Some key factors on the heavy metals removal efficiencies were studied when soil washing technology was used in the remediation of soils contaminated by multiple heavy metals. The results show that the dissolubilities...Some key factors on the heavy metals removal efficiencies were studied when soil washing technology was used in the remediation of soils contaminated by multiple heavy metals. The results show that the dissolubilities of Cu and Zn are promoted by humic acids, but Pb and Cd are inhibited by humic acids; heavy metals in the clay are more difficult to be extracted than silt; the strong acidic soils can cause the protonation of EDTA and weaken its extracting ability; EDTA is effective for extracting Pb and Cd, while oxalate (OX) is effective for extracting Cu and Zn; and biosurfactant can be used as additive to improve the removal of some particular heavy metals.展开更多
Organic solid and liquid wastes contain large amounts of energy, nutrients, and water, and should not be perceived as merely waste. Recycling, composting, and combustion of non-recyclables have been practiced for deca...Organic solid and liquid wastes contain large amounts of energy, nutrients, and water, and should not be perceived as merely waste. Recycling, composting, and combustion of non-recyclables have been practiced for decades to capture the energy and values from municipal solid wastes. Treatment and disposal have been the primary management strategy for wastewater. As new technologies are emerging, alternative options for the utilization of both solid wastes and wastewater have become available. Considering the complexity of the chemical, physical, and biological properties of these wastes, multiple technologies may be required to maximize the energy and value recovery from the wastes. For this purpose, biorefin- ing tends to be an appropriate approach to completely utilize the energy and value available in wastes. Research has demonstrated that non-recyclable waste materials and bio-solids can be converted into usable heat, electricity, fuel, and chemicals through a variety of processes, and the liquid waste streams have the potential to support crop and algae growth and provide other energy recovery and food production options. In this paper, we propose new biorefining schemes aimed at organic solid and liquid wastes from municipal sources, food and biological processing plants, and animal production facilities. Four new breakthrough technologies-namely, vacuum-assisted thermophilic anaerobic digestion, extended aquaponics, oily wastes to biodiesel via glycerolysis, and microwave-assisted thermochemical conversion-can be incorporated into the biorefining schemes, thereby enabling the complete utilization of those wastes for the production of chemicals, fertilizer, energy （biogas, syngas, biodiesel, and bio-oil）, foods, and feeds, and resulting in clean water and a significant reduction in pollutant emissions.展开更多
A method based on PCR amplification of the 16S rRNA gene (rDNA)-23S rDNA intergenic spacer regions (ISR) was developed for the identification of species within the novel group hydrogen-producing anaerobes. The sizes o...A method based on PCR amplification of the 16S rRNA gene (rDNA)-23S rDNA intergenic spacer regions (ISR) was developed for the identification of species within the novel group hydrogen-producing anaerobes. The sizes of the PCR products varied from 1264 to 398 bp. Strain of isolate Rennanqilyf 3 was characterized as having products of 1262,398,638,437 and 436 bp. The isolate Rennanqilyf 1 had product of 1264 bp. The isolate Rennanqilyf 13 had products of 1261,579 and 485 bp. Of the 3 species of the novel group hydrogen-producing anaerobes examined, no one was indistinguishable. Two environmental isolates were identified as hydrogen-producing bacteria, which were new species in present taxon. Rennanqilyf 3 could not be associated with any Clostridium sp. studied. Rennanqilyf 1 could be classified into Clostridium genus. The combination between 16S rDNA equencing and length polymorphisms of IRS in 16S-23S rDNA is a better method for determining species of the hydrogen-producing bacteria.展开更多
Enhanced biological phosphorus removal(EBPR) was investigated in an anaerobic/aerobic sequencing batch reactor(SBR) supplied with glucose as a single organic substrate.The results illustrated that EBPR process could a...Enhanced biological phosphorus removal(EBPR) was investigated in an anaerobic/aerobic sequencing batch reactor(SBR) supplied with glucose as a single organic substrate.The results illustrated that EBPR process could also occur successfully with glucose other than short chain fatty acids(SCFAs).High phosphorus release and poly-hydroxyalkanoate(PHA) accumulation in the anaerobic phase was found vital for the removal of phosphorus during the aerobic phase.The measurement of intracellular reserves revealed that glycogen had a higher chance to replace the energy role of poly-P under anaerobic conditions.Moreover,glycogen was also utilized as the carbon source for PHA synthesis,as well as a reducing power as reported earlier.The accumulated PHA in this system was mainly in the form of poly-hydroxyvalerate(PHV) instead of poly-hydroxybutyrate(PHB),and was inferred to be caused by the excess reducing power contained in glucose.Lactate as a fermentation product was also found released into the bulk solution.Applying fundamental biochemistry knowledge to the experimental results,a conceptual biochemical model was developed to explain the metabolism of the glucose-induced EBPR.展开更多
Bioremediation, is an effective and environment-friendly method of cleaning up crude oil pollution after an oil spill. However, the in situ bioremediation of crude oil is usually inhibited by deficiency of inorganic n...Bioremediation, is an effective and environment-friendly method of cleaning up crude oil pollution after an oil spill. However, the in situ bioremediation of crude oil is usually inhibited by deficiency of inorganic nutrients. To understand the effects of nutrient addition on the bioremediation of crude oil and the succession of bacterial communities during process of bioremediation, microcosms containing oilcontaminated sediments were constructed and biodegradation of crude oil was assessed based on the depletion of different ingredients. We used two culture-independent methods, denaturing gradient gel electrophoresis and a 16 S rRNA gene based clone library, to analyze the succession of bacterial communities. The results suggested n-alkanes were degraded after 30 days and that nutrient amendments significantly improved the efficiency of their biodegradation. Moreover, oil contamination and nutrient amendments could dramatically change bacterial community structures. Lower diversity was detected after being contaminated with oil. For instance, bacterial clones affiliated with the phylum Armatimonadetes, Firmicutes, Gemmatimonadetes, and Planctomycetes and the class Deltaproteobacteria and Epsilonproteobacteria could not be identified after 30 days of incubation with crude oil. However, "professional hydrocarbonocastic bacteria" became abundant in samples treated with oil during the bioremediation period, while these clones were almost completely absent from the control plots. Interestingly, bioinformatics analysis showed that even when dramatic differences in oil biodegradation efficiency were observed, bacterial communities in the plots with nutrient amendments were not significantly different from those in plots treated with oil alone. These findings indicated that nutrient amendments could stimulate the process of biodegradation but had less impact on bacterial communities. Overall, nutrient amendments might be able to stimulate the growth of n-alkane degrading bacteria.展开更多
Mercury pollution in the Three Gorges Reservoir area of P.R.China merits special attention.We investigated into the current situation in the Chongqing part of the Reservoir area,identified the pollution sources and pr...Mercury pollution in the Three Gorges Reservoir area of P.R.China merits special attention.We investigated into the current situation in the Chongqing part of the Reservoir area,identified the pollution sources and proposed some suggestions for the remediation and prevention of mercury pollution in this area.Atmospheric mercury in Chongqing was mainly from coal burning and releases of mercury-containing products such as various types of lights and fever thermometers.Urban drainage in Chongqing and Changshou,and runoffs from the high mercury background area in the lower reaches of the Wujiang River contributed most of the mercury in the water of the Yangtze River.A majority of the blame should be laid on mercury and gold mining in the Wujiang valley.We suggested foresting sloping lands to relieve soil erosion and prevent mercury-bearing soil from running into rivers,educational activities to discourage use of mercury-containing products and improved infrastructure to collect mercury-containing wastes for reducing mercury releases,more facilities for treating wastewater and solid waste to accommodate increased requirements of discharge,and growing selected perennial plants in mercury-contaminated land to absorb the mercury in soil.We also suggested concerted operation of a dedicated water-quality monitoring system,reinforced legislation and an effective administrative mechanism to ensure lasting efforts are invested in curbing mercury releases and restoring mercury contaminated land and water in the Reservoir area.展开更多
基金Project(20050532009) supported by the Doctoral Foundation of Ministry of Education of ChinaProjects(2006BAD03A1704, 2006BAD03A1706) supported by the National 11th-Five Technology Supporting Project
文摘Some key factors on the heavy metals removal efficiencies were studied when soil washing technology was used in the remediation of soils contaminated by multiple heavy metals. The results show that the dissolubilities of Cu and Zn are promoted by humic acids, but Pb and Cd are inhibited by humic acids; heavy metals in the clay are more difficult to be extracted than silt; the strong acidic soils can cause the protonation of EDTA and weaken its extracting ability; EDTA is effective for extracting Pb and Cd, while oxalate (OX) is effective for extracting Cu and Zn; and biosurfactant can be used as additive to improve the removal of some particular heavy metals.
基金Department of Transport/Sun GrantUS Department of Agriculture/ Department of Energy+4 种基金Minnesota Legislative-Citizen Commission on Minnesota ResourcesMetropolitan Council Environmental ServicesUniversity of Minnesota MNDrive programsUniversity of Minnesota Center for BiorefiningChina Scholarship Council (CSC) for their financial support for this work
文摘Organic solid and liquid wastes contain large amounts of energy, nutrients, and water, and should not be perceived as merely waste. Recycling, composting, and combustion of non-recyclables have been practiced for decades to capture the energy and values from municipal solid wastes. Treatment and disposal have been the primary management strategy for wastewater. As new technologies are emerging, alternative options for the utilization of both solid wastes and wastewater have become available. Considering the complexity of the chemical, physical, and biological properties of these wastes, multiple technologies may be required to maximize the energy and value recovery from the wastes. For this purpose, biorefin- ing tends to be an appropriate approach to completely utilize the energy and value available in wastes. Research has demonstrated that non-recyclable waste materials and bio-solids can be converted into usable heat, electricity, fuel, and chemicals through a variety of processes, and the liquid waste streams have the potential to support crop and algae growth and provide other energy recovery and food production options. In this paper, we propose new biorefining schemes aimed at organic solid and liquid wastes from municipal sources, food and biological processing plants, and animal production facilities. Four new breakthrough technologies-namely, vacuum-assisted thermophilic anaerobic digestion, extended aquaponics, oily wastes to biodiesel via glycerolysis, and microwave-assisted thermochemical conversion-can be incorporated into the biorefining schemes, thereby enabling the complete utilization of those wastes for the production of chemicals, fertilizer, energy （biogas, syngas, biodiesel, and bio-oil）, foods, and feeds, and resulting in clean water and a significant reduction in pollutant emissions.
基金Sponsored by Program of Shanghai Education Committee (Grant No07ZZ156)Key Subject Construction of Shanghai Education Committee(Grant NoP1402) the National Natural Science Fund of China(Grant No30470054)
文摘A method based on PCR amplification of the 16S rRNA gene (rDNA)-23S rDNA intergenic spacer regions (ISR) was developed for the identification of species within the novel group hydrogen-producing anaerobes. The sizes of the PCR products varied from 1264 to 398 bp. Strain of isolate Rennanqilyf 3 was characterized as having products of 1262,398,638,437 and 436 bp. The isolate Rennanqilyf 1 had product of 1264 bp. The isolate Rennanqilyf 13 had products of 1261,579 and 485 bp. Of the 3 species of the novel group hydrogen-producing anaerobes examined, no one was indistinguishable. Two environmental isolates were identified as hydrogen-producing bacteria, which were new species in present taxon. Rennanqilyf 3 could not be associated with any Clostridium sp. studied. Rennanqilyf 1 could be classified into Clostridium genus. The combination between 16S rDNA equencing and length polymorphisms of IRS in 16S-23S rDNA is a better method for determining species of the hydrogen-producing bacteria.
基金Science and Technology Project of Zhejiang and Hangzhou (No2007C13081, No20062912A06)
文摘Enhanced biological phosphorus removal(EBPR) was investigated in an anaerobic/aerobic sequencing batch reactor(SBR) supplied with glucose as a single organic substrate.The results illustrated that EBPR process could also occur successfully with glucose other than short chain fatty acids(SCFAs).High phosphorus release and poly-hydroxyalkanoate(PHA) accumulation in the anaerobic phase was found vital for the removal of phosphorus during the aerobic phase.The measurement of intracellular reserves revealed that glycogen had a higher chance to replace the energy role of poly-P under anaerobic conditions.Moreover,glycogen was also utilized as the carbon source for PHA synthesis,as well as a reducing power as reported earlier.The accumulated PHA in this system was mainly in the form of poly-hydroxyvalerate(PHV) instead of poly-hydroxybutyrate(PHB),and was inferred to be caused by the excess reducing power contained in glucose.Lactate as a fermentation product was also found released into the bulk solution.Applying fundamental biochemistry knowledge to the experimental results,a conceptual biochemical model was developed to explain the metabolism of the glucose-induced EBPR.
基金Supported by the Hundred Talents Program of the Chinese Academy of Sciences awarded to Dr.Xiaoke HUthe Key Research Program of Chinese Academy of Sciences(No.KZZD-EW-14)+1 种基金the Science and Technology Program of Shandong Province(No.2013GHY11534)the National Natural Science Foundation of China(Nos.41376138,41576165)
文摘Bioremediation, is an effective and environment-friendly method of cleaning up crude oil pollution after an oil spill. However, the in situ bioremediation of crude oil is usually inhibited by deficiency of inorganic nutrients. To understand the effects of nutrient addition on the bioremediation of crude oil and the succession of bacterial communities during process of bioremediation, microcosms containing oilcontaminated sediments were constructed and biodegradation of crude oil was assessed based on the depletion of different ingredients. We used two culture-independent methods, denaturing gradient gel electrophoresis and a 16 S rRNA gene based clone library, to analyze the succession of bacterial communities. The results suggested n-alkanes were degraded after 30 days and that nutrient amendments significantly improved the efficiency of their biodegradation. Moreover, oil contamination and nutrient amendments could dramatically change bacterial community structures. Lower diversity was detected after being contaminated with oil. For instance, bacterial clones affiliated with the phylum Armatimonadetes, Firmicutes, Gemmatimonadetes, and Planctomycetes and the class Deltaproteobacteria and Epsilonproteobacteria could not be identified after 30 days of incubation with crude oil. However, "professional hydrocarbonocastic bacteria" became abundant in samples treated with oil during the bioremediation period, while these clones were almost completely absent from the control plots. Interestingly, bioinformatics analysis showed that even when dramatic differences in oil biodegradation efficiency were observed, bacterial communities in the plots with nutrient amendments were not significantly different from those in plots treated with oil alone. These findings indicated that nutrient amendments could stimulate the process of biodegradation but had less impact on bacterial communities. Overall, nutrient amendments might be able to stimulate the growth of n-alkane degrading bacteria.
基金the Natural Science Foundation of China under the Grant No.20377054
文摘Mercury pollution in the Three Gorges Reservoir area of P.R.China merits special attention.We investigated into the current situation in the Chongqing part of the Reservoir area,identified the pollution sources and proposed some suggestions for the remediation and prevention of mercury pollution in this area.Atmospheric mercury in Chongqing was mainly from coal burning and releases of mercury-containing products such as various types of lights and fever thermometers.Urban drainage in Chongqing and Changshou,and runoffs from the high mercury background area in the lower reaches of the Wujiang River contributed most of the mercury in the water of the Yangtze River.A majority of the blame should be laid on mercury and gold mining in the Wujiang valley.We suggested foresting sloping lands to relieve soil erosion and prevent mercury-bearing soil from running into rivers,educational activities to discourage use of mercury-containing products and improved infrastructure to collect mercury-containing wastes for reducing mercury releases,more facilities for treating wastewater and solid waste to accommodate increased requirements of discharge,and growing selected perennial plants in mercury-contaminated land to absorb the mercury in soil.We also suggested concerted operation of a dedicated water-quality monitoring system,reinforced legislation and an effective administrative mechanism to ensure lasting efforts are invested in curbing mercury releases and restoring mercury contaminated land and water in the Reservoir area.
