柱状田头菇(Agrocybe cylindracea)营养菌丝的生物学特性

对一种新兴食用菌——柱状田头菇(Agrocybe cylindracea)营养菌丝的生物学特性作了较为深入的研究,结果表明:菌丝最适生长温度为25～30℃,最适 pH 为5.0左右。在供试的11种碳源中,以甘露醇为最适碳源,其次是半乳糖、葡萄糖,而对纤维二糖、羧甲基纤维素钠、甘油的利用较差。对7种氮源的利用,以蛋白胨、酵母膏明显优于其它小分子含氮物质;在无氮培养基上不能生长。硫胺素(200u/L)、肌醇(10mg/L)对菌丝生长有明显促进作用。Fe^(2+)、Cu^(2+)、Zn^(2+)对菌丝生长有一定的促进作用,Mn^(2+)、Co^(2+)、Mo^(6+)无明显效果。红霉素(1 000 u/L)、氯霉素(1000u/L)、放线菌素 D(300μg/L)对柱状田头菇菌丝生长有极显著的抑制作用,培养基中加入500μg/L制霉菌素可完全抑制其生长。

杂交粳稻宁67A/K1722的特征特性及高产栽培技术探讨

杂交晚粳稻新组合宁67A/K1722由宁波市农科所育成,1994年配组,1998年引入我县,1999年开始大面积推广。2000年该品种通过浙江省农作物品种审定委员会审定。经我县两年种植,一致认为该组合高产、稳产、抗性好、优质,单双季兼用,并列入主推品种之一。

Physiological Basis of Photosynthetic Tolerance to Photooxidation and Shading in Rice

Thirty genotypes from rice germplasm were identified under photooxidation and shading condition and divided into four basic types : (1) cultivars tolerant to both photooxidation and shading, (2) cultivars tolerant to photooxidation but sensitive to shading, (3) cultivars tolerant to shading but sensitive to photooxidation, and (4) cultivars sensitive to photooxidation and shading. A comparative study of photosynthetic characteristics of a cultivar (cv. Wuyujing 3) that is tolerant and a cultivar (cv. Xiangxian) that is sensitive to both photoinhibition and shading showed that the photochemical efficiency of PSⅡ ( F v/F m ) and the content of PSⅡ_D1 protein in the tolerant cultivar “Wuyujing 3” decreased less under photoinhibition conditions as compared with “Xiangxian”. Under photooxidation conditions, superoxide dismutase was induced rapidly to a higher level and the active oxygen O - built up to a lower level in “Wuyujing 3” than in “Xiangxian”. At the same time, the photosynthetic rate decreased by 23% in “Wuyujing 3” vs. 64% in “Xiangxian”. Shading (20% natural light) during the booting stage caused only small decreases (7%-13%) in RuBisCO activity and the photosynthetic rate in “Wuyujing 3” but showed marked decreases (57%-64%) in “Xiangxian” which corresponded to the decreases in grain yield in the two cultivars (38% and 73%, respectively). The correlation analysis showed that the tolerance to photooxidation is mainly related to PSⅡ_D1 and that to shading is mainly related to RuBisCO activity. This study provided a simple and effective screening method and physiological basis for crop breeding in enhancing tolerance to both high and low radiation.

Responses of the seedlings of five dominant tree species in Changbai Mountain to soil water stress

Soil water stress was studied on the potted seedlings of five dominant tree species (Pinus koraienes Sieb.et Zucc., Fraxinus mandshurica Rupr., Juglans mandshurica Maxim, Tilia amurensis Rupr. and Quercus mongolica Fisch.ex Turcz) from the broadleaved/Korean pine forest in Changbai Mountain. Leaf growth, water transpiration and photosynthesis were compared for each species under three soil moisture conditions: 85%-100% (high water, CK), 65%-85% (Medium water, MW) and 45%-65% (low water, LW) of 37.4% water-holding capacity in field. The results showed that the characteristic of typical drought-resistance of the leaves is significantly developed. The net photosynthetic rate and water use efficiency of Fraxinus mandshurica were higher in MW than those in CK. But for the other four species, the net photosynthetic rate and water use efficiency in CK were lower than those in MW and LW. The transpiration rate responding to soil moistures varied from species to species.

Effect of High Temperature on Outcrossing Characteristics at the Fertility Sensitive Period of Photo-thermo Sensitive Genic Male Sterile (PTGMS) Rice Lines

Four sterile lines （Peiai64S, Y58S, Guangzhan 63-2S and H638S） and the restoring line R527 were used as materials. Five temperature gradients （24, 27, 30, 33 and 36 ℃ in artificial climate chamber） and the natural temperature （as control） were treated to the four sterile lines for 6 d in the fertility sensitive period of heading to flowering stage, respectively, to study the effects of temperature on physiological biochemical characteristics of young panicles and outcrossing characteristics. The results showed that the percentages of exerted stigma of Peiai 64S and Y58S were the highest at 27 ℃, which were 86.81% and 86.06%, respectively, while the percentages of exerted stigma of Guangzhan 63-2S and H638S were the highest at 24 ℃, which were 76.24% and 81.76%, respectively; the stigma viability of Peiai 64S, Y58S, Guangzhan 63-2S and H638S were the best at 24 ℃, which were 1.96, 2.12, 1.74 and 1.94, respectively; the outcrossing rates of Peiai 64S, Guangzhan 63-2S and H638S were the highest at 24 ℃, which were 58.87%, 54.22% and 50.50%, respectively, while the outcrossing rate of Y58S was the highest at 27 ℃, which was 58.96%; and the contents of peroxidase （POD） of the four sterile lines increased significantly at 33 ℃ compared with the control, while the contents of malondialdehyde （MDA） and proline increased significantly at 36 ℃ compared with the control. There were differences in temperature sensitivity between the male sterile lines, and the 24 ℃ treatment during the sensitive period was the best for the fertility sensitive period of Peiai 64S, while 27 ℃ was the best temperature for Y58S, Gangzhan 63-2S and H638S.

Drought Resistance of Slope Pioneer Plant Magnolia multiflora

[Objective] This study aimed to reveal responses of Magnolia multiflora to soil drought stress. [Method] Pot experiment was employed to simulate drought stress, and photosynthetic and physiological indices of M. multiflora were determined. [Result] The net photosynthetic rate （Pn） of M. multiflora did not change significantly under mild drought stress, and reduced significantly under moderate and severe drought stress. Drought stress reduced stomatal conductance （Gs）, transpiration rate （Tr） and intercellular CO2 concentration （Ci）, and compared with those in the control group, the Pn, Gs, Tr and Ci under severe drought stress declined by 61.04%, 86.27%, 87.77% and 42.63%, respectively. The malondialdehyde （MDA） content in M. multiflora leaves did not increase significantly under mild drought stress, and increased significantly under moderate and severe drought stress. The MDA content in M. multiflora leaves under severe drought stress was 1.63 times as high as that in the control group. The proline （Pro） and soluble sugar contents of M. multiflora increased significantly with the aggravation of drought stress, and those under severe drought stress were 8.06 times and 3.16 times respectively higher than those in the control group. [Conclusion] M. multiflora has a strong drought tolerance, and is suitable for growing in relatively arid environment. It can be used as candidate for vegetation restoration in hydropower engineering slope.

Using CropSyst to Simulate Spring Wheat Growth in Black Soil Zone of Northeast China

Available water and fertilizer have been the main limiting factors for yields of spring wheat, which occupies a large area of the black soil zone in northeast China; thus, the need to set up appropriate models for scenario analysis of cropping system models has been increasing. The capability of CropSyst, a cropping system simulation model, to simulate spring wheat growth of a widely grown spring cultivar, 'Longmai 19', in the black soil zone in northeast China under different water and nitrogen regimes was evaluated. Field data collected from a rotation experiment of three growing seasons (1992-1994) were used to calibrate and validate the model. The model was run for 3 years by providing initial conditions at the beginning of the rotation without reinitializing the model in later years in the rotation sequence. Crop input parameters were set based on measured data or taken from CropSyst manual. A few cultivar-specific parameters were adjusted within a reasonable range of fluctuation. The results demonstrated the robustness of CropSyst for simulating evapotranspiration, aboveground biomass, and grain yield of 'Longmai 19' spring wheat with the root mean square errors being 7%, 13% and 13% of the observed means for evapotranspiration (ET), grain yield and aboveground biomass, respectively. Although CropSyst was able to simulate spring production reasonably well, further evaluation and improvement of the model with a more detailed field database was desirable for agricultural systems in northeast China.

Biochemical characteristics of neonatal cholestasis induced by citrin deficiency

AIM:To explore differences in biochemical indices between neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD) and that with other etiologies. METHODS:Patients under 6 mo of age who were referred for investigation of conjugated hyperbiliru-binaemia from June 2003 to December 2010 were eligible for this study. After excluding diseases affecting the extrahepatic biliary system, all patients were screened for the two most common SLC25A13 mutations; the coding exons of the entire SLC25A13 gene was sequenced and Western blotting of citrin protein performed in selected cases. Patients in whom homo-zygous or compound heterozygous SLC25A13 mutation and/or absence of normal citrin protein was detected were defined as having NICCD. Cases in which no specific etiological factor could be ascertained after a com-prehensive conjugated hyperbilirubinaemia work-up were defined as idiopathic neonatal cholestasis (INC). Thirty-two NICCD patients, 250 INC patients, and 39 infants with cholangiography-confirmed biliary atresia (BA) were enrolled. Laboratory values at their first visit were abstracted from medical files and compared. RESULTS:Compared with BA and INC patients, the NICCD patients had significantly higher levels of total bile acid (TBA) [all measures are expressed as median (inter-quartile range):178.0 (111.2-236.4) μmol/L in NICCD vs 112.0 (84.9-153.9) μmol/L in BA and 103.0 (70.9-135.3) μmol/L in INC, P = 0.0001]. The NICCD patients had significantly lower direct bilirubin [D-Bil 59.6 (43.1-90.9) μmol/L in NICCD vs 134.0 (115.9-151.2) μmol/L in BA and 87.3 (63.0-123.6) μmol/L in INC, P = 0.0001]; alanine aminotransferase [ALT 34.0 (23.0-55.0) U/L in NICCD vs 108.0 (62.0-199.0) U/L in BA and 84.5 (46.0-166.0) U/L in INC, P = 0.0001]; aspartate aminotransferase [AST 74.0 (53.5-150.0) U/L in NICCD vs 153.0 (115.0-239.0) U/L in BA and 130.5 (81.0-223.0) U/L in INC, P = 0.0006]; albumin [34.9 (30.7-38.2) g/L in NICCD vs 38.4 (36.3-42.2) g/L in BA and 39.9 (37.0-42.3) g/L in INC, P = 0.0001]; glucose [3.2 (2.0-4.4) mmol/L in NICCD vs 4.1 (3.4-5.1) mmol/L in BA and 4.0 (3.4-4.6) mmol/L in INC, P = 0.0014] and total cholesterol [TCH 3.33 (2.97-4.00) mmol/L in N ICCD vs 4.57 (3.81-5.26) mmol/L in BA and 4.00 (3.24-4.74) mmol/L in INC, P = 0.0155] levels. The D-Bil to total bilirubin (T-Bil) ratio was significantly lower in NICCD patients [all measures are expressed as median (inter-quartile range):0.54 (0.40-0.74)] than that in BA patients [0.77 (0.72-0.81), P = 0.001] and that in INC patients [0.74 (0.59-0.80), P = 0.0045]. A much higher AST/ALT ratio was found in NICCD patients [2.46 (1.95-3.63)] compared to BA patients [1.38 (0.94-1.97), P = 0.0001] and INC patients [1.48 (1.10-2.26), P = 0.0001]. NICCD patients had significantly higher TBA/D-Bil ratio [3.36 (1.98-4.43) vs 0.85 (0.72-1.09) in BA patients and 1.04 (0.92-1.14) in INC patients, P = 0.0001], and TBA/TCH ratio [60.7 (32.4-70.9) vs 24.7 (19.8-30.2) in BA patients and 24.2 (21.4-26.9) in INC patients, P = 0.0001] compared to the BA and INC groups. CONCLUSION:NICCD has significantly different bio- chemical indices from BA or INC. TBA excretion in NICCD appeared to be more severely disturbed than that of bilirubin and cholesterol.

Cymolomia hartigiana (Saxesen,1840)(Lepidoptera: Tortricidae) at-tacking to Abies nephrolepis Max. New to Daxing'anling,Heilongji-ang,China

An Olethreutine speciesCymolomia hartigiana (Saxesen, 1840), attacking toAbies nephrolepis Max., is reported for the first time from Daxing'anling, Heilongjiang, China. The morphological characteristics are briefly re-described with illustrations. Also its damage and biology are noted. Key words Systematics - Lepidoptera - Tortricidae - Cymolomia - Daxing'anling - China CLC number Q969.429.2 - S763.3 Document code A Foundation item: This study was carried out under the financial support by KOSEF (Korea Science & Engineering Foundation) with the program of “Korea and China Young Scientist Exchange Program” (2002–2003).Biography: *BYUN Bong-Kya (1963-), male, Ph.D., Researcher in Korea National Arboretum, Korea.Responsible editor: Chai Ruihai

Establishment of a transplantation tumor model of human osteosarcoma in chick embryo

Objective: We established a transplantation tumor model of human osteosarcoma in chick embryo, studied its morphological and biological characteristics, and observed its dynamic process of angiogenesis induction so that a simple and practical model can be provided for studying osteosarcoma. Methods: Human osteosarcoma cells at different concentrations were inoculated in chorioallantoic membrane (CAM) of chick embryos at different embryonic ages to observe the factors affecting the survival of the transplanted osteosarcoma in chick embryo, growth characteristics of the transplantation tumor, and the morphological characteristics and biological characteristics of the transplantation tumor. Results: The transplantation tumor model of human osteosarcoma in chick embryo was successfully established. It was found that the transplantation tumor was easy to grow and it showed strong angiogenesis-inducing effects. Under the light microscope, the transplantation tumor showed a similar tissue structure to human osteosarcoma. Conclusion: It is feasible to establish a transplantation tumor model of human osteosarcoma in chick embryo. The model can be easily duplicated with a simple operation, which provides a useful animal model for studying osteosarcoma.

湘北黄瓜品种比较试验

为筛选出适合在湘北地区栽种的露地黄瓜品种,以引进的中研21、津优21号、天佳9号、津耘401等4个品种为试验材料进行品种比较试验。试验结果表明,天佳9号黄瓜商品性好、抗病性强、产量高、生长势强、耐高温,平均单果重为303.30 g,亩产量为7045.52 kg,比对照亩产量增加1314.66 kg,差异达到显著水平,有很好的推广应用前景,适宜在湘北地区大力推广。

Replacement of Dietary Fish Oil with Vegetable Oils Improves the Growth and Flesh Quality of Large Yellow Croaker(Larmichthys crocea)

We investigated the effect of the replacement of dietary fish oil with vegetable oils on the growth and flesh quality of large yellow croaker(Larmichthys crocea). The basal diet(FO) was formulated to contain 66.5% fish meal and 6.4% menhaden fish oil; whereas the other 3 experimental diets were formulated by replacing the fish oil with 50% soybean oil(SO50), 100% soybean oil(SO100) and 100% palm oil(PO100), respectively. The 4 diets were randomly assigned to 4 floating sea cages(3.0 m × 3.0 m × 3.0 m), and each was stocked with 250 fish individuals with an initial average weight of 245.29 g ± 7.45 g. The fish were fed to apparent satiation twice a day at 5:00 and 17:00, respectively, for 12 weeks. Experimental analysis showed that the specific growth rate of fish fed SO50 or PO100 were significantly higher than that of fish fed FO or SO100(P<0.05), and crude lipid contents of ventral muscle and viscera were significantly lower in fish fed FO than in those fed the other 3 diets(P<0.05). No significant differences in condition factor, viscerosomatic index, hepatosomatic index, gutted yield and colorimetric values of fish among the dietary treatments were observed(P>0.05). Compared to FO diet, SO50, SO100 and PO100 diets led to substantial decreases in the liquid loss and water loss from fresh fillets(1 d, 4℃)(P<0.05). Similarly, thiobarbituric acid reactive substance(TBARS) values of fillets under different storage conditions(1 d, 4℃; 7 d, 4℃; 4 weeks,-20℃; 8 weeks,-20℃) decreased significantly after partial or complete replacement of fish oil with vegetable oils. These findings indicated that the growth performance and selected flesh quality properties(liquid holding capacity and TBARS value) of large yellow croaker were substantially improved by replacing dietary fish oil with vegetable oils.

Invasion and spreading of Cabomba caroliniana revealed by RAPD markers

Applying randomly amplified polymorphic DNA (RAPD), the genetic variation of Cabomba caro- liniana Gray (cabomba or fanwort), a new alien plant in China, was analyzed in this paper. Total 143 bands, in- cluding 47 polymorphic bands, were amplified from 23 primers in 20 samples. The sampling distance was large, but its genetic diversity was low. The main results were that: (1) Cabomba, which grew and dispersed mainly in fragment, was an abundant and dominant species in freshwater, and its main dispersal mechanism was vegetative reproduction. (2) Cabomba was originally introduced into China as an aquarium submerged plant. Somehow, those discarded cabomba became invasive species in the areas of Hangzhou, Shanghai, and Meicheng, and other places. (3) Although the level of genetic diversity in cabomba was low, their rapid dispersion and propagation could seriously harm to local aquatic community. Therefore, specific measure should be used to control cabomba from uncontrolled spreading and damage to local vegetation communities.

A Novel Method for Determining Microbial Kinetics

Understanding microbial growth is essential to any research conducted in the fields of microbiology and biotechnology. Current methods of determining growth characteristics of microbes involve subjective graphical interpretations of linearized logarithmic data. Reducing error in logistical data decreases disparity between graphical and analytical predictions of microbial characteristics. In this study, a method has been developed to calculate the kinetics of microbial characteristics utilizing a modified Maclaurin series. Convergence of this series approaches the true kinetic value of microbial characteristics to include specific growth rates. In this research, a modified Maclaurin series is used to evaluate microbial kinetics in comparison to graphical determinations.

Interactions between Soil Characteristics, Environmental Factors, and Plant Species Abundance: A Case Study in the Karst Mountains of Longhushan Nature Reserve, Southwest China

The ability to manage and restore plant communities in the face of human-induced landscape change may rely on our ability to predict how species respond to environmental variables.Understanding this response requires examining factors or their interactions that have influence on plant and resource availability.Our objective was to analyze the relationships between changes in plant abundance and the interaction among environmental habitat factors including soil, geological(rock type), and other environmental variables in the Longhushan karst mountains ecosystem.Species density and dominance were examined using ANOVA, ANCOVA,and Generalized Linear Models to establish the single or combined effects of these groups of factors.The results showed that trends in abundance were mainly affected by rock type(related to the percentage content of dolomite and calcite), soil characteristics in association with topography.Both plant indices were higher in dolomite dominated areas and varied positively with moisture, and elevation, but negatively with organic matter, while density also increased with slope degree.The results demonstrate that significant variations in species abundance was produced with the combination of variables from soil, geological, andenvironmental factors, suggesting their interaction influence on plants.We postulate that spatial variations in plant abundance in karst ecosystem depends on the carbonate rock type in addition to water and nutrient availability which are mainly controlled by topography and other factors such as soil texture and temperature.The study suggests that in karst areas carbonate rock type, in addition to local environmental variables, should be taken into account when analyzing the factors that have impact on plant communities.

Comparison of Biochemical Properties of Recombinant and Native Phanerochaete flavido-alba Laccases

Laccases are blue copper oxidases （E.C. 1.10.3.2 benzenediol：oxygen oxidoreductase） that catalyze the one-electron oxidation of phenolics, aromatic amines and other electron-rich substrates with the concomitant reduction of 02 to H20. They are currently seen as highly interesting industrial enzymes because of their broad substrate specificity. The Phanerochaete flavido-alba laccase is expressed and secreted as a soluble active enzyme by Aspergillus niger （rLac-LPFA）. rLac-LPFA is easily purified to homogeneity. Metal ions like HgCI2, KC12, FeSO4 and MgSO4 at a concentration of 2 mM have inhibiting effect on recombinant and native laccase, whereas, CuSO4 and MnSO4 moderately increase both enzyme activities. Two potential inhibitors （sodium azide and EDTA） inhibited enzyme activity, whereas, urea and SDS have no effect on enzyme activity. The Km and V,,ax values for recombinant laccase are 0.65 mM and 300 U/mg respectively for 2,6-DMP as substrate.

Effects of the hummock-depression microhabitat on plant communities of alpine marshy meadows in the Yellow River Source Zone,China

Our objectives are to examine the effects of hummock-depression spatial heterogeneity on plant communities and soil properties,and to understand the process of maintaining and adjusting microtopography-mediated hydrological inputs and their spatial fluctuations that produce obvious microhabitats.We set up 36 plots(1 m×1 m)and sampled 45 plant and 225 soil samples in flooded(FH)and non-flooded hummocks(NFH)and depressions of the marshy,and the surrounding non-wetland meadows as well as in the Yellow River Source Zone,west China.We evaluated whether the alpine marshy wetland has a fertile island effect by the comparison method.Our results show that hummock presence can increase the spatial heterogeneity of the microhabitat and promote the plant diversity and soil fertility of the Kobresia tibetica community.Plant height,coverage,above-ground biomass,species richness and diversity were significantly higher in the FH and NFH microhabitat than in the areas between hummocks and surrounding non-wetland meadows.Compared with broad alpine meadows,the hummock-depression complex provided a microhabitat favorable to the growth of Cyperaceae.In the 0-50 cm soil layer,the closer the soil layer was to the ground surface,the higher its soil organic carbon and total nitrogen contents.Thus,in deeper layers,the gap between soil nutrients in wetland hummock-depression microhabitat and in the surrounding alpine meadows becomes smaller.Hence,the wetland hummock-depression microhabitat formed a fertile island pattern.Therefore,these results contribute toward improving our understanding of ecosystem restoration in alpine marshy meadows.

Seasonal Effect of Geomorphological Chronosequence Features on Soil Biota Dynamics

Numerous studies have been devoted to the physical-chemical weathering processes leading to the creation of unique soil formations having their own history that induce soil-biotic diversity.However,the extent to which unique geomorphic formations influence soil biotic seasonal variation is not clear.Our aim was to define seasonal variations of soil biota in soils of different-aged terraces of the Makhtesh Ramon anticline erosional cirque in southern Israel.The strong effect of Makhtesh Ramon (Ramon crater) erosional fluvial terrace age initiated by climatic changes during the Late Pleistocene- Early Holocene period on seasonal variations in both soil properties and the abundance and composition of soil biota were demonstrated.However,age dependence was not constant and values for observed soil properties and microbial activity were negligible between younger and older terraces for certain seasons,while free-living nematodes along with bacterial-feeding group were strongly dependent on the geomorphic features of the ages throughout the study period.