钩活术对早期2型糖尿病患者糖代谢水平的影响

目的:探究钩活术对早期2型糖尿病患者糖代谢的影响。方法:将80例早期2型糖尿病患者随机分为对照组与观察组,每组40例。对照组采用盐酸二甲双胍治疗,观察组采用钩活术治疗。治疗60 d后,比较两组患者临床疗效和治疗前后糖代谢指标[空腹血糖(FPG)、餐后2 h血糖(2 h PG)、糖化血红蛋白(HbA1c)],并评价安全性。结果:观察组总有效率为92.50%(37/40),高于对照组的75.00%(30/40),差异有统计学意义(P<0.05)。治疗后,两组患者FPG、2 h PG、HbA1c低于治疗前(P<0.05),且观察组低于对照组(P<0.05)。观察组1例患者出现伤口渗血,对照组1例患者出现不良反应,对症处理后症状消失。结论:钩活术治疗早期2型糖尿病效果显著,可改善患者糖代谢水平,且安全可靠。

Effect of electroacupuncture on electrogastrogram and gastric antrum ghrelin in rats with diabetic gastroparesis

Objective： To observe the effect of electroacupuncture（EA） on the electrogastrogram and gastric antrum ghrelin in rats with diabetic gastroparesis（DGP）.Methods： Fifty Sprague-Dawley（SD） rats were randomly divided into group A, group B, group C, group D and group E,10 rats in each group. Group A was the blank control group without intervention. Group B, Group C, Group D and Group E were treated with single dose intraperitoneal injection of 2% streptozotocin（STZ）, combined with 8-week high glucose and high fat diet to establish DGP rat models. Group B was the model group without treatment. Group C was the EA at acupoint group, was treated with EA at Zusanli（ST 36）, Liangmen（ST 21） and Sanyinjiao（SP 6）. Group D was the EA at non-acupoint group, was treated by EA at the control points of Zusanli（ST 36）, Liangmen（ST 21） and Sanyinjiao（SP 6）. Rats in the metoclopramide control group received 1.7% metoclopramide solution [10 mL/（kg·bw）] by gavage. Rat＇s blood glucose level was measured by blood glucose meter; gastric emptying rate was detected using phenol red as a marker; the electrogastrogram was detected by BL-420 F biological function system; the protein level of ghrelin was detected by enzyme-linked immunosorbent assay（ELISA）; the expression of ghrelin mRNA was detected by real-time polymerase chain reaction（RT-PCR）.Results： Compared with group A, the blood glucose of group B, C, D and E were significantly increased before and after the treatment（all P〈0.01）; after treatment, the gastric emptying rate of group B was significantly decreased（P〈0.01）,the migration rates of small intestine in group B, C, D and E were significantly lower（all P〈0.01）, and the protein content of ghrelin in group C was significantly decreased（P〈0.01）; the expressions of ghrelin mRNA were significantly increased in group B, C, D and E（all P〈0.01）, the mean amplitudes of electrogastrogram in group B and D were significantly decreased（both P〈0.01）. After treatment, compared with group B, the blood glucose of group C was significantly decreased（P〈0.05）, the gastric emptying rate and small intestine migration rate were significantly increased in group C and E（P〈0.05, P〈0.01）, the small intestinal migration rate was significantly increased in group D（P〈0.05）, the expression of ghrelin in protein and mRNA in group C was significantly lower（P〈0.01）, the expression of ghrelin mRNA in group E was significantly lower（P〈0.05）, and the mean amplitude of electrogastrogram in group C was significantly increased（P〈0.05）. After treatment, compared with group D, the protein and mRNA expressions of ghrelin in group C were significantly decreased（P〈0.01）. After treatment, compared with group E, the protein expression of ghrelin in group C was significantly decreased（P〈0.01）.Conclusion： EA at Zusanli（ST 36）, Liangmen（ST 21） and Sanyinjiao（SP 6） could regulate the blood glucose level of DGP model rats, enhance electrogastrogram activity, promote gastric emptying, and regulate ghrelin expression in protein and mRNA.

Study on metabolic intensity value changes of points on the face using thermal chromatography technology

Objective： To observe the changes of metabolic intensity value of points on the face before and after needling bilateral Hegu （LI 4） in healthy people and provide scientific basis for association between Hegu （LI 4） and face/mouth. Methods： A total of 45 healthy college students were selected in this study. Using medical thermography and Pennes bio-heat transfer model, the infrared thermograph images on the face before and after needling bilateral Hegu （LI 4） were collected to observe the distribution of metabolic intensity value on the face before acupuncture and changes in these values after needling bilateral Hegu （LI 4）. Results： Before acupuncture, Cuanzhu （BL 2） had the maximal metabolic intensity value. Its mean value was （0.71±0.23） W. Quanliao （SI 28） had the minimal metabolic intensity value. There were no left-right statistical significances in metabolic intensity values. After needling bilateral Hegu （LI 4）, the metabolic intensity values of most points on the face were increased. Kouheliao （LI 19） obtained the maximal increase： 0.35 W on average; and Yangbai （GB 24） obtained the minimal increase： 0.08 W on average. Conclusion： Points on both sides in healthy people have good symmetry in metabolic intensity value. After needling bilateral Hegu （LI 4）, the metabolic intensity values of points on the face were increased, especially points around the lips, which accords with the pathway of the Large Intestine Meridian on the head and face. This provided some scientific foundation for the association between Hegu （LI 4） and face/mouth.

Effect of acupuncture at points selected from different regions on SCF-kit signaling pathway in diabetic gastroparesis rats

Objective： To observe the effect of acupuncture at points selected from different regions on the positive expression of interstitial cells of Cajal （ICC） and the stem cell factor （SCF） in gastric antrum tissues in diabetic gastroparesis （DGP） rats, and to explore the influence of region-based point selection on the acupoint combination efficacy. Methods： Sixty Sprague-Dawley （SD） rats were randomly divided into a normal group （group A）, a model group （group B）, a group of Zusanli （ST 36） plus Zhongwan （CV 12） （group C）, a group of Zusanli （ST 36） plus Neiguan （PC 6） （group D）, and a group of Zusanli （ST 36） plus non-meridian non-acupoint points （group E）, based on the random number table （n=12）. DGP rat model was established by single intraperitoneal injection of 2% streptozotocin and common diet. After successful modeling, the rats were treated once a day for 4 weeks. Positive ICC and SCF expressions were measured by immunohistochemistry. Results： Compared with group A, the gastrointestinal propulsion rate of group B showed a statistically significant decrease （P〈0.05）. Compared with group B, the gastrointestinal propulsion rate and the expression of ICC in the gastric antrum were significantly higher in group C, group D and group E, and the between-group differences were statistically significant （P〈0.05）; the expression of C-kit protein in group C was statistically significantly higher than that in group D and group E （P〈0.05）. The expression of SCF protein was significantly increased in group C than in group B, and the difference was statistically significant （P〈0.05）. Conclusion： Acupuncture can improve the symptoms of delayed gastric emptying in DGP model rats, and regulate the expression of ICC and SCF in gastric antrum tissues. The effect of Zusanli （ST 36） plus Zhongwan （CV 12） in the gastric region is superior to that of the Zusanli （ST 36） plus distal Neiguan （PC 6） or non-meridian non-acupoint point, indicating that region-based point selection is the key factor affecting the effect of acupoint combination.