The crushing of leaves is the most time and effort-consuming part in DNA extraction which is a fundamental step in the study of molecular biology. In this study, a new rapid and batch-oriented crushing method for DNA ...The crushing of leaves is the most time and effort-consuming part in DNA extraction which is a fundamental step in the study of molecular biology. In this study, a new rapid and batch-oriented crushing method for DNA extraction from maize leaves was developed. In addition, the practicability of the developed method in molecular marker-assisted breeding was verified using SSR molecular maker technology so as to provide a rapid, batch-oriented, low-cost and non-toxic leafcrushing method for a large number of molecular marker tests, improving test efficiency.展开更多
Corn silage is the most popular and important source of energy and fber for ruminants. Much research focused on the chopping length, additives, sealing system, harmful bacteria and their toxins, and so forth, has been...Corn silage is the most popular and important source of energy and fber for ruminants. Much research focused on the chopping length, additives, sealing system, harmful bacteria and their toxins, and so forth, has been done on corn silage both at home in China and abroad. Some suggestions were presented in this review： （1） Corn silage modulation： Whole-plant corn harvested at the milk-ripe stage with a dry matter （DM） content of 30%~40% was selected as silage material and treated by rubbing and cutting. In the process of silage, commercial Lactobacillus buchneri was inoculated, exogenous protease added appropriately, cellulose enzyme added depending on the phenological period, the silage container and the species of the animals, and coextruded polyethylene？polyamide oxygen barrier flm （OB） selected as the seal material. （2） Strengthening management： Aerobic exposure should be avoided at the storage stage and reduced during the feeding phase. The proportion of corn silage was increased depending on the type of animal and their management. （3） Suggestions for further research： Further studies would be needed for the effects of liquid and solid wastes on soil, water and vegetation, and the treatment of these wastes.展开更多
基金Supported by Special Fund for Agro-scientific Research in the Public Interest of China(201303008)Science and Technology Plan Project of Guangdong Province(2012B020301006)Key Breeding Project for Special Maize of Department of Agriculture of Guangdong Province(B3071328)~~
文摘The crushing of leaves is the most time and effort-consuming part in DNA extraction which is a fundamental step in the study of molecular biology. In this study, a new rapid and batch-oriented crushing method for DNA extraction from maize leaves was developed. In addition, the practicability of the developed method in molecular marker-assisted breeding was verified using SSR molecular maker technology so as to provide a rapid, batch-oriented, low-cost and non-toxic leafcrushing method for a large number of molecular marker tests, improving test efficiency.
基金Supported by the Special Project of Guizhou Academy of Agricultural Sciences(Qian Nong Ke Yuan Yuan Zhuan Xiang[2016]030)the Action Plan Project of Services for Enterprises by the Science and Technology Departments of Guizhou Province(Qian Ke He Ping Tai Ren Cai[2016]5713)the Science and Technology Planning Project of Guizhou Province(Qian Ke He Zhi Cheng[2018]2376-1)~~
文摘Corn silage is the most popular and important source of energy and fber for ruminants. Much research focused on the chopping length, additives, sealing system, harmful bacteria and their toxins, and so forth, has been done on corn silage both at home in China and abroad. Some suggestions were presented in this review： （1） Corn silage modulation： Whole-plant corn harvested at the milk-ripe stage with a dry matter （DM） content of 30%~40% was selected as silage material and treated by rubbing and cutting. In the process of silage, commercial Lactobacillus buchneri was inoculated, exogenous protease added appropriately, cellulose enzyme added depending on the phenological period, the silage container and the species of the animals, and coextruded polyethylene？polyamide oxygen barrier flm （OB） selected as the seal material. （2） Strengthening management： Aerobic exposure should be avoided at the storage stage and reduced during the feeding phase. The proportion of corn silage was increased depending on the type of animal and their management. （3） Suggestions for further research： Further studies would be needed for the effects of liquid and solid wastes on soil, water and vegetation, and the treatment of these wastes.
