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植物DNA混合物中物种成分实时荧光PCR定量分析
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作者 杨永存 杨冬燕 +6 位作者 王学林 杨小柯 周向阳 郭良让 林健荣 邓平建 吴水清 《中国卫生检验杂志》 CAS 2009年第6期1206-1209,共4页
目的:建立植物DNA混合物中物种成分实时荧光PCR定量方法。方法:建立PCR反应管中某物种DNA质量和该物种特异性基因扩增Ct值之间的定量关系,通过260 nm处光密度值的测定计算DNA提取液的浓度和DNA总质量,并以某物种DNA和总DNA的质量百分... 目的:建立植物DNA混合物中物种成分实时荧光PCR定量方法。方法:建立PCR反应管中某物种DNA质量和该物种特异性基因扩增Ct值之间的定量关系,通过260 nm处光密度值的测定计算DNA提取液的浓度和DNA总质量,并以某物种DNA和总DNA的质量百分比表示该物种DNA含量。对56个待测样品中的物种成分进行定量测定以验证新建方法的准确性和精密度。结果:新建方法的LOQ低于0.51%;B ias为0.00%~20.79%,RSD为0.43%~19.64%。结论:新建方法的准确度和精密度均符合现行要求。 展开更多
关键词 DNA混合物 物种定量 实时荧光PCR
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Stability of Mbuna Species Populations in Lake Malawi
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作者 Lucious G. Kanyumba Wisdom J. Changadeya +2 位作者 Aggrey J.D. Ambali Leonard A. Kamwanja EmmanuelK.W. Kaunda 《Journal of Environmental Science and Engineering(B)》 2012年第4期543-555,共13页
The first comprehensive study of abundance, richness and distribution of Mbuna in Lake Malawi was carried out in the 1980s. The present study examined changes of species richness and abundance of Mbuna with time. Most... The first comprehensive study of abundance, richness and distribution of Mbuna in Lake Malawi was carried out in the 1980s. The present study examined changes of species richness and abundance of Mbuna with time. Most sites demarcated during the 1980s survey were explored in order to determine changes in abundance and composition of Mbuna over a period of more than 20 years Chi-square analysis revealed significant difference (p 〈 0.05) in abundance of Mbuna between 1983 and 2005 with the exception of Chinyankhwazi and Chinyamwezi where the difference was not significant (p 〉 0.05). Cluster analysis showed changes in species composition between 1983 and 2005 in various sites. Generally, there were more species lost in a given area than new species observed in 2005. Decline in Mbuna species richness might have been caused by siltation, harvesting of Mbuna for food and ornamental fish trade while new species were mostly introduced through translocation from one part of the lake to another. 展开更多
关键词 Mbuna species abundance species richness translocation.
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Identification of variety of rice and determination of content of each rice in mixtures of two rices by nonlinear chemical analysis
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作者 CHEN Chun-nan XU Tian-shu +9 位作者 ZHONG Jun-hui WANG Zhi-pei LUO Xue-lei GUO Fang-qiu ZHANG Tai-ming DU Juan YANG Chun-hua ZHU Hong-qiu HUANG Jian DENG Fei-yue 《Journal of Central South University》 SCIE EI CAS CSCD 2018年第2期251-258,共8页
Fingerprints of two varieties of rice and their mixtures were investigated by a nonlinear chemical reaction system consisting of rice components,sodium bromate,manganese sulfate,sulfuric acid and acetone.The variety o... Fingerprints of two varieties of rice and their mixtures were investigated by a nonlinear chemical reaction system consisting of rice components,sodium bromate,manganese sulfate,sulfuric acid and acetone.The variety of rice was identified by the visual characteristic of fingerprint and system similarity pattern recognition,and the content of each variety of rice in the mixture was determined by the quantitative information of fingerprint.The results show that nonlinear chemical analysis may be used to exactly identify the variety of pure rice and to accurately determine the content of each variety of rice in the mixture,indicating the method is simple and convenient. 展开更多
关键词 nonlinear chemical analysis binary mixture variety identification content determination RICE
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