Pollen analysis of 30 modem water samples from the Shivang River, an internal river system located between the Tengger and Badain Jaran deserts, Northwest China was carried out to examine the river's capacity to c...Pollen analysis of 30 modem water samples from the Shivang River, an internal river system located between the Tengger and Badain Jaran deserts, Northwest China was carried out to examine the river's capacity to carry pollen and spores, and to assess the contribution of the water-borne pollen to pollen assemblages in lake sediments at the end of the river system. Results indicate the pollen assemblages in water samples consist of both local and upland pollen. Percentages of upland pollen reach 30% - 60%, and pollen assemblages in water samples do not indicate the nature of local vegetation at the sampling sites. Fluvial currents have the capacity to transport large quantities of pollen long distances, and the contribution of this fluvial transported pollen is relatively high, For example, percentages of Picea Dietr. pollen in water samples at sampling sites 130 km and 145 km away from Picea forests reach 16.5% and 7.7%, respectively. Fluvial pollen transport occurs primarily during flood periods, and pollen concentrations from the flood samples are 17.1 - 12.5 times those from normal fluvial flow. Reservoirs affect pollen transportation since pollen is deposited at reservoir inlets and pollen concentrations are much reduced at reservoir outlets. Human activity can thus change natural features of pollen transportation and deposition. The main factors influencing pollen concentrations and assemblages are sampling time, sampling location, and rainfall intensity.展开更多
Objective: To elucidate the relation between human tissue factor pathwayinhibitor-2 (TFPI-2) expression and ovarian tumor migration and invasion. Methods: Human TFPI-2expression vector pBos-Cite-neo/TFPI-2 was transfe...Objective: To elucidate the relation between human tissue factor pathwayinhibitor-2 (TFPI-2) expression and ovarian tumor migration and invasion. Methods: Human TFPI-2expression vector pBos-Cite-neo/TFPI-2 was transfected into ovarian tumor cells line A2780- Afterthe transfected cells were selected by G418, transfected and nontransfected cells were screened forTFPI-2 mRNA and protein by reverse transcription-polymerase chain reaction and Western blotanalysis, respectively. The number of transfected or nontransfected cells passing through membraneof Boyden chamber was counted as the basis assessing tumor cells migratory and invasive behaviors.Results: Expression of mRNA and protein of TFPI-2 was detectable in transfected cells. In invasionassay, the number of TFPI-2-expressing cells to traverse a Matrigel-coated membrane was obviouslydecreased compared with that of nonexpressing cells (59.3±6.5 vs 109.7±5.5, P 【 0.01); While inmigration assay, no significant difference through a noncoated membrane was observed amongtransfected and nontransfected cells (114.7±8.6 vs 127.3±7.1, P 】 0.05). Conclusion: Expression ofTFPI-2 may strongly inhibit the invasive ability of ovarian tumor cells in vitro, but has no effecton the migratory ability which provides an experimental basis for genotherapy of human ovariantumor.展开更多
文摘Pollen analysis of 30 modem water samples from the Shivang River, an internal river system located between the Tengger and Badain Jaran deserts, Northwest China was carried out to examine the river's capacity to carry pollen and spores, and to assess the contribution of the water-borne pollen to pollen assemblages in lake sediments at the end of the river system. Results indicate the pollen assemblages in water samples consist of both local and upland pollen. Percentages of upland pollen reach 30% - 60%, and pollen assemblages in water samples do not indicate the nature of local vegetation at the sampling sites. Fluvial currents have the capacity to transport large quantities of pollen long distances, and the contribution of this fluvial transported pollen is relatively high, For example, percentages of Picea Dietr. pollen in water samples at sampling sites 130 km and 145 km away from Picea forests reach 16.5% and 7.7%, respectively. Fluvial pollen transport occurs primarily during flood periods, and pollen concentrations from the flood samples are 17.1 - 12.5 times those from normal fluvial flow. Reservoirs affect pollen transportation since pollen is deposited at reservoir inlets and pollen concentrations are much reduced at reservoir outlets. Human activity can thus change natural features of pollen transportation and deposition. The main factors influencing pollen concentrations and assemblages are sampling time, sampling location, and rainfall intensity.
文摘Objective: To elucidate the relation between human tissue factor pathwayinhibitor-2 (TFPI-2) expression and ovarian tumor migration and invasion. Methods: Human TFPI-2expression vector pBos-Cite-neo/TFPI-2 was transfected into ovarian tumor cells line A2780- Afterthe transfected cells were selected by G418, transfected and nontransfected cells were screened forTFPI-2 mRNA and protein by reverse transcription-polymerase chain reaction and Western blotanalysis, respectively. The number of transfected or nontransfected cells passing through membraneof Boyden chamber was counted as the basis assessing tumor cells migratory and invasive behaviors.Results: Expression of mRNA and protein of TFPI-2 was detectable in transfected cells. In invasionassay, the number of TFPI-2-expressing cells to traverse a Matrigel-coated membrane was obviouslydecreased compared with that of nonexpressing cells (59.3±6.5 vs 109.7±5.5, P 【 0.01); While inmigration assay, no significant difference through a noncoated membrane was observed amongtransfected and nontransfected cells (114.7±8.6 vs 127.3±7.1, P 】 0.05). Conclusion: Expression ofTFPI-2 may strongly inhibit the invasive ability of ovarian tumor cells in vitro, but has no effecton the migratory ability which provides an experimental basis for genotherapy of human ovariantumor.
