Linkers were assembled on a glass surface based on the hydrolysis and condensation of 3-glycidoxy￣propyltrimethoxysilane (GPS). After the assembly of GPS, four approaches were tried to open the ending epoxide group o...Linkers were assembled on a glass surface based on the hydrolysis and condensation of 3-glycidoxy￣propyltrimethoxysilane (GPS). After the assembly of GPS, four approaches were tried to open the ending epoxide group of GPS or to further elongate the linkers. The effect of these approaches on DNA in situ synthesis and hybridization was investigated. For the spacing of the synthesis initiation sites, the wettability of the support and the length of the linking group that attaches the initiation site to the surface have direct influences on the yield of coupling reactions and the subsequent hybridization events. X-ray photoelectron spectroscopy (XPS) and mean contact angles of deionized water of the above slides were measured to assess the linker's characteristics in each procedure. It was proved that the glass slides were successfully modified and became excellent supports for the oligonucleotides synthesis. In addition, it proved best for the in situ oligonucleotides synthesis that a glass slide was in turn treated with ethylenediamine, glutaradehyde, ethanolamine and sodium borohydride solution at ambient temperature after silanized with GPS.展开更多
Effects of four kinds of triazine herbicides on the photodegradation of acetochlor were studied on glass surface and in aqueous solution under three kinds of light sources, i.e., xenon lamp, high pressure mercury lamp...Effects of four kinds of triazine herbicides on the photodegradation of acetochlor were studied on glass surface and in aqueous solution under three kinds of light sources, i.e., xenon lamp, high pressure mercury lamp and natural sunlight. The results indicated that atrazine, simetryne, prometryne and ametryne exhibited significant effects on the photoquenching degradation of acetochlor, and there was an obvious positive correlation between their effects and the dosage of four kinds of triazine herbicides.展开更多
文摘Linkers were assembled on a glass surface based on the hydrolysis and condensation of 3-glycidoxy￣propyltrimethoxysilane (GPS). After the assembly of GPS, four approaches were tried to open the ending epoxide group of GPS or to further elongate the linkers. The effect of these approaches on DNA in situ synthesis and hybridization was investigated. For the spacing of the synthesis initiation sites, the wettability of the support and the length of the linking group that attaches the initiation site to the surface have direct influences on the yield of coupling reactions and the subsequent hybridization events. X-ray photoelectron spectroscopy (XPS) and mean contact angles of deionized water of the above slides were measured to assess the linker's characteristics in each procedure. It was proved that the glass slides were successfully modified and became excellent supports for the oligonucleotides synthesis. In addition, it proved best for the in situ oligonucleotides synthesis that a glass slide was in turn treated with ethylenediamine, glutaradehyde, ethanolamine and sodium borohydride solution at ambient temperature after silanized with GPS.
文摘Effects of four kinds of triazine herbicides on the photodegradation of acetochlor were studied on glass surface and in aqueous solution under three kinds of light sources, i.e., xenon lamp, high pressure mercury lamp and natural sunlight. The results indicated that atrazine, simetryne, prometryne and ametryne exhibited significant effects on the photoquenching degradation of acetochlor, and there was an obvious positive correlation between their effects and the dosage of four kinds of triazine herbicides.
