Duplications of MECP2-containing genomic segments led to severe autistic symptoms in male. Transgenic mice overexpressing the human MECP2 gene exhibit autistic-like behaviors. Neural circuits underlying social defects...Duplications of MECP2-containing genomic segments led to severe autistic symptoms in male. Transgenic mice overexpressing the human MECP2 gene exhibit autistic-like behaviors. Neural circuits underlying social defects in MECP2 transgenic(MECP2-TG) mice remain unknown. To observe neural activity of MECP2-TG mice in vivo, we performed calcium imaging by implantation of microendoscope in the hippocampal CA1 regions of MECP2-TG and wild type(WT) mice. We identified neurons whose activities were tightly associated with social interaction, which activity patterns were compromised in MECP2-TG mice. Strikingly, we rescued the social-related neural activity in CA1 and social defects in MECP2-TG mice by deleting the human MECP2 transgene using the CRISPR/Cas9 method during adulthood.Our data points to the neural circuitry responsible for social interactions and provides potential therapeutic targets for autism in adulthood.展开更多
Loop heat pipes （LHPs） were designed for the alpha magnetic spectrometer （AMS-02） to dissipate heat from the cryocoolers. A bypass valve is applied to the LHP to keep the cryocooler temperature above its limit （...Loop heat pipes （LHPs） were designed for the alpha magnetic spectrometer （AMS-02） to dissipate heat from the cryocoolers. A bypass valve is applied to the LHP to keep the cryocooler temperature above its limit （-20℃） in cold environment. Extensive experiments were performed on operation characteristics of LHPs with the bypass valve for AMS-02 during thermal vacuum and thermal balance （TVTB） test. We found that the bypass valve can start up successfully in cold environment. With the bypass valve, the evaporator temperature is stable and can meet the requirement of the cryocooler. We analyzed three operating modes of the bypass valve. Set point temperature and regulation temperature shifts were observed and their relations with the bypass valve temperature were given.展开更多
Brain-specific loss of a microtubule-binding protein collapsin response mediator protein-2(CRMP2)in the mouse recapitulates many schizophrenia-like behaviors of human patients,possibly resulting from associated develo...Brain-specific loss of a microtubule-binding protein collapsin response mediator protein-2(CRMP2)in the mouse recapitulates many schizophrenia-like behaviors of human patients,possibly resulting from associated developmental deficits in neuronal differentiation,path-finding,and synapse formation.However,it is still unclear how the Crmp2 loss affects neuronal circuit function and plasticity.By conducting in vivo and ex vivo electrophysiological recording in the mouse primary visual cortex(V1),we reveal that CRMP2 exerts a key regulation on the timing of postnatal critical period(CP)for experience-dependent circuit plasticity of sensory cortex.In the developing V1,the Crmp2 deficiency induces not only a delayed maturation of visual tuning functions but also a precocious CP for visual input-induced ocular dominance plasticity and its induction activity–coincident binocular inputs right after eye-opening.Mechanistically,the Crmp2 deficiency accelerates the maturation process of cortical inhibitory transmission and subsequently promotes an early emergence of balanced excitatory-inhibitory cortical circuits during the postnatal development.Moreover,the precocious CP plasticity results in deteriorated binocular depth perception in adulthood.Thus,these findings suggest that the Crmp2 deficiency dysregulates the timing of CP for experience-dependent refinement of circuit connections and further leads to impaired sensory perception in later life.展开更多
：Objective：To study the epidemiologic characteristics of traffic injuries among people over 60 years old in the Nan＇ an district（urban）and Jiangjin district（rural）of Chongqing,and to discuss the corresponding s...：Objective：To study the epidemiologic characteristics of traffic injuries among people over 60 years old in the Nan＇ an district（urban）and Jiangjin district（rural）of Chongqing,and to discuss the corresponding strategies for its prevention and cure.Methods：Records of traffic injuries in people over 60 years old registered by the traffic police between 2000 and 2006 in Nan＇an district and Jiangjin district were collected in the Database of Road Traffic Accidents and Traffic Injuries.Epidemiologic characteristics of traffic injuries among the aged people were analyzed and compared.Results：Between the year 2000 and 2006,the average annual incidence of traffic injuries and mortality rate in the aged people in Nan＇ an district were 124.62/100 000 and 13.85/100 000 respectively,higher than that in Jiangjin district（27.49/100 000,7.13/100 000,P〈0.01）.However,the mortality rate for the aged people who were involved in traffic injuries in Jiangjin district was 20.60%,higher than that in Nan＇an district（10.00%,P〈0.01）.Head injury was the primary cause of death.Totally 76.58% of casualties were pedestrians.Over 90% of the traffic accidents occurred in the areas with no traffic signal or traffic control system.Conclusions：The traffic environment is unfavorable to the aged people.It is important to enhance traffic safety consciousness of drivers and the elderly and to strengthen traffic safety system and traffic law,so as to provide a safe road traffic environment for the aged people.展开更多
基金This work was supported by the National Basic Research Program of China(2017YFA0103303)Strategic Priority Research Program of the Chinese Academy of Sciences(XDB32010100,XDB02050400,XDB02050005,XDA16020601)+2 种基金National Basic Research Program of China(2017YFA0102601,2019YFA0110100)National Natural Science Foundation of China(NSFC)(91732301,31671072,31771140,81891001,91432111,81527901,31400977,31625013)Grants of Beijing Brain Initiative of Beijing Municipal Science&Technology Commission(Z181100001518004).
文摘Duplications of MECP2-containing genomic segments led to severe autistic symptoms in male. Transgenic mice overexpressing the human MECP2 gene exhibit autistic-like behaviors. Neural circuits underlying social defects in MECP2 transgenic(MECP2-TG) mice remain unknown. To observe neural activity of MECP2-TG mice in vivo, we performed calcium imaging by implantation of microendoscope in the hippocampal CA1 regions of MECP2-TG and wild type(WT) mice. We identified neurons whose activities were tightly associated with social interaction, which activity patterns were compromised in MECP2-TG mice. Strikingly, we rescued the social-related neural activity in CA1 and social defects in MECP2-TG mice by deleting the human MECP2 transgene using the CRISPR/Cas9 method during adulthood.Our data points to the neural circuitry responsible for social interactions and provides potential therapeutic targets for autism in adulthood.
基金supported by the Major Project of Technology Transfer of Shandong Province (Grant No. 2009ZHZX1A1105)
文摘Loop heat pipes （LHPs） were designed for the alpha magnetic spectrometer （AMS-02） to dissipate heat from the cryocoolers. A bypass valve is applied to the LHP to keep the cryocooler temperature above its limit （-20℃） in cold environment. Extensive experiments were performed on operation characteristics of LHPs with the bypass valve for AMS-02 during thermal vacuum and thermal balance （TVTB） test. We found that the bypass valve can start up successfully in cold environment. With the bypass valve, the evaporator temperature is stable and can meet the requirement of the cryocooler. We analyzed three operating modes of the bypass valve. Set point temperature and regulation temperature shifts were observed and their relations with the bypass valve temperature were given.
基金the National Natural Science Foundation of China(32071025,31921002,and 31730108)the Beijing Municipal Science&Technology Commission(Z181100001518001)+1 种基金the Interdisciplinary Research Fund of Beijing Normal Universitythe Strategic Priority Research Program and Innovation Program of the Chinese Academy of Sciences(XDB32020100)。
文摘Brain-specific loss of a microtubule-binding protein collapsin response mediator protein-2(CRMP2)in the mouse recapitulates many schizophrenia-like behaviors of human patients,possibly resulting from associated developmental deficits in neuronal differentiation,path-finding,and synapse formation.However,it is still unclear how the Crmp2 loss affects neuronal circuit function and plasticity.By conducting in vivo and ex vivo electrophysiological recording in the mouse primary visual cortex(V1),we reveal that CRMP2 exerts a key regulation on the timing of postnatal critical period(CP)for experience-dependent circuit plasticity of sensory cortex.In the developing V1,the Crmp2 deficiency induces not only a delayed maturation of visual tuning functions but also a precocious CP for visual input-induced ocular dominance plasticity and its induction activity–coincident binocular inputs right after eye-opening.Mechanistically,the Crmp2 deficiency accelerates the maturation process of cortical inhibitory transmission and subsequently promotes an early emergence of balanced excitatory-inhibitory cortical circuits during the postnatal development.Moreover,the precocious CP plasticity results in deteriorated binocular depth perception in adulthood.Thus,these findings suggest that the Crmp2 deficiency dysregulates the timing of CP for experience-dependent refinement of circuit connections and further leads to impaired sensory perception in later life.
文摘：Objective：To study the epidemiologic characteristics of traffic injuries among people over 60 years old in the Nan＇ an district（urban）and Jiangjin district（rural）of Chongqing,and to discuss the corresponding strategies for its prevention and cure.Methods：Records of traffic injuries in people over 60 years old registered by the traffic police between 2000 and 2006 in Nan＇an district and Jiangjin district were collected in the Database of Road Traffic Accidents and Traffic Injuries.Epidemiologic characteristics of traffic injuries among the aged people were analyzed and compared.Results：Between the year 2000 and 2006,the average annual incidence of traffic injuries and mortality rate in the aged people in Nan＇ an district were 124.62/100 000 and 13.85/100 000 respectively,higher than that in Jiangjin district（27.49/100 000,7.13/100 000,P〈0.01）.However,the mortality rate for the aged people who were involved in traffic injuries in Jiangjin district was 20.60%,higher than that in Nan＇an district（10.00%,P〈0.01）.Head injury was the primary cause of death.Totally 76.58% of casualties were pedestrians.Over 90% of the traffic accidents occurred in the areas with no traffic signal or traffic control system.Conclusions：The traffic environment is unfavorable to the aged people.It is important to enhance traffic safety consciousness of drivers and the elderly and to strengthen traffic safety system and traffic law,so as to provide a safe road traffic environment for the aged people.
