Objective: The present study was to observe the expression of COX-2 protein in the dorsal horn of the spinal cord in rats with chronic inflammatory pain to investigate whether COX-2 is involved in the mechanism of el...Objective: The present study was to observe the expression of COX-2 protein in the dorsal horn of the spinal cord in rats with chronic inflammatory pain to investigate whether COX-2 is involved in the mechanism of electroacupuncture (EA) analgesia. Methods: After EA treatment, the pain score and expression of COX-2 protein in the dorsal horn of spinal cord in rats with complete Freund's adjuvant (CFA)-induced arthritis were measured by the behavioral test and immunohistochemistry technique, and compared with those of the normal group, model group and medicine group. Results: EA could decrease the pain score significantly and repeated EA treatments had the remarkable cumulative analgesic effect; and with the increase in EA treatments, the number of COX-2 immunoreactive cells was obviously decreased. Conclusion: EA at acupoints Yanglingquan (GB 34) and Kunlun (BL 60) had remarkable analgesic effect on adjuvant arthritis rats; and in the meantime, could inhibit the expression of COX-2 protein in the dorsal horn of spinal cord.展开更多
基金Supported by Shanghai Key Project of TCM 2000(3)Shanghai Science and Technology Committee(02DZ1915001)
文摘Objective: The present study was to observe the expression of COX-2 protein in the dorsal horn of the spinal cord in rats with chronic inflammatory pain to investigate whether COX-2 is involved in the mechanism of electroacupuncture (EA) analgesia. Methods: After EA treatment, the pain score and expression of COX-2 protein in the dorsal horn of spinal cord in rats with complete Freund's adjuvant (CFA)-induced arthritis were measured by the behavioral test and immunohistochemistry technique, and compared with those of the normal group, model group and medicine group. Results: EA could decrease the pain score significantly and repeated EA treatments had the remarkable cumulative analgesic effect; and with the increase in EA treatments, the number of COX-2 immunoreactive cells was obviously decreased. Conclusion: EA at acupoints Yanglingquan (GB 34) and Kunlun (BL 60) had remarkable analgesic effect on adjuvant arthritis rats; and in the meantime, could inhibit the expression of COX-2 protein in the dorsal horn of spinal cord.