当归/黄芪多糖对腹腔注射环磷酰胺小鼠骨髓造血影响随机平行对照研究

[目的]观察当归/黄芪多糖对腹腔注射环磷酰胺小鼠骨髓造血影响。[方法]使用随机平行对照方法,将60只清洁级C57BL/6小鼠编号按随机数字表法随机分为6组,空白组、模型组、促红细胞生成素组(EPO组)、当归多糖组(当归组)、黄芪多糖组(黄芪组)、当归加黄芪多糖组(当归+黄芪组),10只/组。实验第1d,各组小鼠称体重,模型组、EPO组、当归组、黄芪组、当归+黄芪组,腹腔注射环磷酰胺380mg/kg·d^(-1),连续3d,空白组注射等量生理盐水。实验第4d(造模第4d),颈椎脱臼处死小鼠,无菌取股骨和胫骨,置入培养基中,调整细胞浓度为1×10~5/m L,然后将混合液加入96孔板(200u L)/孔和24孔板(1L/孔)中,每组设3个复孔,周围孔分别加入相应量的无菌超纯水,将细胞板置于5%CO_2,37℃孵育箱中培养。实验第4d(造模第4d),各组分别干预。观测外周血象、细胞增殖率(MTT法),EPO、IL-3(免疫蛋白法),细胞内转录因子JAK2、STAT5(RT-PCR法)。[结果]细胞培养24h、48h和72h,细胞增殖率EPO组、当归组、黄芪组、当归+黄芪组均高于空白组(P<0.05,P<0.01),组间无显著差异(P>0.05);模型组低于空白组(P<0.05,P<0.01)。细胞培养7d,IL-3、EPO表达模型组低于空白组(P<0.01),各干预组均高于模型组(P<0.01),当归+黄芪组高于EPO组(P<0.01),EPO组高于当归组(P<0.01),当归组高于黄芪组(P<0.01)。细胞培养7d,单核细胞STAT5、JAK2m RNA模型组低于空白组(P<0.01),各干预组高于模型组(P<0.05或P<0.01),当归+黄芪组高于EPO组(P<0.01),EPO组与当归组无显著差异(P<0.05),当归组高于黄芪组(P<0.01)。[结论]当归/黄芪多糖、促红细胞生成素干预腹腔注射环磷酰胺小鼠,均可升高骨髓细胞增殖率、IL-3、EPO表达及单核细胞STAT5、JAK2m RNA,当归多糖与黄芪多糖联合应用更明显。

扶正升白提取膏穴贴对化疗小鼠造血和免疫功能影响的实验研究

目的:探讨扶正升白提取膏对化疗小鼠造血和免疫功能的保护机理。方法:选用昆明种小鼠腹腔注射环磷酰胺(CTX)造成骨髓抑制模型,采用穴贴扶正升白原粉膏组和模型组对照,检测外周血白细胞数、小鼠骨髓细胞分类、IL-2、IL-6等指标,并进行统计学分析。结果:穴贴扶正升白提取膏对化疗小鼠造血和免疫功能有较好的保护作用,且提取制备后的药膏作用优于原粉膏剂。

Effect of Acupuncture-moxibustion on Bone Marrow Suppression and Leukocytes after Chemotherapy

Objective： To observe the effect of acupuncture-moxibustion therapy on bone marrow suppression and the leukocytes count after chemotherapy, and to explore the mechanism. Methods： Animal experiments and clinical studies were combined to observe the effect of acupuncture-moxibustion on the bone marrow and leukocytes during chemotherapy. The biological cultivation was used to detect the amount of the hematopoietic stem/progenitor cells and the activity of the serum colony-stimulating factor （CSF）. Results： The peripheral leukocytes count in the acupuncture-moxibustion group began to rebound 1d earlier than that in the model group. The effect of acupuncture-moxibustion on neutrophils proliferation was the most obvious. Conclusion： The effect of acupuncture-moxibustion on resisting bone marrow suppression and rebounding the leukocytes count after chemotherapy may related to releasing the bone marrow cells to peripheral blood, extending the life of leukocyte, increasing the activity of serum CSF, promoting the hematopoietic stem/progenitor cell proliferation, and reducing the damage of chemotherapy on the hematopoietic stem/progenitor cell.

Effect of Zishenshengxue capsule on myelosuppression in mice induced by cyclophosphamide

OBJECTIVE:To explore the inhibitory effect of Zishenshengxue capsule(ZSC) on myelosuppression in mice induced by cyclophosphamide.METHODS:Kunming mice were randomly assigned into a control group,myelosuppression group,or groups for mice with myelosuppression receiving high dose ZSC,middle dose ZSC,low dose ZSC or Yixuesheng.Myelosuppression was induced by peritoneal injection of cyclophosphamide.ZSC and cyclophosphamide were administered simultaneously.The numbers of peripheral blood cells and bone marrow karyocytes were counted.Cell proliferation activity and colony formation of granulocyte-monocyte series hemopoietic progenitor cells(C-GMs) and cell cycle were detected.RESULTS:The numbers of white blood cells in the middle and high dose ZSC groups were significantly increased at the 12th and 13th day(P<0.01) and bone marrow karyocytes and cell proliferation activity increased in the high dose ZSC group(P<0.01) compared with the myelosuppression group.C-GMs of middle and high dose ZSC groups significantly increased(P<0.01).The percentage of G 1 phase in the high dose ZSC group decreased(P< 0.01) and the percentage of S and G 2 phase increased(P<0.01).CONCLUSION:ZSC increased the numbers of peripheral white blood cells,bone marrow karyocytes and C-GMs.ZSC also increased cell proliferation activity and removed the G 1 phase block.Thus,ZSC could reduce myelosuppression in mice induced by cyclophosphamide.