期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
IDENTIFICATION OF SPECIFIC PEPTIDE LIGANDS FOR B-LYMPHOMA CELL AND ITS EFFECT ON TYROSINE PHOSPHORYLATION AND CELL APOPTOSIS
1
作者 宋良文 马宪梅 +2 位作者 崔雪梅 李扬 王晓民 《Chinese Medical Sciences Journal》 CAS CSCD 2004年第1期44-50,共7页
Objective To search novel method for diagnosis and therapy of B-lymphoma, specific small mo-lecular peptide ligands against binding site of tumor cells were screened and its effects on signal transduction and cell apo... Objective To search novel method for diagnosis and therapy of B-lymphoma, specific small mo-lecular peptide ligands against binding site of tumor cells were screened and its effects on signal transduction and cell apoptosis were tested. Methods Specific peptide ligands were screened by binding with site of human B lymphoma cell(OC1LY8)using peptide-bead libraries. The identified peptides were characterized with responsible cellsby rebinding test. The role of tyrosine phosphorylation of peptide ligand was tested by Western blot; and its apoptosispromoting role was observed by confocal fluorescent microscope. Results Specific peptide ligand was able to bind specifically to site on cell surface and enter into cytoplasm. Tetrameric peptide ligand was able to strongly trigger signal transduction resulting in tyrosine phosphorylation and cellular apoptosis in OC1LY8 cell line. Conclusion Screened peptide ligand can effectively bind with OC1LY8 cell, stimulate cellular tyro-sine phosphorylation and induce cellular apoptosis. 展开更多
关键词 B-cell lymphoma peptide ligand APOPTOSIS
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部