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Toll样受体8及其免疫学功能研究进展 被引量:8
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作者 李立辉 郑世民 《免疫学杂志》 CAS CSCD 北大核心 2010年第10期913-915,共3页
Toll样受体8(toll-like receptor8,TLR8)不但能识别某些人工合成的具有抗病毒和抗肿瘤作用的小分子化合物,还可识别单链RNA病毒、鸟嘌呤核苷酸衍生物、特定合成的单链寡核苷酸(oligoribonucleotide,ORN)及双链小干扰RNA序列。活化TLR8... Toll样受体8(toll-like receptor8,TLR8)不但能识别某些人工合成的具有抗病毒和抗肿瘤作用的小分子化合物,还可识别单链RNA病毒、鸟嘌呤核苷酸衍生物、特定合成的单链寡核苷酸(oligoribonucleotide,ORN)及双链小干扰RNA序列。活化TLR8启动髓系分化因子88(myoloid differentiation factor 88,MyD88)依赖的信号通路,介导相应免疫应答。对TLR8及其免疫学功能深入系统的研究将对机体抗病毒感染和肿瘤治疗开辟新途径。本文对TLR8及其免疫学功能近年研究进展做了简要总结。 展开更多
关键词 Toll样受体8 分子结构 特异配体 信号转导 免疫学功能
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Fluorescent vital staining of plant sexual cell nuclei with DNA-specific fluorochromes and its application in gametoplast fusion 被引量:2
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作者 YANGHONGYUAN XINLIWU 《Cell Research》 SCIE CAS CSCD 1993年第2期121-130,共10页
DNA-binding fluorochromes are often used for vital staining of plant cell nuclei. However, it is not always sure whether the cells after staining still remain in living state. We chose several criteria to estimate the... DNA-binding fluorochromes are often used for vital staining of plant cell nuclei. However, it is not always sure whether the cells after staining still remain in living state. We chose several criteria to estimate the validity of real vital staining for sexual cell nuclei. These were: the cytoplasmic streaming in pollen tubes whose nuclei were stained, the simultaneous visualization of fluo-rochromatic reaction and nucleus staining in isolated generative cells, and the capability of isolated, prestained generative or sperm cells to fuse with other protoplasts. The results confirmed that 4',6-diamidino-2-phenylindole (DAPI), Hoechst 33258 and mithramycin could be used as real vital stains, though their efficiency varied from case to case; among them DAPI showed best effect. The fluorescent vital staining technique offered a useful means fori-dentification and selection of heterokaryons in gametoplast manipulation studies. 展开更多
关键词 fluorescent vital staining DNA-specific fluorochrome generative cell sperm cell gametoplast fusion.
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IDENTIFICATION OF SPECIFIC PEPTIDE LIGANDS FOR B-LYMPHOMA CELL AND ITS EFFECT ON TYROSINE PHOSPHORYLATION AND CELL APOPTOSIS
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作者 宋良文 马宪梅 +2 位作者 崔雪梅 李扬 王晓民 《Chinese Medical Sciences Journal》 CAS CSCD 2004年第1期44-50,共7页
Objective To search novel method for diagnosis and therapy of B-lymphoma, specific small mo-lecular peptide ligands against binding site of tumor cells were screened and its effects on signal transduction and cell apo... Objective To search novel method for diagnosis and therapy of B-lymphoma, specific small mo-lecular peptide ligands against binding site of tumor cells were screened and its effects on signal transduction and cell apoptosis were tested. Methods Specific peptide ligands were screened by binding with site of human B lymphoma cell(OC1LY8)using peptide-bead libraries. The identified peptides were characterized with responsible cellsby rebinding test. The role of tyrosine phosphorylation of peptide ligand was tested by Western blot; and its apoptosispromoting role was observed by confocal fluorescent microscope. Results Specific peptide ligand was able to bind specifically to site on cell surface and enter into cytoplasm. Tetrameric peptide ligand was able to strongly trigger signal transduction resulting in tyrosine phosphorylation and cellular apoptosis in OC1LY8 cell line. Conclusion Screened peptide ligand can effectively bind with OC1LY8 cell, stimulate cellular tyro-sine phosphorylation and induce cellular apoptosis. 展开更多
关键词 B-cell lymphoma peptide ligand APOPTOSIS
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