, we were able to successfully distinguish among the five oyster species. This new method provides a simple and powerful tool for the identification of oyster species.
基金supported by the National High Technology Research and Development Program of China(‘‘863’’Program)(2009AA12200101)the National Natural Science Foundation of China(41471347)
文摘Knowledge about climate change impacts on species distribution at national scale is critical to biodi- versity conservation and design of management programs. Although China is a biodiversity hot spot in the world, potential influence of climate change on Chinese protected birds is rarely studied. Here, we assess the impact of climate change on 108 protected bird species and nature reserves using species distribution modeling at a relatively fine spatial resolution （1 km） for the first time. We found that a large proportion of protected species would have potential suitable habitat shrink and northward range shift by 77-90 km in response to projected future climate change in 2080. Southeastern China would suffer from losing climate suitability, whereas the climate conditions in Qinghai-Tibet Plateau and northeastern China were projected to become suitable for more protected species. On average, each protected area in decline of suitable climate for China would experience a 3-4 species by 2080. Cli- mate change will modify which species each protected area will be suitable for. Our results showed that the risk of extinction for Chinese protected birds would be high, even in the moderate climate change scenario. These findings indicate that the management and design of nature reserves in China must take climate change into consideration.
