Choosing a strategy for sustainable development is national level aimed at the simultaneous fulfillment of economic, a fundamental problem that requires non-trivial approaches at the social and environmental goals. Th...Choosing a strategy for sustainable development is national level aimed at the simultaneous fulfillment of economic, a fundamental problem that requires non-trivial approaches at the social and environmental goals. This contradiction is possible to be adequately described in the complex game-theoretic models. But analysis of them is complicated by the problems of algorithmic solvability and the payoff functions construction. Also, the volume of data required creates considerable complexity increased by the conditions of situations uncertainty and conflict. At the same time, international organizations, such as UN （United Nations）, ITU （International Telecommunications Union）, are widely used index system. Indices, built on the aggregation of elementary indicators, allow the estimation of the components of the system and its overall development. The paper discusses the basic concepts of game-theoretic approach to the problems of finding strategies for sustainable development, using indicative analysis techniques. In the proposed approach, estimating of sustainability is considered as the result of finding the optimal values of the payoff functions in general game-theoretical model where the players are matched indices. The interests of the player are to increase of the corresponding index and the optimal situation is implemented as a set of optimal payoff functions values. This allows switching from processing of large amounts of data describing the behavior of the ecological-economic system by the aid of indicators to aggregate estimations, which significantly reduce the sample, and also facilitates the interpretation of the results.展开更多
Along with the deepening of reform and opening up, the impact of diversified culture, changing educational environment, emergencies occur ~equently in university, which seriously affected the normal teaching and manag...Along with the deepening of reform and opening up, the impact of diversified culture, changing educational environment, emergencies occur ~equently in university, which seriously affected the normal teaching and management order in university. This article analyzesthe concept, characteristics, causes and linkage system of university emergencies.展开更多
Objective： Cancer-associated fibroblasts （CAFs） are one of the hallmarks of the cancer microenvironment. Recent evidence has indicated that CAFs are more competent in enhancing cancer cell growth and migration than...Objective： Cancer-associated fibroblasts （CAFs） are one of the hallmarks of the cancer microenvironment. Recent evidence has indicated that CAFs are more competent in enhancing cancer cell growth and migration than normal fibroblasts. However, the unique protein expression of CAFs has not been fully elucidated. This study aims to investigate the characterizations of colon CAFs by comparing the differential protein expression between CAFs and normal fibroblasts. Methods： Primary fibroblasts were isolated from surgical specimen of human colon cancer and matched normal colonic tissue. Purity of the cell population was verified through immunostain analysis. Total cell lysates and conditioned media from each group of cells were extracted, and protein expression analysis was con- ducted using the surface-enhanced laser desorption/ionization time-of-flight mass spectrometry （SELDI-TOF-MS） ProteinChip platform. Results： Most primary cells showed typical fibroblast-like features after two weeks. Increased proportion of a-smooth muscle actin-positive myofibroblasts was detected within the CAFs in four of the six pairs of primary cells. Fibroblast activation protein was weakly expressed in most cells without differences. Using SELDI-TOF-MS ProteinChip platform, four protein peaks mass over charge ratio （m/z） 1142, 3011, 4035, and 4945 were detected in the total cell lysates, and two protein peaks m/z 1368 and 1389 were detected in the conditioned media. The potential candidate proteins found in the Swiss-Prot database include morphogenetic neuropeptides, FMRFamide-related peptides, insulin-like growth factor II, thymosin 13-4-like protein 3, and tight junction-associated protein 1. Conclusions： Using the SELDI-ProteinChip platform, differential protein expressions were identified in colon CAFs compared with normal colonic stromal fibroblasts. The complex proteomic alternations in colon CAFs may play important roles related to the colon cancer microenvironment.展开更多
文摘Choosing a strategy for sustainable development is national level aimed at the simultaneous fulfillment of economic, a fundamental problem that requires non-trivial approaches at the social and environmental goals. This contradiction is possible to be adequately described in the complex game-theoretic models. But analysis of them is complicated by the problems of algorithmic solvability and the payoff functions construction. Also, the volume of data required creates considerable complexity increased by the conditions of situations uncertainty and conflict. At the same time, international organizations, such as UN （United Nations）, ITU （International Telecommunications Union）, are widely used index system. Indices, built on the aggregation of elementary indicators, allow the estimation of the components of the system and its overall development. The paper discusses the basic concepts of game-theoretic approach to the problems of finding strategies for sustainable development, using indicative analysis techniques. In the proposed approach, estimating of sustainability is considered as the result of finding the optimal values of the payoff functions in general game-theoretical model where the players are matched indices. The interests of the player are to increase of the corresponding index and the optimal situation is implemented as a set of optimal payoff functions values. This allows switching from processing of large amounts of data describing the behavior of the ecological-economic system by the aid of indicators to aggregate estimations, which significantly reduce the sample, and also facilitates the interpretation of the results.
文摘Along with the deepening of reform and opening up, the impact of diversified culture, changing educational environment, emergencies occur ~equently in university, which seriously affected the normal teaching and management order in university. This article analyzesthe concept, characteristics, causes and linkage system of university emergencies.
基金supported by the National Natural Science Foundation of China (Nos. 81000892, 81071801, and 30801341)the Research Fund for the Doctoral Program of Higher Education of China (No. 200803351107)
文摘Objective： Cancer-associated fibroblasts （CAFs） are one of the hallmarks of the cancer microenvironment. Recent evidence has indicated that CAFs are more competent in enhancing cancer cell growth and migration than normal fibroblasts. However, the unique protein expression of CAFs has not been fully elucidated. This study aims to investigate the characterizations of colon CAFs by comparing the differential protein expression between CAFs and normal fibroblasts. Methods： Primary fibroblasts were isolated from surgical specimen of human colon cancer and matched normal colonic tissue. Purity of the cell population was verified through immunostain analysis. Total cell lysates and conditioned media from each group of cells were extracted, and protein expression analysis was con- ducted using the surface-enhanced laser desorption/ionization time-of-flight mass spectrometry （SELDI-TOF-MS） ProteinChip platform. Results： Most primary cells showed typical fibroblast-like features after two weeks. Increased proportion of a-smooth muscle actin-positive myofibroblasts was detected within the CAFs in four of the six pairs of primary cells. Fibroblast activation protein was weakly expressed in most cells without differences. Using SELDI-TOF-MS ProteinChip platform, four protein peaks mass over charge ratio （m/z） 1142, 3011, 4035, and 4945 were detected in the total cell lysates, and two protein peaks m/z 1368 and 1389 were detected in the conditioned media. The potential candidate proteins found in the Swiss-Prot database include morphogenetic neuropeptides, FMRFamide-related peptides, insulin-like growth factor II, thymosin 13-4-like protein 3, and tight junction-associated protein 1. Conclusions： Using the SELDI-ProteinChip platform, differential protein expressions were identified in colon CAFs compared with normal colonic stromal fibroblasts. The complex proteomic alternations in colon CAFs may play important roles related to the colon cancer microenvironment.
