电针结合穴位注射恩再适对急性亚急性非特异性腰痛的临床研究

目的:观察电针结合穴位注射牛痘疫苗致炎兔皮提取物(恩再适)对急性亚急性非特异性腰痛的临床疗效与疗程。方法:将113例急性亚急性非特异腰痛患者随机分为电针组56例和电针穴注组57例,电针组采用单纯电针治疗,电针穴注组采用电针结合穴位注射治疗。治疗前后进行疼痛视觉模拟评分(visual analogus scale,VAS),记录治疗次数及评价临床疗效。结果:两组患者治疗后VAS疼痛评分均显著低于治疗前(P<0.01),电针穴注组治疗次数显著低于电针组(P<0.01)。结论:电针结合牛痘疫苗致炎兔皮提取物穴位注射,能快速、安全、有效地减轻急性亚急性非特异性腰痛的症状。

牛痘疫苗致炎兔皮肤提取物的体外抗肿瘤活性

目的探讨牛痘疫苗致炎兔的皮肤提取物注射液(恩再适)体外对人源肿瘤细胞增殖的影响及对体外小鼠脾淋巴细胞分泌细胞因子的作用。方法以5种人肿瘤细胞HepG2、LM3、H460、A549和HeLa为受试对象,利用CCK-8法检测恩再适(1.63、0.815、0.326、0.163和0.0815 U/ml)对细胞增殖的影响;无菌分离小鼠脾,制备脾淋巴细胞悬液并用含10%胎牛血清(FBS)的RPMI-1640培养基培养,加入刺激剂刀豆蛋白(ConA)或脂多糖(LPS),以及不同浓度的恩再适(0.815、0.163和0.0815 U/ml)共培养24 h,ELISA试剂盒检测上清液中白细胞介素-2(IL-2)、干扰素-γ(IFN-γ)、IL-4和IL-12的分泌水平;恩再适0.163 U/ml处理脾淋巴细胞24 h,取上清液原液、与新鲜培养基不同比例的稀释液,培养HepG2细胞24 h,CCK-8法检测细胞增殖活性。结果随恩再适浓度的增加,5种肿瘤细胞的抑制率逐渐增加,呈剂量依赖关系;当该药浓度为1.63 U/ml时,对HepG2、LM3、H460、A549和HeLa细胞的抑制率分别达到58.95%、55.08%、57.28%、45.80%和48.18%。ELISA结果显示,与对照组相比,恩再适0.163和0.815 U/ml组小鼠脾淋巴细胞的IL-2、IFN-γ和IL-4分泌水平显著提高(P<0.01),恩再适0.0815、0.163和0.815 U/ml组的IL-12分泌水平也均显著提高(P<0.01)。经用恩再适0.163 U/ml处理小鼠脾淋巴细胞24 h所得上清液对HepG2细胞增殖呈剂量依赖型抑制活性,上清液原液对HepG2细胞的抑制作用较恩再适0.163 U/ml处理组相比,显著增强(P<0.01)。结论恩再适具有体外抑制5种人源肿瘤细胞增殖的作用,上调体外培养小鼠淋巴细胞的IL-2、IFN-γ、IL-4和IL-12等细胞因子分泌水平,该效应可能与其抑制HepG2细胞增殖有关。

电员针刺激联合恩再适治疗肩周关节炎

肩周关节炎是一种退行性炎症病变,主要发生部位为肩部关节囊和肩关节周围。临床患者以中老年居多,其中多在50岁左右,有“五十肩”之称[1]。临床上肩周关节炎患者常因为肩关节周围的软组织无菌性炎症而出现疼痛及肩部活动功能障碍的症状,对患者的生活、工作带来了诸多不便。现将笔者自2012年03月起对肩周关节炎患者进行电员针刺激联合恩再适静脉滴注治疗情况总结报告如下。

Tomato detection method using domain adaptive learning for dense planting environments

This study aimed to address the challenge of accurately and reliably detecting tomatoes in dense planting environments,a critical prerequisite for the automation implementation of robotic harvesting.However,the heavy reliance on extensive manually annotated datasets for training deep learning models still poses significant limitations to their application in real-world agricultural production environments.To overcome these limitations,we employed domain adaptive learning approach combined with the YOLOv5 model to develop a novel tomato detection model called as TDA-YOLO(tomato detection domain adaptation).We designated the normal illumination scenes in dense planting environments as the source domain and utilized various other illumination scenes as the target domain.To construct bridge mechanism between source and target domains,neural preset for color style transfer is introduced to generate a pseudo-dataset,which served to deal with domain discrepancy.Furthermore,this study combines the semi-supervised learning method to enable the model to extract domain-invariant features more fully,and uses knowledge distillation to improve the model's ability to adapt to the target domain.Additionally,for purpose of promoting inference speed and low computational demand,the lightweight FasterNet network was integrated into the YOLOv5's C3 module,creating a modified C3_Faster module.The experimental results demonstrated that the proposed TDA-YOLO model significantly outperformed original YOLOv5s model,achieving a mAP(mean average precision)of 96.80%for tomato detection across diverse scenarios in dense planting environments,increasing by 7.19 percentage points;Compared with the latest YOLOv8 and YOLOv9,it is also 2.17 and 1.19 percentage points higher,respectively.The model's average detection time per image was an impressive 15 milliseconds,with a FLOPs(floating point operations per second)count of 13.8 G.After acceleration processing,the detection accuracy of the TDA-YOLO model on the Jetson Xavier NX development board is 90.95%,the mAP value is 91.35%,and the detection time of each image is 21 ms,which can still meet the requirements of real-time detection of tomatoes in dense planting environment.The experimental results show that the proposed TDA-YOLO model can accurately and quickly detect tomatoes in dense planting environment,and at the same time avoid the use of a large number of annotated data,which provides technical support for the development of automatic harvesting systems for tomatoes and other fruits.

Study on the Introduction of Plants with Colored Foliage in Zhengzhou Area and Their Adaptability

[Objective] This study aimed to preliminarily select the plants with colored foliage adaptable to Zhengzhou area. [Method] An introduction trial on 42 varieties with colored foliage was conducted, in which major ornamental characteristics, phenological phases and stress resistance of all the introduced plants with colored foliage were observed and the introduced plants were comprehensively evaluated. [Result] Golden-and Compound-leaved Maple, Red-leaved Sakura, Pink-and Compound-leaved Maple, Amur Maple, Purple-leaved Canadian Redbud with good performance were selected preliminarily, which can be applied for landscape in Zhengzhou area. Among them, Golden- and Compound-leaved Maple, Golden- and Silvery-leaved Maple and Purple-leaved Smoketree can either be planted separately, or in a line as boulevard trees, or in groups as ornamental forest; Red-leaved Sakura, Pink- and Compound-leaved Maple and Purple-leaved Canadian Redbud can be planted in groups or separately in gardens, parks or alongside streets or in front of buildings; Amur Maple with high adaptability is a excellent tree species for afforesting barren mountain. [Conclusion] This study has not only provided new excellent germplasm sources for the landscaping in Zhengzhou area, thus favoring the improvement of local landscaping level, but also provides examples and references for the introduction of plants with colored foliage in the other areas.

预反应态模型浅析:催化活性和近过渡态分子模拟

当今生物合成催化元件超进化分子理性设计的瓶颈在于有限的计算资源、研究时间与催化反应复杂势能面接近无穷无尽的计算需求之间的矛盾。然而,两个前所未有的数据集合有望拓新蛋白质工程人工智能化分子设计,其一是高通量定向进化实验带来的巨量高效突变体序列信息,其二是基于结构生物学的高阶量子力学计算所揭示的全原子飞秒精度反应机制。本文从催化基本理论、米氏复合物近进攻构象、催化循环效率控制点的角度浅析预反应态模型的基本概念和应用。预反应态模型尝试利用在低反应势垒生物化学反应中内禀的近进攻构象与过渡态具有相近的物理化学稳定性,弹性地选择与催化元件进化目标相关的关键过渡态,利用经典分子动力学模拟分析近过渡态的活性构象布居数与远端突变、底物结构、实验条件的关系。预反应态分析的基本流程为:首先,基于高阶量子力学反应势能面提取催化中心关键过渡态的结构特征;其次,从高精度蛋白质三维结构出发,结合氨基酸质子化生物信息学预测工具构建出关键过渡态对应的近进攻态活性构象;最后,利用过渡态结构特征设定分子动力学模拟初始约束条件,并逐步取消约束条件测试预反应态随氨基酸突变和底物变化的稳定性变化,以近进攻构象在预反应态轨迹中布居数作为“预反应态-酶活”半定量相关系数,从预反应态稳定性中挖掘酶与底物的适配图谱。当前在预反应态动态结构与酶活的定量关系分析上还有诸多难题亟待突破,利用高通量高阶量子化学再采样计算、结合机器学习人工智能分析代表了预反应态模型的发展方向。

Organic Acids Secreted from Plant Roots under Soil Stress and Their Effects on Ecological Adaptability of Plants

[Objective] In this study,the secretion of organic acids from plant roots under soil nutrient and water stress and the effects of organic acids on ecological adaptability of plants were investigated,which provided theoretical basis for improving the adaptability of plants to a variety of stress conditions.The results showed that,under nutrient and water stress,the content of organic acids secreted from plant roots increased significantly as a common active adaptive response.Organic acids could improve the activities of a variety of antioxidant enzymes,contents of osmotic regulatory substances,contents of chlorophyll and photosynthesis levels,promote nutrient absorption and transportation in plants,and ultimately contribute to plant growth and biomass accumulation,reduce the toxicity of stress conditions to plants and improve the stress resistance and adaptability of plants.

《庄子·逍遥游》中的和谐思想

以《庄子》"内篇"开篇的逍遥游为阐述基础,主要论述庄子在追求精神自由的过程中,他思想的发展过程,并且会与《庄子》"杂篇"中的思想片段相印证。本篇论文首先从《庄子》追求阔大恢宏的自由境界出发,逐步深入,依次论述庄子思想过程的思想阶段:"小大之辨"与"物适其性"和坐忘,但到这里并不是终结。最后,讨论一下《庄子·逍遥游》的和谐思想。

Study on the Floating Plant Climatic Adaptation of Undercurrent Constructed Wetland in North China

[Objective] The aim was to choose appropriate floating plant in the wetland in the north China.[Method] Pistia stratiotes L.,Eichhornia crassipes,and Hydrocharis dubia（Bl.） Backer were planted in the aquatic biological pool of constructed subsurface flow wetland system in the reservoir.Through filed investigation,the growth of the three kinds of plants was studied and their adaptability to the northern climate was concluded.[Result] Judging from the growth speed and state of the three kinds of floating plants,the biological characteristic of Pistia stratiotes L.can perfectly adapt the environment in the pool in the reservoir,followed by the Eichhornia crassipes.The growth state of the Hydrocharis dubia（Bl.） Backer was the worst one and it can not adapt to the north environment.[Conclusion] It provided references for the choice of artificial floating plant in the north area.

军事工程建设和谐管理的理论架构与实现模式

从军事工程建设区别于民用工程建设的特点出发,提出了军事工程建设和谐管理的背景。以组织行为学、军事工程经济学、现代项目管理学、系统协同进化理论为理论依据,并从军事工程建设和谐管理的本体论、认识论、方法论出发,描述了军事工程建设和谐管理的"人—事—物"逻辑关系及其系统架构,以及军事工程建设和谐管理的运行机理,从而建立了军事工程建设和谐管理的理论架构。从"领导—员工—团队"主体要素的人理和谐、"质量—工期—成本"客体要素的物理和谐、"知识—工具—方法"关系要素的事理和谐、"军事—技术—经济"系统环境和谐4个方面探讨了军事工程建设和谐管理的实现模式,并构建了军事工程建设和谐管理模式的评价模型。

Vibration control of pedestrian-bridge vertical dynamic coupling interaction based on biodynamic model

The human-induced vertical vibration serviceability of low-frequency and lightweight footbridges is studied based on the moving mass-spring-damper（MMSD） biodynamic model, and the mass damper（TMD） with different optimal model parameters being used to control the vertical vibration.First, the MMSD biodynamic model is employed to simulate the pedestrians, and the time-varying control equations of the vertical dynamic coupling system of the pedestrian-bridgeTMD are established with the consideration of pedestrianbridge dynamic interaction; and the equations are solved by using the Runge-Kutta-Felhberg integral method with variable step size. Secondly, the footbridge dynamic response is calculated under the model of pedestrian-structure dynamic interaction and the model of moving load when the pedestrian pace frequency is consistent with the natural frequency of footbridge. Finally, a comparative study and analysis are made on the control effects of the vertical dynamic coupling system in different optimal models of the TMD. The calculation results show that the pedestrian-bridge dynamic interaction cannot be ignored when the vertical human-induced vibration serviceability of low-frequency and light-weight footbridge is evaluated. The TMD can effectively reduce the vibration under the resonance of pedestrian-bridge, and TMD parameters are recommended for the determination by the Warburton optimization model.

Intraclonal Resource Sharing and Functional Specialization of Ramets in Response to Resource Heterogeneity in Three Stoloniferous Herbs

Environments with reciprocal patchiness of resources, in which the availability of two resources such as light and soil nutrients are patchily distributed in horizontal space and negatively correlated in each patch, are common in many ecosystems. The strategies by which clonal plants adapt to this type of heterogeneous environment were examined in three stoloniferous herbs,Potentilla reptans L. var. sericophylla Franch., P. anserina L. and Halerpestes ruthenica (Jacq.) Qvcz., commonly inhabiting forest understories, grasslands and low saline meadows, respectively. As pairs of connected ramets were subjected to reciprocal patchiness of light and nutrients, stolon connection between the two ramets significantly enhanced biomass of both ramet growing in low light intensity but high soil nutrient condition (LH ramet) and ramet growing in high light intensity but low soil nutrient condition (HL ramet) as well as whole ramet pairs (consisting of LH ramets and HL ramets). Additionally, stolon connection greatly increased root/shoot ratio of LH ramet while significantly decreased that of HL ramet. The results indicate that a reciprocal transportation of resources between interconnected ramets and a functional specialization of ramets in uptake of abundant resources occurred. By resource sharing and functional specialization, clonal plants can efficiently acquire locally abundant resources and buffer the stress caused by reciprocal patchiness of resources.

Self-adapting extraction of matrix mineral bulk modulus and verification of fluid substitution

Gassmann＇s equations are commonly used for predicting seismic wave velocity in rock physics research.However the input matrix mineral bulk modulus parameters are not accurate,which greatly influences the prediction reliability.In this paper,combining the Russell fluid factor with the Gassman-Biot-Geertsma equation and introducing the dry-rock Poisson＇s ratio,we propose an effective matrix mineral bulk modulus extraction method.This method can adaptively invert the equivalent matrix mineral bulk modulus to apply the Gassmann equation to fluid substitution of complex carbonate reservoirs and increase the fluid prediction reliability.The verification of the actual material fluid substitution also shows that this method is reliable,efficient,and adaptable.

浅谈泰州乔园独特的竹文化

指出了随着我国园林事业的迅猛发展,许多城市的公园建设出现了千变一律的现象。剖析了泰州乔园的竹文化布局和内涵,以供同行参考。

Modeling habitat suitability of range plant species using random forest method in arid mountainous rangelands

Mountainous rangelands play a pivotal role in providing forage resources for livestock, particularly in summer, and maintaining ecological balance. This study aimed to identify environmental variables affecting range plant species distribution, ecological analysis of the relationship between these variables and the distribution of plants, and to model and map the plant habitats suitability by the Random Forest Method(RFM) in rangelands of the Taftan Mountain, Sistan and Baluchestan Province, southeastern Iran. In order to determine the environmental variables and estimate the potential distribution of plant species, the presence points of plants were recorded by using systematic random sampling method(90 points of presence) and soils were sampled in 5 habitats by random method in 0–30 and 30–60 cm depths. The layers of environmental variables were prepared using the Kriging interpolation method and Geographic Information System facilities. The distribution of the plant habitats was finally modelled and mapped by the RFM. Continuous maps of the habitat suitability were converted to binary maps using Youden Index(?) in order to evaluate the accuracy of the RFM in estimation of the distribution of species potentialhabitat. Based on the values of the area under curve(AUC) statistics, accuracy of predictive models of all habitats was in good level. Investigating the agreement between the predicted map, generated by each model, and actual maps, generated from fieldmeasured data, of the plant habitats, was at a high level for all habitats, except for Amygdalus scoparia habitat. This study concluded that the RFM is a robust model to analyze the relationships between the distribution of plant species and environmental variables as well as to prepare potential distribution maps of plant habitats that are of higher priority for conservation on the local scale in arid mountainous rangelands.

The Biocompatibility of Wool Keratin

Keratin is the major structural fibrous protein providing outer covering such as hair,wool,feathers,etc.When being used as a kind of biomaterials,the biocompatibility of wool keratin is one of the most critical questions.By now,there has not been systemic study on the biocompatibility of keratin.Therefore,in this article we used the procedures of skin irritation,haemolysis and subcutaneous implantation according to ISO 10993 to study it.Moreover,the Fourier transform-infrared(FTIR) spectroscopy was utilized to analyse the impurity and structure modification of wool keratin film.The part of the animal tests showed that the wool keratin films prepared by authors were biocompatible.But the residual of sodium dodecyl sulfate(SDS) affected the results of other tests.Consequently,the wool keratin membrane is one kind of favourable and promising biomaterial for biomedical and histological utilization.The residual SDS used as an agent should be eliminated from the keratin solution or membrane completely if for biological usage.In conclusion,wool keratin,as a kind of natural protein,prospectively could be applied in biomedical materials and scaffolds of tissue engineering.

从“各适物宜”到“兴象”——中国古代意象论的核心环节

从“各适物宜”到“兴象”是中国古代意象论的两个核心环节。“各适物宜”论对“物”格外重视,是中国文学意象理论的发端期,意象理论的天平偏向于“象”之传神。“兴象”说,紧承“象其物宜”理论命题,亦特别重视对“言”、“意”之中间环节“象”的开掘。“兴象”的理论内涵至宋代严羽而成熟,元、明、清诸家“兴象玲珑”、“兴象深婉”的说法,都是对严羽的响应。“兴象玲珑”是指意象具有“玲珑”的象外之象;而“玲珑”之象又具有“深婉”的意外之意,二者合观之,当是严羽及后来的“兴象”论者的本义。

Cold acclimation induced accumulation of phenolic compounds and freezing tolerance in Ammopiptanthus mongolicus

Ammopiptanthus mongolicus, the only freezing tolerant evergreen broad-leaved shrub, local species of the Alashan desert, northwest sand area of China, can survive -30℃ or even lower temperature in winter. In the present study, the secondary products phenolics in A. mongolicus cotyledons were determined to study the effects of phenolics on cold tolerance. Cytochemical localization of phenolics in cotyledon cells was observed by electron microscopy and the content of phenolic compounds was assayed by spectrophotometric measurement. The results showed that the freezing tolerance ofA. mongolicus seedlings increased after acclimation at 2℃ for 14 days, which accompanied the increase of the content of phenolic compounds in cotyledons. Cytochemical observation showed that phenolic deposits were mainly localized in vacuoles and in close proximity to tonoplast, and also in the cytoplasm. The amount and the size of phenolics droplets increased obviously in cytoplasm and vacuoles after cold acclimation, predominantly aggregated along membranes of vacuoles and tonoplast. No phenolic deposits were found in cell walls. As hydrogen- or elec- tron-donating agents, phenolics may protect plant cells against reactive oxygen species formed during chilling or freezing stress and improve the freezing tolerance of cold-acclimated A. mongolicus seedlings.

Experimental study on interbody fusion with CFR/PEEK

Objective:To studythebiocompatibilityof CFR/PEEKcompositeinbonetissueafterimplantedinlumbar intervertebralspaceandto evaluateitsroleintheinterbodyfusioncomparedwithallograftbone.Meth ods:Thirteen beagleswerechosenamongwhich7animalswereimplantedwiththedisk-likeCFR/PEEKcompositein thelumbar intervertebralspaceand6wereimplantedwithallograftbone.X-ray,QCTandhistologicalexaminationwereem-ployedat6,12,and24monthspostoperatively.Results:TheX-rayresultswereinconformitywiththatof QCT.His-tologicalexaminationshowedtheanteriorsofttissueof theimplantexhibiteda nonspecificforeignbodyreaction withconnectivetissuethebiomaterials.Carbonfragmentwereseeninthesurroundingtissueandsomeof thedebric werephagocytosedby foreignbodygiantcell.Histologicalexaminationof boneand materialshowednew bone growthalonetheholeof CFR/PEEKimplant.Con clu sion:CFR/PEEKhasexcellentbiocompatibilityto bonetissue.

Ultrastructure of spermatozoa of Bullacta exarata （philippi） and its significance on reproductive evolution and physio-ecological adaptation

The morphology and ultrastructure of Bullacta exarata spermatozoa observed by light and transmission electron microscopy are presented in this paper. The spermatozoon is composed of head with a simple acrosomal complex and an elongated nucleus, and tail with a midpiece, principal piece and an end piece. The midpiece consists of a mitochondrial ring, and the principal piece is composed of axoneme and lateral fin. The structure of 5. exarata spermatozoa differs significantly from that of other gastropods, especially in the lateral fin and the principal piece, which was described scarcely before. A comparison is made between B. exarata and other gastropods, and its significance on reproductive evolution and physio-ecological adaptation is preliminarily discussed.