High-performance liquid chromatography with UV detection was used for the quantification of the flavonoid quercetin, the active compound found in "Guangdong Wang-bu-liu-xing". The method was developed and demonstrat...High-performance liquid chromatography with UV detection was used for the quantification of the flavonoid quercetin, the active compound found in "Guangdong Wang-bu-liu-xing". The method was developed and demonstrated to provide superior performance over other commonly documented methods. This HPLC assay achieved high specificity through the use of a reversed-phase C18 column eluted with a mobile phase of methanol-0.4% (v/v) phosphoric acid (50:50, v/v) over the course of 30 min. UV detection at 360 nm was used. This analytical method provided excellent precision and a mean recovery of 99%, demonstrated by repeated analysis of 11 sample groups. Because of its high performance and simplicity, this HPLC assay can be readily utilized as a practical method for the quality control of active compounds extracted from Guangdong Wang-bu-liu-xing.展开更多
Objective To compare the differences of effect on infantile amblyopia treated with auricular point sticking therapy and regular comprehensive therapy of western medicine.Methods Two hundreds and thirty cases of amblyo...Objective To compare the differences of effect on infantile amblyopia treated with auricular point sticking therapy and regular comprehensive therapy of western medicine.Methods Two hundreds and thirty cases of amblyopia patients were randomly divided into two groups,namely the group of auricular point sticking therapy,which had 120 cases(212 eyes),and the group of comprehensive therapy,which had 110 cases(194 eyes).The seed of Semen Vaccariae was applied at the ear point such as the eye,ear Shenmen,the liver,the spleen and the kidney.Regular comprehensive therapy of western medicine included covering method,vision corrected with glasses and fine exercise.Vision improvements of the two groups were observed with a three-year follow up.Results The effective rates of the auricular point sticking therapy group and comprehensive therapy group of ametropic amblyopia were 81.0%(64/79) and 52.2%(36/69),respectively.The effective rates of anisometropic amblyopia of the two groups were 73.1%(49/67) and 47.7%(31/65),respectively.And the effective rates of the two groups of strabismic amblyopia were 71.2%(47/66) and 45.0%(27/60),respectively.The therapeutic effects of auricular point sticking therapy on all kinds of amblyopia were better than those of the comprehensive therapy(all P0.05).Conclusion The effect of auricular point sticking therapy on infantile amblyopia is better than that of regular comprehensive therapy of western medicine,which can obviously improve the vision of the patients with easy manipulations.展开更多
OBJECTIVE:To observe the effect of pressing auricular points on myopia in children.METHODS:Myopic children aged 8-9 years were divided into two groups.The Chinese herb Semen Vaccariae was used to stimulate the auricul...OBJECTIVE:To observe the effect of pressing auricular points on myopia in children.METHODS:Myopic children aged 8-9 years were divided into two groups.The Chinese herb Semen Vaccariae was used to stimulate the auricular points of CO_(10),LO_5,TF_4,CO_(15),CO_(12),and CO_(13) for three treatment courses for the experimental group(n =39),while the same therapy was not given to the control group(n = 33).Binocular naked vision of the experimental group and the control group before and after the intervention was measured using a standard E visual acuity chart.RESULTS:There was no difference in the binocular naked vision between the experimental group and the control group before the intervention.In contrast,there was significant improvement in binocular naked vision in the experimental group after the intervention compared with the control group(P <0.05).CONCLUSION:When combined with external medical application,non-invasive auricular therapy could improve the function of myopia in children.Auricular pressing was well received by the children.展开更多
文摘High-performance liquid chromatography with UV detection was used for the quantification of the flavonoid quercetin, the active compound found in "Guangdong Wang-bu-liu-xing". The method was developed and demonstrated to provide superior performance over other commonly documented methods. This HPLC assay achieved high specificity through the use of a reversed-phase C18 column eluted with a mobile phase of methanol-0.4% (v/v) phosphoric acid (50:50, v/v) over the course of 30 min. UV detection at 360 nm was used. This analytical method provided excellent precision and a mean recovery of 99%, demonstrated by repeated analysis of 11 sample groups. Because of its high performance and simplicity, this HPLC assay can be readily utilized as a practical method for the quality control of active compounds extracted from Guangdong Wang-bu-liu-xing.
文摘Objective To compare the differences of effect on infantile amblyopia treated with auricular point sticking therapy and regular comprehensive therapy of western medicine.Methods Two hundreds and thirty cases of amblyopia patients were randomly divided into two groups,namely the group of auricular point sticking therapy,which had 120 cases(212 eyes),and the group of comprehensive therapy,which had 110 cases(194 eyes).The seed of Semen Vaccariae was applied at the ear point such as the eye,ear Shenmen,the liver,the spleen and the kidney.Regular comprehensive therapy of western medicine included covering method,vision corrected with glasses and fine exercise.Vision improvements of the two groups were observed with a three-year follow up.Results The effective rates of the auricular point sticking therapy group and comprehensive therapy group of ametropic amblyopia were 81.0%(64/79) and 52.2%(36/69),respectively.The effective rates of anisometropic amblyopia of the two groups were 73.1%(49/67) and 47.7%(31/65),respectively.And the effective rates of the two groups of strabismic amblyopia were 71.2%(47/66) and 45.0%(27/60),respectively.The therapeutic effects of auricular point sticking therapy on all kinds of amblyopia were better than those of the comprehensive therapy(all P0.05).Conclusion The effect of auricular point sticking therapy on infantile amblyopia is better than that of regular comprehensive therapy of western medicine,which can obviously improve the vision of the patients with easy manipulations.
基金the Research Project of the State Administration of Traditional Chinese Medicine(Evaluation on Treatment of Teenagers with Four-section Therapy,No.ZZ03116)
文摘OBJECTIVE:To observe the effect of pressing auricular points on myopia in children.METHODS:Myopic children aged 8-9 years were divided into two groups.The Chinese herb Semen Vaccariae was used to stimulate the auricular points of CO_(10),LO_5,TF_4,CO_(15),CO_(12),and CO_(13) for three treatment courses for the experimental group(n =39),while the same therapy was not given to the control group(n = 33).Binocular naked vision of the experimental group and the control group before and after the intervention was measured using a standard E visual acuity chart.RESULTS:There was no difference in the binocular naked vision between the experimental group and the control group before the intervention.In contrast,there was significant improvement in binocular naked vision in the experimental group after the intervention compared with the control group(P <0.05).CONCLUSION:When combined with external medical application,non-invasive auricular therapy could improve the function of myopia in children.Auricular pressing was well received by the children.