加减养精种玉汤通过Rictor/mTORC2通路改善大鼠卵巢储备功能的机制研究

目的探讨加减养精种玉汤通过雷帕霉素不敏感性伴随蛋白(rapamycin-insensitive companion of m TOR,Rictor)/哺乳动物雷帕霉素靶蛋白复合物2(mammalian targeting of rapamycin complex 2,mTORC2)通路改善大鼠卵巢储备功能的作用机制。方法准备动情周期正常的雌性SD大鼠32只,随机选取其中8只作为空白组(等剂量生理盐水灌胃),其余SD大鼠首次腹腔注射环磷酰胺50mg/mL后连续14 d腹腔注射8 mg/kg诱导卵巢储备功能减退(diminished ovarian reserve,DOR)模型。按随机数字表法将造模成功的24只大鼠分为模型组(等剂量生理盐水灌胃)、中药组(加减养精种玉汤3.39 g/kg灌胃)、卵泡刺激素(follicle stimulating hormone,FSH)组(FSH 0.105 mg/kg灌胃)。记录大鼠动情周期;HE染色观察左侧卵巢组织病理学变化并计算各组大鼠左侧卵巢窦卵泡数量;计算子宫、左侧卵巢系数;ELISA法测定FSH、黄体生成素(luteinizing hormone,LH)、雌二醇(estradiol,E_(2))、抗米勒管激素(anti-Müllerian hormone,AMH)水平;Western blot检测大鼠左侧卵巢组织Rictor、m TORC2蛋白表达水平。结果与空白组相比,模型组动情周期次数减少(P<0.05);左侧卵巢成熟卵泡少;左侧卵巢指数、子宫指数、左侧卵巢窦卵泡数量均降低(P<0.05,P<0.01);血清AMH、E_(2)降低(P<0.01),LH、FSH升高(P<0.01);左侧卵巢组织Rictor、mTORC2蛋白相对表达量升高(P<0.01)。与模型组比,中药组及FSH组左侧卵巢结构改善,各级卵泡数量增加;左侧卵巢指数、子宫指数、左侧卵巢窦卵泡数量均上升(P<0.05);血清AMH、E_(2)升高(P<0.01),LH、FSH降低(P<0.01);左侧卵巢组织Rictor、mTORC2蛋白相对表达量降低(P<0.01)。结论加减养精种玉汤能够改善激素水平、调节卵巢卵泡细胞,从而起到改善DOR的作用,其机制可能与降低Rictor/mTORC2的表达有关。

宝乐果多糖的体内免疫活性研究

目的:研究宝乐果多糖体内免疫活性。方法:54只雄性BALB/c小鼠被随机分为6组,每组9只。以80 mg/(kg·d)腹腔注射环磷酰胺诱导的免疫功能低下小鼠为实验对象,设100、200、400 mg/(kg·d)三个水平处理,饲养30 d后观察多糖对细胞免疫功能、体液免疫功能和单核巨噬细胞功能的影响。结果:腹腔注射环磷酰胺可以显著降低小鼠体内细胞免疫(P<0.05),体液免疫(P<0.01)和单核巨噬细胞(P<0.01)的功能。宝乐果多糖能改善环磷酰胺诱导的免疫力低下,灌服低、中、高剂量的多糖能显著改善小鼠体内细胞免疫功能(均为P<0.05)和单核巨噬细胞的功能(均为P<0.05)、而中、高剂量的多糖能显著提高体液免疫功能(P<0.01)。结论:宝乐果多糖能增强体内免疫力,并对环磷酰胺有一定的减毒作用。因此,宝乐果多糖可以作为一种免疫调节物质。

Effect of Zishenshengxue capsule on myelosuppression in mice induced by cyclophosphamide

OBJECTIVE:To explore the inhibitory effect of Zishenshengxue capsule(ZSC) on myelosuppression in mice induced by cyclophosphamide.METHODS:Kunming mice were randomly assigned into a control group,myelosuppression group,or groups for mice with myelosuppression receiving high dose ZSC,middle dose ZSC,low dose ZSC or Yixuesheng.Myelosuppression was induced by peritoneal injection of cyclophosphamide.ZSC and cyclophosphamide were administered simultaneously.The numbers of peripheral blood cells and bone marrow karyocytes were counted.Cell proliferation activity and colony formation of granulocyte-monocyte series hemopoietic progenitor cells(C-GMs) and cell cycle were detected.RESULTS:The numbers of white blood cells in the middle and high dose ZSC groups were significantly increased at the 12th and 13th day(P<0.01) and bone marrow karyocytes and cell proliferation activity increased in the high dose ZSC group(P<0.01) compared with the myelosuppression group.C-GMs of middle and high dose ZSC groups significantly increased(P<0.01).The percentage of G 1 phase in the high dose ZSC group decreased(P< 0.01) and the percentage of S and G 2 phase increased(P<0.01).CONCLUSION:ZSC increased the numbers of peripheral white blood cells,bone marrow karyocytes and C-GMs.ZSC also increased cell proliferation activity and removed the G 1 phase block.Thus,ZSC could reduce myelosuppression in mice induced by cyclophosphamide.