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基于RPA-CRISPR-Cas12a和聚噻吩显色技术快速检测转基因植物外源基因CP4-EPSPS 被引量:5
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作者 刘华 曾海娟 +3 位作者 唐雪明 徐丹红 雒嘉伟 王金斌 《食品安全质量检测学报》 CAS 北大核心 2022年第23期7758-7764,共7页
目的开发基于重组酶恒温扩增(recombinase polymerase amplification,RPA)结合簇状规则间隔短链重复序列及相关蛋白(clustered regularly interspaced short palindromic repeats-associated 12a protein,CRISPR-Cas12a)和聚噻吩显色技... 目的开发基于重组酶恒温扩增(recombinase polymerase amplification,RPA)结合簇状规则间隔短链重复序列及相关蛋白(clustered regularly interspaced short palindromic repeats-associated 12a protein,CRISPR-Cas12a)和聚噻吩显色技术快速检测转基因植物外源基因CP4-EPSPS的方法。方法利用Cas12a附属切割机制进行精准识别RPA产物中的靶标,结合阳离子水溶性共轭聚噻吩(cationic water-soluble conjugated polythiophene,PMNT)与体系中单链DNA(single stranded DNA,ssDNA)的颜色变化检测转基因产品中耐除草剂草甘膦CP4-EPSPS抗性基因。结果167 bp的RPA引物进行扩增时,扩增体系为20μL时条带最清晰;ssDNA的碱基数为15,Cas12a酶的浓度为0.28 U为最优组合。进行RPA-CRISPR-Cas12a反应后,加入PMNT溶液,裸眼观察是溶液从红色变为黄色,紫外下照射下则颜色为橘红色变为橙黄色,检出限为45拷贝。结论构建了一种基于PMNT颜色变化进行转基因产品的CRISPR-Cas12a的检测方法,30 min内完成整个检测过程,仅需要恒温加热可实现快速灵敏、可视化的检测。 展开更多
关键词 重组酶恒温扩增 簇状规则间隔短链重复序列 阳离子水溶性共轭聚噻吩 现场速测 转基因产品
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过磷酸钙中有效磷含量快速测定方法研究 被引量:2
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作者 卢扬 唐绪全 +6 位作者 杨静 周光全 黄师卫 张正华 朱昌峰 胡艳燕 杨剑虹 《西南农业大学学报(自然科学版)》 CSCD 北大核心 2006年第2期223-226,247,共5页
用pH=11的0.1moL/L EDTA溶液在常温条件下1次提取普通过磷酸钙中有效磷,用钼黄比色法测定提取液中的磷。测定结果经校正后得到现场速测值。与标准值对照,回收率90%~110%,标准差0.192-0.780。方法操作简单,耗时不超过35min,... 用pH=11的0.1moL/L EDTA溶液在常温条件下1次提取普通过磷酸钙中有效磷,用钼黄比色法测定提取液中的磷。测定结果经校正后得到现场速测值。与标准值对照,回收率90%~110%,标准差0.192-0.780。方法操作简单,耗时不超过35min,适用于现场样品快速检测。 展开更多
关键词 过磷酸钙 有效磷 现场速测
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Ground-borne vibration generated by high-speed train viaduct systems in soft-upper/hard-lower rock strata 被引量:4
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作者 XING Meng-ting WANG Ping +2 位作者 ZHAO Cai-you WU Xue KANG Xiu-shan 《Journal of Central South University》 SCIE EI CAS CSCD 2021年第7期2140-2157,共18页
An experimental study and theoretical analysis were carried out to explore the ground-borne vibration generated by elevated high-speed railway in rock strata.Taking a typical rail line constructed on rock area in Chin... An experimental study and theoretical analysis were carried out to explore the ground-borne vibration generated by elevated high-speed railway in rock strata.Taking a typical rail line constructed on rock area in China as the research object,a set of field tests was performed on Rizhao-Lankao High-Speed Railway,the bridge and ground vibrations were measured as trains passed at 330−340 km/h,then the transferring law and spatial distribution under individual frequencies were investigated.The experiment results indicate that the bridge frequency spectrum exhibited relatively high-frequency vibration peaks caused by short-wavelength irregularity;ground vibration farther than 30 m away can be amplified with a higher frequency and numerous components.Furthermore,the wave propagation equation of a stratified rock strata was established based on direct-stiffness method to explore the vibration attenuation rules via frequency-domain analysis.It is found that the rock area has a weaker correlation between vibration transmissibility and frequency,thicker and harder rock strata loss their vibration attenuation capacity.It can be concluded that the high-speed railways induced vibration on rock strata shows a wide frequency band and large amplitude,the design of reducing vibration aimed at specific frequency is important according to next more detailed numerical study. 展开更多
关键词 rock stratum vibration and wave elevated high-speed railways in situ test transferring law
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Performance and Adaptation of the Vallerani Mechanized Water Harvesting System in Degraded Badia Rangelands
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作者 I.A. Gammoh T.Y. Oweis 《Journal of Environmental Science and Engineering》 2011年第10期1370-1380,共11页
Rainwater harvesting in micro-catchments such as contour ridges and semicircular bunds is an option for utilizing the limited rainfall, improving productivity and combating land degradation in dry rangeland areas (Ba... Rainwater harvesting in micro-catchments such as contour ridges and semicircular bunds is an option for utilizing the limited rainfall, improving productivity and combating land degradation in dry rangeland areas (Badia). However, implementation of this practice using manual labor or traditional machinery is slow, tedious and costly, and often impractical on a large scale. These limitations can be overcome using the "Vallerani" plow for quickly constructing continuous and intermittent ridges. The plow (model Delfino (50 MI/CM), manufactured by Nardi, Italy) was tested and adapted to dry steppe (Badia) conditions in Jordan. The performance of the machine, its weaknesses and potential improvements were assessed in the 2006/07 season at three sites on 165 hectares of various terrain, slope and soil conditions. The performance parameters included effective field capacity (EFC), machine efficiency (ME) and fuel consumption (FC). Field tests were carried out at different tractor (134 HP) traveling speeds, pit sizes and contour spacings. Overall mean performance indicators gave an EFC of 1.2 ha/h, 51% ME and an average FC of 5.15 liter/ha. Increasing ridge spacing had a small effect on ME where, increasing traveling speed had a greater effect. A guide table was developed, relating performance parameters with ridge spacing, speed, and bund size setting. This could be a useful reference for the implementation and management of mechanized micro-catchment construction in the Badia. The system performed well in the construction of continuous ridges. However, it was unable to construct intermittent ridges at speeds over 4km/h; problems were encountered in properly staggering the bunds at successive contours. 展开更多
关键词 Land degradation contour micro-catchments Vallerani system machine capacity machine efficiency Badia.
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Research on Influence of Electromagnetic Interference on X-Ray Digital Detection
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作者 Guo Taotao Wang Dada +1 位作者 Guo Tieqiao Yu Hong 《Electricity》 2012年第5期38-43,共6页
X-ray digital imaging technology has found wide application owing to its advantages of real-time, visualization and rapid imaging. In substations the substantial electromagnetic interference has some influence on the ... X-ray digital imaging technology has found wide application owing to its advantages of real-time, visualization and rapid imaging. In substations the substantial electromagnetic interference has some influence on the live detection by the X-ray digital imaging technology, hindering the promotion of the technology in the detection of electric equipment. Based on a large number of field tests, the author carded out a series of researches on electromagnetic interference protection measures, image de-noising, and image enhancement algorithms. 展开更多
关键词 SUBSTATION live detection X-RAY digital imaging electromagnetic interference image de-noising image enhancement
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The nonresonant perturbation theory based field measurement and tuning of a linac accelerating structure 被引量:3
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作者 FANG WenCheng TONG DeChun +4 位作者 GU Qiang ZHAO ZhenTang SHENG Xing CHEN LiFang WANG Lin 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS 2013年第11期2104-2109,共6页
Assisted by the bead pull technique,nonresonant perturbation theory is applied for measuring and tuning the field of the linac accelerating structure.The method is capable of making non-touching amplitude and phase me... Assisted by the bead pull technique,nonresonant perturbation theory is applied for measuring and tuning the field of the linac accelerating structure.The method is capable of making non-touching amplitude and phase measurements,real time mismatch feedback and field tuning.Some key considerations of the measurement system and of a C-band traveling-wave structure are discussed,and the bead pull measurement and the tuning of the C-band traveling-wave linac accelerating structure are presented at last. 展开更多
关键词 nonresonant perturbation accelerating structure RF test tuning
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