In order to survey the infectious situation of canine coronavirus (CCV) in giant panda population, a virus neutralization test detecting specific antibodies against CCV in giant panda抯 sera was established by using t...In order to survey the infectious situation of canine coronavirus (CCV) in giant panda population, a virus neutralization test detecting specific antibodies against CCV in giant panda抯 sera was established by using two-fold dilutions of serum and 100 TCID50 of the virus. The 62 sera samples of giant pandas, which were gathered from zoos and reserve region of Sichuan Province, China were detected. The neutralization antibody titer of 1:4 was recognized as the positive criterion, 8 sera samples were detected to be positive, and the positive rate was 12.9%. The titers of neutralizing antibody ranged from 1:8 to 1:32. It was the first comprehensive investigation on neutralization antibodies against CCV in giant panda population in China. The results of study showed that the infection of CCV in giant panda population was universal, which has posed a threat to the health of giant panda. Therefore, it is incumbent on us to study safe and effective vaccines to protect giant panda against CCV infection.展开更多
为表达和获取具有抗凝血功能的犬钩虫抗凝血肽(A cAP c2),采用搭桥PCR方法合成犬钩虫抗凝血肽全长双链cDNA序列,A cAP c2 cDNA全序列连入表达载体pTW IN 1上构建成具有蛋白自剪切功能的表达载体pTW IN 1-A cAP c2,将阳性重组子转入表达...为表达和获取具有抗凝血功能的犬钩虫抗凝血肽(A cAP c2),采用搭桥PCR方法合成犬钩虫抗凝血肽全长双链cDNA序列,A cAP c2 cDNA全序列连入表达载体pTW IN 1上构建成具有蛋白自剪切功能的表达载体pTW IN 1-A cAP c2,将阳性重组子转入表达型大肠杆菌E.coli ER 2566进行表达。表达的融合蛋白A cAP c2-in te in2-CBD为可溶性蛋白,且融合蛋白约占菌体总蛋白的30.1%,经蛋白质印迹分析确定表达产物是具有CBD蛋白的特异性融合蛋白。融合蛋白A cAP c2-in te in2-CBD经几丁质柱高效亲和纯化,并经β-巯基乙醇诱导的独特的在柱自剪切后,得到目的蛋白A cAP c2,经SDS-PAGE分析在21 KD处呈现目的条带,所得的可溶性A cAP c2分子量符合其天然活性的二聚体形式。对A cAP c2表达和纯化工艺上的改进,方便了A cAP c2的获取,A cAP c2氨基酸序列的生物信息学分析初步阐明了A cAP c2的结构和功能的关系,为进一步研究A cAP c2的抗凝血机制及其作为抗凝血药的临床应用奠定了基础。展开更多
Electrolytic detachable coils (EDC) have been the main embolic materi als for intracranial aneurysms. Liquid aneurysmal embolic materials represented by cellulose acetate polymer (CAP) are still in controversy. In thi...Electrolytic detachable coils (EDC) have been the main embolic materi als for intracranial aneurysms. Liquid aneurysmal embolic materials represented by cellulose acetate polymer (CAP) are still in controversy. In this research, t he embolization results and pathological reactions after embolization of canine aneurysmal models with EDC or CAP were observed and compared. Methods. The canine aneurysmal models constructed by anastomosis of venous pouch es were randomly grouped. The aneurysms were respectively occluded with CAP and electrolytic detachable coils that was named by Wu electrolytic detachable coil (WEDC) and made by us. Angiogram follow ups were performed at 24 hour, 2 week , and 2 month after embolization. The occluded aneurysms were dissected in each stage for light microscopic, electron microscopic, and histochemical research. Results. The effect of embolization was significantly better with WEDC than that with CAP . Post embolized complications such as aneurysm rupture and stenosis of parent arteries could only be found in CAP group. Pathol ogical research showed that CAP mass could packed the aneurysms more densely tha n coils. Acute chemical damage of aneurysmal wall and inflammatory cell infiltra tion was prominently found in early stage after CAP embolization. Organization of thrombus inside aneurysms and formation of endothelial tissue over the orific es of aneurysmal necks could be found in both groups 2 months after embolization . But parts of coils might be exposed outside endothelial layer. Conclusions. EDC are still the most safe, efficient, and reliable instruments to embolize aneurysm. CAP should be improved further to solve the problem of stron g chemical corrosion and difficulty in control before it is widely used.展开更多
基金Supported by the Natural Scienceof Tianjin(19JCQNJC13700)the Research Project of Tianjin Education Commission(2018KJ185)the University-level Innovative and Entrepreneurial Training Plan for College Students(202110061035)。
基金This research was supported by National Science Founda-tion of China (No. 30000123) and Conversation Department of Wildlife Ani-mal & Plants of State Forestry Bureau.
文摘In order to survey the infectious situation of canine coronavirus (CCV) in giant panda population, a virus neutralization test detecting specific antibodies against CCV in giant panda抯 sera was established by using two-fold dilutions of serum and 100 TCID50 of the virus. The 62 sera samples of giant pandas, which were gathered from zoos and reserve region of Sichuan Province, China were detected. The neutralization antibody titer of 1:4 was recognized as the positive criterion, 8 sera samples were detected to be positive, and the positive rate was 12.9%. The titers of neutralizing antibody ranged from 1:8 to 1:32. It was the first comprehensive investigation on neutralization antibodies against CCV in giant panda population in China. The results of study showed that the infection of CCV in giant panda population was universal, which has posed a threat to the health of giant panda. Therefore, it is incumbent on us to study safe and effective vaccines to protect giant panda against CCV infection.
文摘为表达和获取具有抗凝血功能的犬钩虫抗凝血肽(A cAP c2),采用搭桥PCR方法合成犬钩虫抗凝血肽全长双链cDNA序列,A cAP c2 cDNA全序列连入表达载体pTW IN 1上构建成具有蛋白自剪切功能的表达载体pTW IN 1-A cAP c2,将阳性重组子转入表达型大肠杆菌E.coli ER 2566进行表达。表达的融合蛋白A cAP c2-in te in2-CBD为可溶性蛋白,且融合蛋白约占菌体总蛋白的30.1%,经蛋白质印迹分析确定表达产物是具有CBD蛋白的特异性融合蛋白。融合蛋白A cAP c2-in te in2-CBD经几丁质柱高效亲和纯化,并经β-巯基乙醇诱导的独特的在柱自剪切后,得到目的蛋白A cAP c2,经SDS-PAGE分析在21 KD处呈现目的条带,所得的可溶性A cAP c2分子量符合其天然活性的二聚体形式。对A cAP c2表达和纯化工艺上的改进,方便了A cAP c2的获取,A cAP c2氨基酸序列的生物信息学分析初步阐明了A cAP c2的结构和功能的关系,为进一步研究A cAP c2的抗凝血机制及其作为抗凝血药的临床应用奠定了基础。
文摘Electrolytic detachable coils (EDC) have been the main embolic materi als for intracranial aneurysms. Liquid aneurysmal embolic materials represented by cellulose acetate polymer (CAP) are still in controversy. In this research, t he embolization results and pathological reactions after embolization of canine aneurysmal models with EDC or CAP were observed and compared. Methods. The canine aneurysmal models constructed by anastomosis of venous pouch es were randomly grouped. The aneurysms were respectively occluded with CAP and electrolytic detachable coils that was named by Wu electrolytic detachable coil (WEDC) and made by us. Angiogram follow ups were performed at 24 hour, 2 week , and 2 month after embolization. The occluded aneurysms were dissected in each stage for light microscopic, electron microscopic, and histochemical research. Results. The effect of embolization was significantly better with WEDC than that with CAP . Post embolized complications such as aneurysm rupture and stenosis of parent arteries could only be found in CAP group. Pathol ogical research showed that CAP mass could packed the aneurysms more densely tha n coils. Acute chemical damage of aneurysmal wall and inflammatory cell infiltra tion was prominently found in early stage after CAP embolization. Organization of thrombus inside aneurysms and formation of endothelial tissue over the orific es of aneurysmal necks could be found in both groups 2 months after embolization . But parts of coils might be exposed outside endothelial layer. Conclusions. EDC are still the most safe, efficient, and reliable instruments to embolize aneurysm. CAP should be improved further to solve the problem of stron g chemical corrosion and difficulty in control before it is widely used.
