The FlaA gene from Vibrio harveyi marker, was cloned into the eukaryotic expression with a short nucleotide sequence encoding the Flag vector pcDNA3.1（＋） （designated as pcFlaA）. Ninety grouper （Epinephelus awoar...The FlaA gene from Vibrio harveyi marker, was cloned into the eukaryotic expression with a short nucleotide sequence encoding the Flag vector pcDNA3.1（＋） （designated as pcFlaA）. Ninety grouper （Epinephelus awoara） were separated into three equal size groups. An experimental group was immunized with pcFlaA, Control I group was immunized with the vector pcDNA3.1（＋）, and Control 1I group was immunized with PBS. The expression of pcFlaA mRNA and protein was examined using reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry. We also evaluated the immunogenicity and protective efficacy of pcFlaA against V. harveyi by measuring the lymphocyte proliferation response and serum levels of specific antibody and conducting a bacterial challenge test. We successfully transfected the fish muscle with pcFlaA. The pcFlaA mRNA and protein was expressed in the muscle cells for up to one month following injection. The proliferation response of lymphocytes in fish immunized with pcFlaA was significantly higher than in control group II. Furthermore, the immunized fish generated specific antibody. The vaccination also resulted in significantly higher survival during the bacterial challenge test.展开更多
Porous Fe3O4 sub-micro particles with sphere-like, cube-like and walnut-like morphologies were obtained by a two-step process, and the electromagnetic properties of the Fe3O4 particle/wax composites were investigated....Porous Fe3O4 sub-micro particles with sphere-like, cube-like and walnut-like morphologies were obtained by a two-step process, and the electromagnetic properties of the Fe3O4 particle/wax composites were investigated. The reflect loss was less than -20 dB for all of the composites in different frequency ranges. The cube-like and walnut-like Fe3O4 composites exhibit improved complex permittivity and permeability and dual-frequency and wide bandwidth absorption characteristics, which is mainly attributed to the larger shape anisotropy. Such a high absorption property indicates that these porous Fe3O4 particles with various morphologies are very promising for electromagnetic wave absorptive materials.展开更多
基金Supported by Fujian Science and Technology Innovation Foundation for Young Scientists (No.2006F3096)Scientific Research Foundation of Jimei University
文摘The FlaA gene from Vibrio harveyi marker, was cloned into the eukaryotic expression with a short nucleotide sequence encoding the Flag vector pcDNA3.1（＋） （designated as pcFlaA）. Ninety grouper （Epinephelus awoara） were separated into three equal size groups. An experimental group was immunized with pcFlaA, Control I group was immunized with the vector pcDNA3.1（＋）, and Control 1I group was immunized with PBS. The expression of pcFlaA mRNA and protein was examined using reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry. We also evaluated the immunogenicity and protective efficacy of pcFlaA against V. harveyi by measuring the lymphocyte proliferation response and serum levels of specific antibody and conducting a bacterial challenge test. We successfully transfected the fish muscle with pcFlaA. The pcFlaA mRNA and protein was expressed in the muscle cells for up to one month following injection. The proliferation response of lymphocytes in fish immunized with pcFlaA was significantly higher than in control group II. Furthermore, the immunized fish generated specific antibody. The vaccination also resulted in significantly higher survival during the bacterial challenge test.
基金supported by the National Natural Science Foundation of China (Grant Nos. 51072038, 50772025 and 21001035)NECT, Outstanding Youth Foundation of Heilongjiang Province (Grant No. JC201008)+4 种基金the Natural Science Foundation of Heilongjiang Province, China (Grant No. F200828)the Ministry of Science and Technology of China (Grant No. 2008DFR20420)the Fundamental Research Funds for the Central Universities (Grant Nos. HEUCFT1010, HEUCF101016, HEUCF20111124 and HEUCF101016)the National Basic Research Program of China (Grant No. 2007CB310500)Harbin Key Sci-Tech Project (Grant No. 2010AA4BG004)
文摘Porous Fe3O4 sub-micro particles with sphere-like, cube-like and walnut-like morphologies were obtained by a two-step process, and the electromagnetic properties of the Fe3O4 particle/wax composites were investigated. The reflect loss was less than -20 dB for all of the composites in different frequency ranges. The cube-like and walnut-like Fe3O4 composites exhibit improved complex permittivity and permeability and dual-frequency and wide bandwidth absorption characteristics, which is mainly attributed to the larger shape anisotropy. Such a high absorption property indicates that these porous Fe3O4 particles with various morphologies are very promising for electromagnetic wave absorptive materials.
