[Objective]The paper was to establish simple and effective method to screen marker gene in maize with kanamycin resistance.[Method]Using inbred line "Chang 7-2" and hybrid "Zhengdan 958" of maize a...[Objective]The paper was to establish simple and effective method to screen marker gene in maize with kanamycin resistance.[Method]Using inbred line "Chang 7-2" and hybrid "Zhengdan 958" of maize as test materials,their seeds were soaked with different concentrations and volumes of kanamycin for3 and 4 d,respectively,the rate of albino seedlings and average seedling height after sowing for10 d were investigated.[Result]The rate of albino seedlings not only was related to kanamycin concentration,but also had relationship with solution volume during soaking process.The difference between inbred line and hybrid was no significant.When 100 ml of kanamycin solution with concentration of 200 mg/L was used to soak seeds for 3 d,the rate of albino seedlings basically could reach 100%.When 100 ml of kanamycin solution with concentration of 100 mg/L was used to soak 20 seeds for 3 d to carry out resistance screening,the accuracy was up to 84.8% after verifying the screening test of T1 transgenic maize plants.[Conclusion]The method was feasible,which could be used as a simple method for screening transgenic gene maize with kanamycin resistance.展开更多
[Objective] This study aimed to select suitable varieties of maize for Ailao mountainous areas, [Method] A comparison test of eight varieties of maize was conducted in Shengli Village, Mosha Town, Xinping County, Yunn...[Objective] This study aimed to select suitable varieties of maize for Ailao mountainous areas, [Method] A comparison test of eight varieties of maize was conducted in Shengli Village, Mosha Town, Xinping County, Yunnan Province from May to October in 2016. [Result] The yield of Shenyu 10 was up to 66.3.4 kg/ 667 m^2 and there were extremely significant differences between Shenyu 10 and Xuanhuangdan 5 （CK）. Moreover, it had strong stress resistance. As Jingdan 12, Beiju 0913, Ante 8000, Yunrui 89 and Yayu 78 were planted in the region, their earing should not appear in rainy months. The inciderce of stem rot and ear rot of Yayu 891 was high, so Yayu 891 should not be planted in the region. [Conclusion] Shenyu 10 had a high yield, suffered few diseases and had strong stress resistance, so it can be popularized widely in the region.展开更多
基金Supported by Major Projects for Cultivation of New Varieties ofGenetically Modified Organisms (2009ZX08010-011B)
文摘[Objective]The paper was to establish simple and effective method to screen marker gene in maize with kanamycin resistance.[Method]Using inbred line "Chang 7-2" and hybrid "Zhengdan 958" of maize as test materials,their seeds were soaked with different concentrations and volumes of kanamycin for3 and 4 d,respectively,the rate of albino seedlings and average seedling height after sowing for10 d were investigated.[Result]The rate of albino seedlings not only was related to kanamycin concentration,but also had relationship with solution volume during soaking process.The difference between inbred line and hybrid was no significant.When 100 ml of kanamycin solution with concentration of 200 mg/L was used to soak seeds for 3 d,the rate of albino seedlings basically could reach 100%.When 100 ml of kanamycin solution with concentration of 100 mg/L was used to soak 20 seeds for 3 d to carry out resistance screening,the accuracy was up to 84.8% after verifying the screening test of T1 transgenic maize plants.[Conclusion]The method was feasible,which could be used as a simple method for screening transgenic gene maize with kanamycin resistance.
文摘[Objective] This study aimed to select suitable varieties of maize for Ailao mountainous areas, [Method] A comparison test of eight varieties of maize was conducted in Shengli Village, Mosha Town, Xinping County, Yunnan Province from May to October in 2016. [Result] The yield of Shenyu 10 was up to 66.3.4 kg/ 667 m^2 and there were extremely significant differences between Shenyu 10 and Xuanhuangdan 5 （CK）. Moreover, it had strong stress resistance. As Jingdan 12, Beiju 0913, Ante 8000, Yunrui 89 and Yayu 78 were planted in the region, their earing should not appear in rainy months. The inciderce of stem rot and ear rot of Yayu 891 was high, so Yayu 891 should not be planted in the region. [Conclusion] Shenyu 10 had a high yield, suffered few diseases and had strong stress resistance, so it can be popularized widely in the region.
