为研究冻藏时间对猪背最长肌蛋白质特性的影响,本文主要采用凝胶电泳、差示量热扫描技术分析了猪背最长肌在-18℃冻藏0 d和1、3、6、9、12、15月后,其蛋白质的疏水性、巯基含量、降解情况、热稳定性的变化。结果表明:随冻藏时间的延长,...为研究冻藏时间对猪背最长肌蛋白质特性的影响,本文主要采用凝胶电泳、差示量热扫描技术分析了猪背最长肌在-18℃冻藏0 d和1、3、6、9、12、15月后,其蛋白质的疏水性、巯基含量、降解情况、热稳定性的变化。结果表明:随冻藏时间的延长,蛋白质溶解度显著降低(p<0.05),肌原纤维蛋白(MPI)的表面疏水性和巯基含量升高,发生降解的MPI主要集中在67 k Da和20~29 k Da;肌球蛋白和肌浆蛋白的变性温度和焓值呈现逐渐降低的趋势,表明这两类蛋白的热稳定性降低。在冻藏的前3个月内,猪背最长肌的各项指标维持在较好的水平,从第6个月开始,所有指标的变化幅值增加,变化速率加快。研究发现,冻藏6个月以后,猪背最长肌蛋白质品质开始加速下降。展开更多
[ Objective] This study was to investigate the distribution of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa, so as to provide references for revealing the physiological functions of this gene. [Method] Wi...[ Objective] This study was to investigate the distribution of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa, so as to provide references for revealing the physiological functions of this gene. [Method] With the mRNA from different tissues as template, RT-PCR amplification was carried out for cloning cDNA of hybrid pig, which was then used for PCR reaction using specific primers; the amplification products were separated by gel electrophoresis and analyzed by sequencing for detecting the distribution pattern of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa. [ Result] H-FABP expressed in all the twelve tissues including subcutaneous fat, abdominal fat, mammary gland, Iongissimus dorsi muscle, dorsal deltoid muscle, heart, liver, spleen, lung, kidney, ileum and duodenum. [ Conclusion] The wide distribution of H-FABP gene suggests that its functions are important and multiple.展开更多
文摘为研究冻藏时间对猪背最长肌蛋白质特性的影响,本文主要采用凝胶电泳、差示量热扫描技术分析了猪背最长肌在-18℃冻藏0 d和1、3、6、9、12、15月后,其蛋白质的疏水性、巯基含量、降解情况、热稳定性的变化。结果表明:随冻藏时间的延长,蛋白质溶解度显著降低(p<0.05),肌原纤维蛋白(MPI)的表面疏水性和巯基含量升高,发生降解的MPI主要集中在67 k Da和20~29 k Da;肌球蛋白和肌浆蛋白的变性温度和焓值呈现逐渐降低的趋势,表明这两类蛋白的热稳定性降低。在冻藏的前3个月内,猪背最长肌的各项指标维持在较好的水平,从第6个月开始,所有指标的变化幅值增加,变化速率加快。研究发现,冻藏6个月以后,猪背最长肌蛋白质品质开始加速下降。
基金Supported by the National High Technology Research and Develop-ment Program of China(No.2008AA10Z136)the Program of Liaoning Provincial Science-Technology Department(20070567)~~
文摘[ Objective] This study was to investigate the distribution of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa, so as to provide references for revealing the physiological functions of this gene. [Method] With the mRNA from different tissues as template, RT-PCR amplification was carried out for cloning cDNA of hybrid pig, which was then used for PCR reaction using specific primers; the amplification products were separated by gel electrophoresis and analyzed by sequencing for detecting the distribution pattern of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa. [ Result] H-FABP expressed in all the twelve tissues including subcutaneous fat, abdominal fat, mammary gland, Iongissimus dorsi muscle, dorsal deltoid muscle, heart, liver, spleen, lung, kidney, ileum and duodenum. [ Conclusion] The wide distribution of H-FABP gene suggests that its functions are important and multiple.
