We have been done the detailed deletion mapping of chromosome (chr.) 8p21.3- 23 to localize the candidate tumor suppressor gene(s) (TSGs) loci as well as studied the mechanism of activation of c myc gene, located at c...We have been done the detailed deletion mapping of chromosome (chr.) 8p21.3- 23 to localize the candidate tumor suppressor gene(s) (TSGs) loci as well as studied the mechanism of activation of c myc gene, located at chr.8q24.1, by analyzing the amplification/rearrangement/HPV integration within approximately 580 kb of c myc locus in uterine cervical carcinoma (CaCx) of Indian patients. The association between the deletions in chr.8p21.3- 23 and alterations in the c myc locus has also been analyzed. The deletion mapping of chr.8p21.3- 23 was done by 15 microsatellite markers and the alterations in the c myc locus were analyzed by Southern hybridization using the pal- 1/c myc/mlvi- 4/HPV 16/18 probes in seven cervical intraepithelial neoplasia (CIN) and 55 primary uterine cervical carcinoma. The alterations in chr.8p/q have been correlated with the different clinicopathological parameters. Three discrete minimal deleted regions with high frequencies of loss of heterozygosity (LOH) (37- 43% ) were identified in the chr.8p23.1- 23.2 (D1), 8p23.1 (D2), and 8p 21.3- 22 (D3) regions within 0.41- 4.62 Mb. The deletion in the D1 region was significantly associated with the deletion in the D2 region (P = 0.03), whereas the deletion in D2 was marginally associated with the deletion in the D3 region (P = 0.07). The alterations in the c myc locus were seen in 43% of the samples. About 35% of the samples showed coalterations in both arms of chr.8. No significant association was observed with the alterations in chr.8p/q as well as with the different clinicopathological parameters. The deletions in chr.8p21.3- 23 and the alterations in the c myc locus are independently associated with the development of CaCx. The D1- D3 regions in chr.8p21.3- 23 could harbor candidate TSGs associated with the development of this tumor. The c myc gene was activated by amplification/rearrangement at the pal- 1/c myc/mlvi- 4 loci as well as HPV integration in the pal- 1 locus in this tumor.展开更多
我们利用特定位点扩增片段测序技术(specific-length amplified fragment sequencing,SLAF-seq)对籼稻窄叶青8(ZYQ8)和粳稻京系17(JX17)构建的双单倍体(doubled haploid,DH)群体进行了简化基因组测序。将DH群体测序数据与水稻参考基因...我们利用特定位点扩增片段测序技术(specific-length amplified fragment sequencing,SLAF-seq)对籼稻窄叶青8(ZYQ8)和粳稻京系17(JX17)构建的双单倍体(doubled haploid,DH)群体进行了简化基因组测序。将DH群体测序数据与水稻参考基因组进行比较分析,获得了70 997个在两个亲本中存在多态性的SLAF标签。通过进一步的筛选,我们利用8 940个高质量的SLAF标签构建了该群体的遗传图谱。图谱覆盖12个连锁群,总图距为2 294 71 c M,标记间的平均图距为0.26 c M。遗传图谱的连锁群与水稻物理图的相关系数统计显示,除了9号连锁群的相关系数为0.87外,其余的11个连锁群的相关系数均在0.95以上,表明该图谱的12个连锁群与水稻物理图有很好共线性关系。该高密度图谱的构建为我们进一步研究籼、粳差异基因提供了帮助。展开更多
对精细定位某一特定性状位点来说,高密度遗传图谱是一个非常实用的工具。本研究以水稻品种V20B/CPSLO17组合衍生的150份重组自交家系作为作图群体,利用特定位点扩增长度测序(SLAF-seq)技术,一种基于下一代测序技术进行大规模开发SNP和...对精细定位某一特定性状位点来说,高密度遗传图谱是一个非常实用的工具。本研究以水稻品种V20B/CPSLO17组合衍生的150份重组自交家系作为作图群体,利用特定位点扩增长度测序(SLAF-seq)技术,一种基于下一代测序技术进行大规模开发SNP和基因型分析的高通量新策略,开发SLAF标签和构建水稻高密度遗传图谱。我们共检测到67 017个高质量的SLAF标签,其中多态性SLAF有15 853个,符合构建高质量遗传图谱使用要求的多态性SLAF标签有8 657个。最终成功构建了一个包含12个连锁群,8 602个上图标记,总图距为2 508.65 c M,标记间平均距离为0.29 c M的高密度遗传图谱,该图谱可用于重要农艺性状QTL定位。展开更多
文摘We have been done the detailed deletion mapping of chromosome (chr.) 8p21.3- 23 to localize the candidate tumor suppressor gene(s) (TSGs) loci as well as studied the mechanism of activation of c myc gene, located at chr.8q24.1, by analyzing the amplification/rearrangement/HPV integration within approximately 580 kb of c myc locus in uterine cervical carcinoma (CaCx) of Indian patients. The association between the deletions in chr.8p21.3- 23 and alterations in the c myc locus has also been analyzed. The deletion mapping of chr.8p21.3- 23 was done by 15 microsatellite markers and the alterations in the c myc locus were analyzed by Southern hybridization using the pal- 1/c myc/mlvi- 4/HPV 16/18 probes in seven cervical intraepithelial neoplasia (CIN) and 55 primary uterine cervical carcinoma. The alterations in chr.8p/q have been correlated with the different clinicopathological parameters. Three discrete minimal deleted regions with high frequencies of loss of heterozygosity (LOH) (37- 43% ) were identified in the chr.8p23.1- 23.2 (D1), 8p23.1 (D2), and 8p 21.3- 22 (D3) regions within 0.41- 4.62 Mb. The deletion in the D1 region was significantly associated with the deletion in the D2 region (P = 0.03), whereas the deletion in D2 was marginally associated with the deletion in the D3 region (P = 0.07). The alterations in the c myc locus were seen in 43% of the samples. About 35% of the samples showed coalterations in both arms of chr.8. No significant association was observed with the alterations in chr.8p/q as well as with the different clinicopathological parameters. The deletions in chr.8p21.3- 23 and the alterations in the c myc locus are independently associated with the development of CaCx. The D1- D3 regions in chr.8p21.3- 23 could harbor candidate TSGs associated with the development of this tumor. The c myc gene was activated by amplification/rearrangement at the pal- 1/c myc/mlvi- 4 loci as well as HPV integration in the pal- 1 locus in this tumor.
文摘我们利用特定位点扩增片段测序技术(specific-length amplified fragment sequencing,SLAF-seq)对籼稻窄叶青8(ZYQ8)和粳稻京系17(JX17)构建的双单倍体(doubled haploid,DH)群体进行了简化基因组测序。将DH群体测序数据与水稻参考基因组进行比较分析,获得了70 997个在两个亲本中存在多态性的SLAF标签。通过进一步的筛选,我们利用8 940个高质量的SLAF标签构建了该群体的遗传图谱。图谱覆盖12个连锁群,总图距为2 294 71 c M,标记间的平均图距为0.26 c M。遗传图谱的连锁群与水稻物理图的相关系数统计显示,除了9号连锁群的相关系数为0.87外,其余的11个连锁群的相关系数均在0.95以上,表明该图谱的12个连锁群与水稻物理图有很好共线性关系。该高密度图谱的构建为我们进一步研究籼、粳差异基因提供了帮助。
文摘对精细定位某一特定性状位点来说,高密度遗传图谱是一个非常实用的工具。本研究以水稻品种V20B/CPSLO17组合衍生的150份重组自交家系作为作图群体,利用特定位点扩增长度测序(SLAF-seq)技术,一种基于下一代测序技术进行大规模开发SNP和基因型分析的高通量新策略,开发SLAF标签和构建水稻高密度遗传图谱。我们共检测到67 017个高质量的SLAF标签,其中多态性SLAF有15 853个,符合构建高质量遗传图谱使用要求的多态性SLAF标签有8 657个。最终成功构建了一个包含12个连锁群,8 602个上图标记,总图距为2 508.65 c M,标记间平均距离为0.29 c M的高密度遗传图谱,该图谱可用于重要农艺性状QTL定位。
