采用中药白及作栓塞剂,对5只正常犬肺作经皮肺直接注射。从3个阶段进行 X 线-病理对照观察。结果表明:局部肺组织的病理演变大致可概括为以肺段分布的肺泡内渗出和间质内急性血管床闭塞,局限性坏死和以异物巨细胞增生为主的修复反应3个...采用中药白及作栓塞剂,对5只正常犬肺作经皮肺直接注射。从3个阶段进行 X 线-病理对照观察。结果表明:局部肺组织的病理演变大致可概括为以肺段分布的肺泡内渗出和间质内急性血管床闭塞,局限性坏死和以异物巨细胞增生为主的修复反应3个阶段,在穿刺灶外3cm 的肺组织追踪观察,基本上为正常 X 线组织学象。在犬肺铸型模型上,观察到被栓塞剂封闭的部位形成一个类圆形的缺损灶;周围肺动静脉和细支气管残端呈截断样闭塞,与 X 线肺片上穿刺造影部位相一致。展开更多
OBJECTIVE: To investigate the effect of Chaiqinchengqi decoction(CQCQD) on inositol requiring enzyme 1α(IRE1α) in alveolar macrophages(AMs)of the dog model of acute necrotising pancreatitis(ANP) induced by sodium ta...OBJECTIVE: To investigate the effect of Chaiqinchengqi decoction(CQCQD) on inositol requiring enzyme 1α(IRE1α) in alveolar macrophages(AMs)of the dog model of acute necrotising pancreatitis(ANP) induced by sodium taurocholate.METHODS: Fifteen beagle dogs were randomised into a control group,ANP group and CQCQD group(n = 5 per group). ANP was induced by a retrograde duct injection of 50 mg/kg of 5% sodium taurocholate. The dogs in the control group received injections of the same volume of saline as the sodium taurocholate. After the models were induced,the dogs in the CQCQD group were administered 10 m L/kg CQCQD every 2 h for 6 h. Two hours after the last administration of either CQCQD or saline,they were sacrificed by anaesthesia. AMs were collected to determine the IRE1α and Interleukin-1β(IL-1β)m RNA and protein expression,and pancreatic tissues were collected for histopathology analysis.RESULTS: Compared with the ANP group,the m RNA and protein expression of IRE1α and the protein expression of IL-1β of AMs in the CQCQD group were significantly down-regulated,and the pancreatic histopathology score of the CQCQD group also was lower. There was no significant difference in the m RNA expression of IL-1β of AMs between the two groups.CONCLUSION: The CQCQD-induced down-regulation of the IL-1β protein expression may involve the down-regulation of the m RNA and protein expression of IRE1α in AMs.展开更多
文摘采用中药白及作栓塞剂,对5只正常犬肺作经皮肺直接注射。从3个阶段进行 X 线-病理对照观察。结果表明:局部肺组织的病理演变大致可概括为以肺段分布的肺泡内渗出和间质内急性血管床闭塞,局限性坏死和以异物巨细胞增生为主的修复反应3个阶段,在穿刺灶外3cm 的肺组织追踪观察,基本上为正常 X 线组织学象。在犬肺铸型模型上,观察到被栓塞剂封闭的部位形成一个类圆形的缺损灶;周围肺动静脉和细支气管残端呈截断样闭塞,与 X 线肺片上穿刺造影部位相一致。
基金Supported by the National Natural Science Foundation of China(the Mechanism of Protein Molecular Brake of Suppression the Releasing of Pro-Inflammatory Cytokines of Macrophage in Acute Necrotizing Pancreatitis by Chaiqinchengqi Decoction,No.81072910)Science and Technology Support Program of Sichuan(Effect of Chaiqincheng Decoction on the Gastrointestinal Dysfunction in Severe Acute Pancreatitis and the Establishment of Evaluation System,No.2014SZ0211)
文摘OBJECTIVE: To investigate the effect of Chaiqinchengqi decoction(CQCQD) on inositol requiring enzyme 1α(IRE1α) in alveolar macrophages(AMs)of the dog model of acute necrotising pancreatitis(ANP) induced by sodium taurocholate.METHODS: Fifteen beagle dogs were randomised into a control group,ANP group and CQCQD group(n = 5 per group). ANP was induced by a retrograde duct injection of 50 mg/kg of 5% sodium taurocholate. The dogs in the control group received injections of the same volume of saline as the sodium taurocholate. After the models were induced,the dogs in the CQCQD group were administered 10 m L/kg CQCQD every 2 h for 6 h. Two hours after the last administration of either CQCQD or saline,they were sacrificed by anaesthesia. AMs were collected to determine the IRE1α and Interleukin-1β(IL-1β)m RNA and protein expression,and pancreatic tissues were collected for histopathology analysis.RESULTS: Compared with the ANP group,the m RNA and protein expression of IRE1α and the protein expression of IL-1β of AMs in the CQCQD group were significantly down-regulated,and the pancreatic histopathology score of the CQCQD group also was lower. There was no significant difference in the m RNA expression of IL-1β of AMs between the two groups.CONCLUSION: The CQCQD-induced down-regulation of the IL-1β protein expression may involve the down-regulation of the m RNA and protein expression of IRE1α in AMs.
