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柱晶白霉素产生菌的原生质体制备、再生和诱变 被引量:6
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作者 倪孟祥 刘娜 +1 位作者 顾觉奋 吴梧桐 《中国药科大学学报》 CAS CSCD 北大核心 2001年第6期462-467,共6页
目的 :对柱晶白霉素产生菌 SK-0 6的原生质体制备和再生的条件 ,以及再生菌的产生抗生素能力进行了研究。方法 :考察甘氨酸浓度对菌丝生长的影响 ,摸索和确定溶菌酶酶解的最佳条件 ,考察再生培养基的组成。结果 :溶菌酶酶解的最佳条件为... 目的 :对柱晶白霉素产生菌 SK-0 6的原生质体制备和再生的条件 ,以及再生菌的产生抗生素能力进行了研究。方法 :考察甘氨酸浓度对菌丝生长的影响 ,摸索和确定溶菌酶酶解的最佳条件 ,考察再生培养基的组成。结果 :溶菌酶酶解的最佳条件为 :2 mg/ml、30℃、90 min。再生率达到 1 5 %。通过二次再生后 ,获得了一株高产菌株 ,效价提高了 2 38%。经过紫外线诱变的孢子和原生质体的效价提高了 95 %和 1 6 0 %。结论 :原生质体制备和再生选育高产菌株是一种有效的菌种选育手段。 展开更多
关键词 柱晶白霉素 原生质体形成 原生质体再生 紫外线 诱变 抗生素 环内酯类抗素
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Research Progress of Ketolide Antibiotics
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作者 邵莉萍 张继瑜 《Agricultural Science & Technology》 CAS 2017年第8期1474-1479,共6页
Macrolide antibiotics have been widely used in clinic due to their good antibacterial effects and high safety, but the drug-resistant bacteria appear constantly. To solve the problem of drug resistance in pathogens, s... Macrolide antibiotics have been widely used in clinic due to their good antibacterial effects and high safety, but the drug-resistant bacteria appear constantly. To solve the problem of drug resistance in pathogens, scholars obtain the third generation of macrolide antibiotics, ketolide antibiotics, which are developed by modifying the structure of macrolide antibiotics, thereby efficiently solving the problem. Ketolide antibiotics are a type of erythromycin derivatives with macrolide structure, and the typical drugs mainly include telithromycin, cethromycin and solithromycin, etc . This paper briefly introduced the recent progress of ketolide antibiotics, with an attempt to provide help to the research and development of new macrolide antibiotics. 展开更多
关键词 Macrolide antibiotics Ketolide antibiotics TELITHROMYCIN Cethromycin Solithromycin PROGRESS
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Selection of Reference Genes in Saccharopolyspora Spinosa for Real-Time PCR
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作者 张传波 薛超友 +1 位作者 申月琪 卢文玉 《Transactions of Tianjin University》 EI CAS 2015年第5期461-467,共7页
Reverse transcription quantitative PCR (RT-qPCR) combined with the published genome information of Saccharopolyspora spinosa can allow sophisticated studies about S. spinosa, including Studying the regulation of spi... Reverse transcription quantitative PCR (RT-qPCR) combined with the published genome information of Saccharopolyspora spinosa can allow sophisticated studies about S. spinosa, including Studying the regulation of spinosyn biosynthesis, finding new target genes for engineering, and discovering and exploiting other macrolide secondary metabolites. Studies have demonstrated that appropriate internal control is needed to normalize target genes at transcription levels. However, many studies have shown that no single reference gene is universal for all strains under all experimental conditions. Thus, eight candidate reference genes of three different S. spinosa strains in two different cultures were studied to find suitable reference gene(sl. The number of amplification cycles of these candidate genes was calculated by BestKeeper, NormFinder and geNorm. The results indicated that the most suitable reference genes for normalization during the fermentation of S. spinosa were 16S rRNA and rbL13. 展开更多
关键词 real-time PCR reference genes SaecharopOlysporaspinosa
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